Biochem Biophys Rep. 2026 Mar;45
102493
An in vitro model of classical conditioning could improve our understanding of underlying mechanisms thanks to controlled pharmacological manipulation and negligeable perturbations from external networks. Here, using mice cerebellar slices and patch clamp recordings, we present a protocol for classical conditioning of Purkinje cells in vitro at room temperature. Repeated pairing of a parallel fiber conditional stimulus and a climbing fiber unconditional stimulus with parameters compatible with in vivo learning gives rise to a Purkinje cell simple spike suppression during the conditional stimulus that shares similarities with conditioned responses observed in live animals.