bims-enlima Biomed News
on Engineered living materials
Issue of 2026–02–08
fifty-four papers selected by
Rahul Kumar, Tallinna Tehnikaülikool



  1. Adv Sci (Weinh). 2026 Jan 30. e20526
      Life-like materials that can dynamically morph their shape/texture, inspired by living organisms such as cephalopods are sought after for soft robotics and camouflage applications. Achieving such functions demands multimaterials with spatially programmed responses, yet their creation remains challenging. The existing approaches have been limited by the specific in situ polymerization conditions, fluidity of the synthetic components, and the complex, multi-step processing requirements. Here, we propose a universal strategy that enables programming of material response within pre-synthesized, ready-to-use, clickable microgels with different response behaviors. These microgels can be deposited within desired regions of the material structure via direct ink writing to enable pre-programmed, localized responses to external stimuli. Spontaneous interparticle stabilization of the deposited microgels via a click reaction (Diels-Alder bonding) yields shape-stable, free-form granular hydrogel multimaterials with reversible, repeatable, and spatially selective responses to stimuli (e.g., pH and temperature). The strategy establishes a 4D-printing-compatible, scalable modular platform for facile fabrication of soft materials with programmable shape adaptivity.
    Keywords:  4D printing; Dynamic covalent bonds; Microgel assembly; Multi‐responsive hydrogels; Programmable materials; Spatioselective response
    DOI:  https://doi.org/10.1002/advs.202520526
  2. Nat Commun. 2026 Feb 02.
      Cells contain membrane-bound and membraneless organelles that operate as spatially distinct biochemical niches. However, these reaction centers lose fidelity due to aging or diseases. A grand challenge for biomedicine is restoring or augmenting cellular functionalities. An excited strategy is the delivery of protein-based materials that can directly interact with cellular biological networks. In this study, we sought to develop long-lasting materials capable of cellular uptake, akin to intracellular interaction hubs. We develop a delivery method to efficiently transplant stable micron-size peptide-based compartments into living cells. By loading coacervates with nanobodies and bioPROTACs, we demonstrate successful target sequestration of natively expressed GFP to our synthetic hubs, and function as bioreactors to selectively degrade GFP inside human cells. These results represent an important step toward the development of synthetic organelles that can be fabricated in vitro and taken up by cells for applications in cell engineering and regenerative medicine.
    DOI:  https://doi.org/10.1038/s41467-026-68793-7
  3. Nat Commun. 2026 Feb 02.
      Plasmids remain by far the most common medium for delivering engineered DNA to microorganisms. However, the reliance on natural plasmid replication mechanisms limits their tunability, compatibility, and modularity. Here we refactored the natural pMB1 origin and created plasmids with customizable copy numbers by tuning refactored components. We then created compatible origins that use synthetic RNA regulators to implement independent copy control. We further demonstrated that the synthetic origin of replication (SynORI) can be engineered modularly to respond to various signals, allowing for multiplexed copy-based reporting of environmental signals. Lastly, a library of 6 compatible SynORI plasmids was created and co-maintained in E. coli for a week. This work establishes the feasibility of creating plasmids with SynORI that can serve as a biotechnology for synthetic biology.
    DOI:  https://doi.org/10.1038/s41467-026-68907-1
  4. Adv Mater. 2026 Feb 06. e19692
      Plant-based, iridescent, and dynamically tunable structural colored materials are highly attractive for sustainable photonic devices. However, fabricating complex architectures at the decimeter-scale with optical fidelity using plant-derived materials remains challenging, limiting their use in photonic devices and adaptive actuation. Here, we introduce an aqueous two-phase freeform fabrication strategy for vibrantly colored hydroxypropyl cellulose (HPC), where a robust immiscible aqueous environment is developed to preserve HPC cholesteric structures with < 3% shift in peak reflection wavelength over three days, enabling stable processing of large-scale structural colored materials. Our technique involves a food-grade support medium with low interfacial tension, allowing for embedded 3D printing of photonic structures and post-extrusion recovery of the HPC cholesteric domains. Intricate constructs, including interlocking chainmail, with feature sizes down to ∼50 µm and color consistency over lengths exceeding ten centimeters, can be achieved. Additionally, this approach can be utilized to create non-planar, mechanochromic hydrogel actuators with programmable multicolor designs, as demonstrated in an octopus-inspired hydrogel actuator and a color-shifting display for information encryption, camouflage, and human-machine interaction. Our green, freeform manufacturing approach provides new design possibilities for sustainable photonic devices and can be applied to industrially relevant applications.
    Keywords:  aqueous two‐phase systems; embedded 3D printing; freeform structures; green manufacturing; hydroxypropyl cellulose; structural color
    DOI:  https://doi.org/10.1002/adma.202519692
  5. Adv Mater. 2026 Feb 03. e18567
      How can synthetic polymers be endowed with the continuous, life-like ability to grow, degrow, heal, and alter their chemical and physical properties after fabrication? This study addresses this question by coupling theory and experiment to create an open-system "living" polymer platform that integrates mass transport, reversible polymerization, chain exchange, and evolving elasticity into a fully chemomechanically coupled network. Controlled transport, reaction, and stresses enable continuous growth and degrowth with microscale control enabled by light-activated catalysts. Their chemical composition can be reprogrammed on demand, tuning modulus by up to two orders of magnitude to either stiffen or soften the material. These capabilities enable self-growable electronics, transformative soft robots, and on-site damage-regenerating devices, establishing a foundation for sustainable, endlessly reprogrammable polymers.
    Keywords:  chemomechanics; dynamic polymers; growable soft electronics; transformable soft robots
    DOI:  https://doi.org/10.1002/adma.202518567
  6. bioRxiv. 2026 Jan 12. pii: 2026.01.10.698826. [Epub ahead of print]
      Hydrogels serve as powerful models for investigating cell-extracellular matrix (ECM) interactions. While chemical modifications are routinely used to tune hydrogel properties, it remains unclear whether these modifications mediate cell fate. Previous work has shown that cells deposit newly synthesized (nascent) ECM at the cell-hydrogel interface. Here, we demonstrate that this nascent ECM interface regulates how cells interpret chemical modifications. Using hydrogels with varied chemical modifications, we isolated the effects of chemical modification on nascent ECM and cell fate. Nascent ECM deposition increased as a function of hydrogel modification and with distinct matrisome compositions. While low modification hydrogels promoted cell differentiation, high modifications increased cell proliferation. Perturbing cell-nascent ECM interactions reversed this cell fate. Our findings reveal that nascent ECM regulates cell fate by converting hydrogel cues into signals that control cell fate. This tri-directional interplay among hydrogel chemical modifications, nascent ECM, and cell fate reframes how we interpret cell-hydrogel interactions.
    DOI:  https://doi.org/10.64898/2026.01.10.698826
  7. Small. 2026 Feb 06. e09658
      Post-translational modifications (PTMs) of proteins are used by natural systems to expand beyond the 20 canonical amino acids. The variation introduced at the sequence level by PTMs after expression leads to changes in both the structure and function of proteins. PTMs expand the chemical repertoire from which new biomaterials can be constructed. Inspired by the post-translational conjugation of cholesterol to proteins, we have synthesized five new hybrid lipid-protein biomaterials called Sterol-modified polypeptides (STaMPs). These STaMPs consist of an elastin-like polypeptide (ELP) conjugated to a sterol, namely coprostanol, epicoprostanol, androstanol, galeterone, or dehydroepiandrosterone. We show that STaMPs exhibit sterol-dependent self-assembly behavior, ranging from predominantly monomeric random coils for the most hydrophilic sterols to spherical micelles for the most hydrophobic sterols. Furthermore, the sterols modify the typical LCST behavior of ELPs in a predictable fashion depending on the hydrophobicity of the sterol appended.
    Keywords:  elastin‐like polypeptides; lipidation; post‐translational modifications; protein engineering; self‐assembly
    DOI:  https://doi.org/10.1002/smll.202509658
  8. Angew Chem Int Ed Engl. 2026 Feb 05. e26058
      Dynamic polymer networks (DPN) leverage transient cross-linking to yield macroscopic materials that exhibit self-healing and environmentally responsive behaviors. Despite the broad scope of chemical interactions available for cross-linking, imbuing those transient interactions into biologically compatible materials is difficult because many chemistries are incompatible with aqueous conditions, and it is difficult to tune preexisting biological interactions that have evolved over millions of years for specificity. To enable the assembly of chemically tunable and biologically compatible DPNs, we have developed a set of bifunctional, heteroaffinity cross-linkers (HAX) where the reactive moieties have different binding affinities to the same binding sites of an oligomeric protein. The use of cross-linking moieties with vastly different dissociation rates enables purification of protein modules with monodisperse HAX valencies. Assembly of DPNs from stoichiometrically identical pairs of protein modules then yields unique, metastable, nonequilibrium network topologies. Here, we demonstrate these concepts using the well-studied avidin-biotin interaction chemistry. We also develop a pH-sensitive HAX that yields DPNs with robust pH-responsive assembly dynamics, and demonstrate how this DPN can be made into a magnetically responsive, molecular delivery system to low-pH regions, such as tumor microenvironments.
    Keywords:  NMR spectroscopy; biophysics; nonequilibrium processes; polymers; self‐assembly
    DOI:  https://doi.org/10.1002/anie.202526058
  9. Nature. 2026 Feb;650(8100): 10
      
    Keywords:  Biotechnology; Catalysis
    DOI:  https://doi.org/10.1038/d41586-026-00275-8
  10. Sci Adv. 2026 Feb 06. 12(6): eadz7415
      Three-dimensional (3D) printing can create freeform architectures and electronics with unprecedented versatility. However, the full potential of electronic 3D printing has so far been limited by the inability to selectively anneal the printed materials, especially on temperature-sensitive substrates. Here, we achieve highly selective and rapid volumetric heating of 3D-printed nanomaterials and polymers in situ by focusing microwaves using a metamaterial-inspired near-field electromagnetic structure (Meta-NFS). In contrast to previous work, the Meta-NFS achieves the spatial resolution and power density needed to 3D print freeform microstructures where the electronic and mechanical properties can be locally programmed even within optically opaque materials. By broadening the material palettes compatible with 3D printing, near-field microwave 3D printing with Meta-NFS enables classes of electronics that are otherwise challenging to create.
    DOI:  https://doi.org/10.1126/sciadv.adz7415
  11. Macromol Rapid Commun. 2026 Jan 31. e00868
      Modulating biomaterial properties using light holds great promise for biomedical applications, such as drug delivery, as it is non-invasive and offers both spatial and temporal control. Visible light is particularly salient for stimulation of cell-interfacing materials, as it is cyto-compatible; however, this limits the number of photoswitches appropriate for these applications. In this work, we use donor-acceptor Stenhouse adduct (DASA) functionalized polymers comprising poly(ethylene glycol)-b-poly(hexyl methacrylate) to make visible light-responsive polymersomes, and use these to encapsulate a model drug cargo. We demonstrate that release of the model cargo can be triggered using visible light when the polymersomes are loaded into poly(ethylene glycol) hydrogels. Moreover, ON/OFF switchable cargo release was demonstrated by modulating the light stimulation of the hydrogel. We envisage this could be used to dynamically modulate hydrogel properties in clinically relevant applications for controlled delivery of small molecule therapeutic agents, such as advanced in vitro tissue models and implantable drug-eluting scaffolds.
    Keywords:  Donor–acceptor Stenhouse adducts; Drug release; Hydrogels; Photoswitches; Polymersomes
    DOI:  https://doi.org/10.1002/marc.202500868
  12. ACS Appl Bio Mater. 2026 Feb 04.
      The ability of tendons to transmit forces from muscle to bone is fundamentally attributed to the hierarchical anisotropy of the tissue. After injury, disorganized fibrotic scar tissue forms during the natural healing process, resulting in inferior mechanical properties that often lead to reinjury and limited restoration of function. Therefore, intervention is necessary to facilitate regenerative healing of the tendon. Polymeric biomaterials have historically been used to guide cell behavior, showing promise for the use of topological guidance and cell-mediated matrix remodeling as mechanisms for promoting regeneration. Here, we fabricated 3D scaffolds for tenocytes using anisotropic poly(ethylene glycol)-based hydrogels that recapitulate both the biophysical and biochemical properties of the native tendon. These materials were synthesized using a two-stage polymerization strategy that includes an initial cross-linking step facilitated by thiol-Michael addition, an intermediate mechanical stretching step to align the polymer network, and a second-stage crosslinking step facilitated by a thiol-ene reaction. The application of 300% strain during the mechanical alignment of the network resulted in highly oriented materials (S = 0.38). Furthermore, a matrix metalloproteinase (MMP)-degradable peptide was incorporated into the network to facilitate cell-mediated remodeling of the scaffold. After 14 days of exposure to exogenous MMP2, a sufficient number of cross-links were degraded for alignment to be lost (S = 0.03). When tenocytes were encapsulated in the 3D anisotropic hydrogels, they adopted the anisotropic morphology of the polymer network and deposited an extracellular matrix mainly comprised of type I collagen, indicating a pro-regenerative environment. Comparatively, isotropic materials of the same composition induced a random orientation of encapsulated tenocytes, and a matrix primarily comprised of collagen III was deposited, indicating a fibrotic environment. Collectively, these results demonstrate the successful use of a synthetic scaffold with tunable biophysical and biochemical properties for recapitulating the native tendon environment and promoting regenerative cell behavior.
    Keywords:  anisotropic materials; extracellular matrix; hydrogels; polymer synthesis; tendon
    DOI:  https://doi.org/10.1021/acsabm.5c02408
  13. Mater Today Bio. 2026 Apr;37 102818
      Direct injection of hydrogels loaded with therapeutics holds great promise for tissue regeneration; however, injectable hydrogels typically fill defect spaces without spatiotemporal control, which is critical for regenerating certain tissues. Conversely, 3D printing enables the fabrication of patterned hydrogel constructs but often requires invasive surgical implantation. Here, we present a novel strategy for the non-invasive delivery of 3D-printed constructs. Specifically, we developed gallic acid-modified hyaluronic acid (HA) that was crosslinked for the first time using potassium iodide (KI) as a catalyst, without the need for an initiator or light exposure. This also enabled protein conjugation with gelatin and collagen to obtain an extracellular matrix (ECM)-mimetic ink for 3D printing. We determined the distinct pKa values of the phenolic hydroxy groups of gallol-modified HA, which were utilized to achieve 3D printing at acidic pH, followed by efficient solution-free covalent crosslinking using ammonia gas to ensure complete crosslinking. This approach enabled efficient printing through fine nozzles (G32) and produced robust structures. The printed scaffolds were subsequently loaded into a larger needle and injected, demonstrating shape-memory properties by retaining their geometry post-injection. Furthermore, the scaffolds supported stem cell coating, where the stemness and differentiation of stem cells could be modulated by hydrogel composition and culture conditions, including chondrogenic differentiation towards cartilage-like constructs using TGF-β3. This strategy offers a versatile platform for developing HA-based hydrogels capable of protein conjugation, 3D printing, cell or biomolecule coating, and minimally invasive implantation while maintaining structural fidelity.
    Keywords:  3D printing; Gallic acid; Hyaluronic acid; Hydrogel; Shape-memory
    DOI:  https://doi.org/10.1016/j.mtbio.2026.102818
  14. Adv Mater. 2026 Feb 07. e72447
      Biological tissues and engineered composites achieve exceptional mechanical properties through microstructures that create stiffness and toughness in preferred directions. While composites traditionally leverage solid reinforcements to drive this anisotropy, directional mechanics in all-soft matter composites remain a longstanding challenge, despite their importance for soft devices that stretch and adapt under load. Here, we create all-soft matter composites where liquid inclusions direct and enable anisotropic and heterogeneous mechanical properties. By shaping and orienting liquid metal droplets within elastomers, we program directional stiffness, enhance toughness beyond 36,000 J m-2, and guide cracks along non-linear paths with deflections up to 150 ∘$^\circ$ during extreme deformations. This allows liquids, which are up to a million times softer than traditional rigid inclusions, to act as mechanical reinforcements. These liquid inclusions enhance directional stiffness or softness relative to unfilled elastomers and enable programmable crack-path engineering that surpasses simple blunting or trapping, with anisotropy tuned on demand during processing. We leverage this to protect soft circuits even under catastrophic damage, offering new possibilities to direct mechanical forces in compliant materials for resilient soft electronics and robots, wearables, and morphing matter.
    Keywords:  all‐soft composite; crack steering; liquid metal; mechanical anisotropy
    DOI:  https://doi.org/10.1002/adma.72447
  15. bioRxiv. 2026 Jan 17. pii: 2026.01.16.699845. [Epub ahead of print]
      Asparagine-linked protein glycosylation is among the most frequent modifications of proteins trafficking through the secretory pathway. These glycans are manufactured in an assembly line process to a common precursor that is then subject to individual modifications with different levels of complexity. An important biosynthetic modulator is the incorporation of N -acetylglucosamine (GlcNAc) at distinct positions in N-linked glycan biosynthesis, commencing with the activity of the glycosyltransferase MGAT1. While mapping of N-glycans to their corresponding protein attachment sites is generally possible, not much is known about the glycoprotein substrate choice for MGAT1 and related transferases. Analogs of GlcNAc with small bioorthogonal tags can be incorporated into N-glycans. However, due to the promiscuity of some GlcNAc transferases, incorporation is of little specificity towards individual positions. Here, we report an iterative bump-and-hole approach in the design of a bioorthogonal precision tool for the activity of MGAT1 in mammalian cells. Structure-informed protein engineering abrogated the activity of MGAT1 towards the nucleotide-sugar UDP-GlcNAc while retaining activity towards bumped, azide-modified analogs. Kinetic and computational analyses using a neural network approach informed the synthesis of a tailored UDP-GlcNAc analog with preferential acceptance by the engineered enzyme. Following substrate biosynthesis, the strategy allowed selective incorporation of a chemical tag on MGAT1 substrate proteins in living mammalian cells with little background incorporation by other GlcNAc transferases. Our work expands the toolbox for glycan-based reporter compounds.
    DOI:  https://doi.org/10.64898/2026.01.16.699845
  16. Mater Today Bio. 2026 Apr;37 102799
      Hydrogels emerged as versatile biomaterials for tissue engineering due to their extra cellular matrix similarity and mechanical and biochemical properties. Still, hydrogels expose limited stiffness, anisotropy and nutrient diffusion. By reinforcing hydrogels with synthetic and natural fibers, these drawbacks can be effectively addressed, thereby enabling the modeling of advanced biomimetic tissue. This review discusses recent progress in the fabrication of fiber-integrated hydrogels and brings together developments from biomaterials, biofabrication, mechanobiology, and organ-model engineering. Fiber-addition impact on viscoelastic, time-dependent und nonlinear material properties, on multiscale and hierarchical constructs and on mechanical and biological readouts are analyzed. Specifically, the integration of both synthetic and natural fibers into hydrogel matrices is highlighted which significantly broaden their structural and biochemical versatility. These fiber-added hydrogels display improved properties including enhanced stiffness (up to 10-fold increase), anisotropy (>80 % alignment) and nutrient diffusion (4-fold increase). Moreover, the incorporation of fibers directly impacts cellular behavior by promoting adhesion, migration, proliferation and differentiation. Finally, bone, muscle and nerve tissue are exemplary presented in more detail to highlight the broad potential of these composite materials. In conclusion, fiber-embedded hydrogels represent a decisive step toward enhanced 4D-metamaterials.
    Keywords:  4D-metamaterials; Bone; Fibers; Hydrogels; Muscle; Nerve; Tissue engineering
    DOI:  https://doi.org/10.1016/j.mtbio.2026.102799
  17. Nat Commun. 2026 Feb 03.
      Bacterial cellulose possesses excellent biocompatibility and mechanical strength but lacks the bioactivity needed for many biomedical and healthcare applications. To address this limitation, we develop a metabolic glycoengineering-click chemistry strategy that enables in situ incorporation of azide groups into bacterial cellulose, followed by mild and selective conjugation of alkyne-bearing functional molecules. This approach avoids harsh chemical treatments, preserves the native properties of bacterial cellulose, and supports stable attachment of diverse bioactive agents, including antibacterial porphyrins, arginine-glycine-aspartic acid peptides, and recombinant proteins with fluorescent or enzymatic functions. As a proof-of-concept, a cascade catalytic system comprising glucose oxidase and superoxide dismutase is immobilized onto azide-modified bacterial cellulose, yielding a multifunctional wound dressing designed to address hyperglycemia and oxidative stress-key barriers to chronic wound healing. In male diabetic mice, this glucose oxidase/superoxide dismutase-integrated bacterial cellulose dressing (low endotoxin <0.1 EU/mL) accelerates wound closure to 92.1% by day 14, significantly outperforming the controls. Our strategy highlights a scalable and bio-orthogonal route for enhancing bacterial cellulose with user-defined bioactivities, thereby expanding its utility in advanced biomaterials development.
    DOI:  https://doi.org/10.1038/s41467-026-69130-8
  18. J Colloid Interface Sci. 2026 Jan 24. pii: S0021-9797(26)00153-0. [Epub ahead of print]709 139976
      Conductive hydrogels hold great potential for applications in flexible electronics due to their excellent flexibility and conductivity. However, hydrogels typically have poor mechanical properties and are prone to freezing and drying under extreme conditions, while a lack of shaping approaches severely restricts their capacity to form complex geometries and customized functionalities for high-performance devices. Herein, inspired by the synergistic mechanisms observed in arctic brown algae, we propose a novel strategy integrating ionic crosslinking, ion hydration, and hydrogen bonding networks for one-step photocurable 3D printing of organohydrogels. The bioinspired design of the organohydrogel provides remarkable tensile strength (4.65 MPa), excellent transparency (97%), high ionic conductivity (0.34 S/m), and ultrawide temperature tolerance (down to -50 °C), and is enabled by the synergistic mechanism to achieve rapid 3D printing (<2 s/100 μm). The 3D-printed organohydrogel is capable of versatile sensing platform of stress, motion, respiration, and temperature with exceptional adaptability. Customized cryo-tolerant sensors enable encrypted Morse code communication via strain-responsive signals at -50 °C. Utilizing inherent triboelectric and piezoresistive properties, we developed smart insoles with spatial pressure mapping for real-time gait and posture monitoring. This work lays the foundation for advanced human-machine interactions, soft robotics, customizable flexible electronics, and health monitors for applications in extreme environments.
    Keywords:  3D printing; Anti-drying; Anti-freezing; Flexible electronics; Organohydrogels
    DOI:  https://doi.org/10.1016/j.jcis.2026.139976
  19. ACS Sustain Chem Eng. 2026 Feb 02. 14(4): 1899-1910
      Chitin-rich mushroom-derived papers (MPs), processed from Agaricus bisporus pulps, offer a biobased route to sustainable materials. This work advances sustainable materials design by utilizing a renewable fungal feedstock, employing an additive-free aqueous pulping process, and enabling reconfiguration and reuse through reversible water-mediated bonding. MPs exhibit reversible and repeatable water-assisted reconfigurability and adhesion properties, enabled by both physical entanglement of mushroom-derived paper pulps (MPPs) and extensive hydrogen bonding and intermolecular interactions. Remarkably, these MPs demonstrate a high tensile strength of up to 14.94 ± 1.35 N/mm2 and an adhesion strength of up to 0.72 ± 0.13 N/mm2, with the repaired MPs retaining ∼70% of their original tensile strength. Proof-of-concept demonstrations showed the possible origami-like construction and multicycle reuse of MPs. These findings highlight MPs as reconfigurable and reusable paper-like materials, extending their lifecycle and reducing resource demand, thereby advancing sustainable materials engineering.
    Keywords:  cellulosic paper alternatives; mushroom-derived materials; reconfigurability; reusable papers; water-assisted adhesion
    DOI:  https://doi.org/10.1021/acssuschemeng.5c09559
  20. Macromol Biosci. 2026 Feb;26(2): e00400
      Hydrogels prepared from gelatin are ideal for mimicking the extracellular matrix (ECM) owing to their inherent cell-adhesive and protease-labile peptide sequences. While gelatin is highly water-soluble, it does not form the triple-helical structure. As a result, physically crosslinked gelatin-based hydrogels are only stable at low temperatures, precluding their use in 3D cell culture. Gelatin-methacryloyl (GelMA) and gelatin-norbornene (GelNB) have been developed to enable the stable crosslinking of gelatin-based hydrogels via chain-growth or step-growth photopolymerization. However, most gelatin-based hydrogels lack dynamically tunable properties unless macromers with dynamically crosslinkable motifs are used. Here, we integrate GelNB with dithiolane-containing crosslinker poly(ethylene glycol)-tetra-lipoic acid (PEG4LA)-for modular photo-crosslinking of GelNB into hydrogels under cytocompatible light exposure (365 nm, 5 mW/cm2) with a low photoinitiator concentration (1 mm LAP). Even under these mild reaction conditions, the stiffness of GelNB/PEG4LA hydrogels could be dynamically tuned by inducing dithiolane ring-opening via secondary light exposure, thereby creating dynamic and cytocompatible hydrogels suitable for in situ encapsulation, culture, and differentiation of human induced pluripotent stem cells (hiPSCs).
    Keywords:  3D/4D cell culture; dithiolanes; dynamic hydrogels; gelatin‐norbornene; induced pluripotent stem cells; lipoic acid
    DOI:  https://doi.org/10.1002/mabi.202500400
  21. Adv Sci (Weinh). 2026 Feb 06. e09313
      Bioprinting is a powerful tool for engineering living grafts, however replicating the composition, structure and function of native tissues remains a major challenge. During morphogenesis, cellular self-organization and matrix development are strongly influenced by the mechanical constraints provided by surrounding tissues, suggesting that such biophysical cues should be integrated into bioprinting strategies to engineer more biomimetic grafts. Here, we introduce a novel bioprinting platform that spatially patterns mesenchymal stem/stromal cell (MSC)-derived microtissues into mechanically tunable support baths. By modulating the bath's mechanical properties, we can precisely control the physical constraints applied post-printing, directing both filament geometry and cellular behavior. Support bath stiffness regulated mechano-sensitive gene expression and microtissue phenotype, with softer matrices favoring chondrogenesis and stiffer environments promoting (myo)fibrogenic differentiation. In addition, the physical properties of the non-degradable support bath modulated microtissue fusion and extracellular matrix organization, with increased collagen fiber alignment in stiffer baths. Leveraging these findings, it was possible to engineer either articular cartilage, meniscus, or ligament grafts with user-defined collagen architectures by simply varying the physical properties of the support bath. This platform establishes a foundation for bioprinting structurally anisotropic and phenotypically distinct constructs, thereby enabling the scalable engineering of a range of different musculoskeletal tissues.
    Keywords:  anisotropy; bioprinting; microtissues; stiffness; support bath
    DOI:  https://doi.org/10.1002/advs.202509313
  22. ACS Omega. 2026 Jan 27. 11(3): 3926-3936
      Combinatorial methods for preparing polymeric biomaterials enable the rapid identification of materials useful for many applications in science, medicine, and engineering. In the work described here, we demonstrate that side-chain reactive polymers can be used as templates for the rapid preparation of a small library of diversely functionalized protein-polymer conjugates. The activated ester polymer poly-(pentafluorophenyl acrylate) (PPFPA) was modified postpolymerization with substoichiometric equivalents of three hydrophilic primary amines to yield a library of amphiphilic, side-chain reactive copolymers. These copolymers were then conjugated to two receptor-targeting proteins, holotransferrin (hTF) and an engineered fibronectin-based protein (Fn3), through amine-activated ester coupling. We investigated the influence of polymer:protein ratio, side-chain chemistry (i.e., hydrophilic group identity and number of protein-reactive groups), and protein identity on conjugation efficiencies. Our results demonstrate that, for polymers of similar solubility in aqueous media, a larger polymer:protein ratio yields higher conjugation efficiencies. In addition, polymers with a greater number of reactive groups or shorter hydrophilic side chains improve protein conjugation efficiency. Finally, smaller proteins couple to the polymers more efficiently than do larger proteins. Collectively, the results described here demonstrate a modular approach for efficiently preparing bioconjugates with diverse chemistries that may be of interest in a broad range of applications.
    DOI:  https://doi.org/10.1021/acsomega.5c07215
  23. Biomater Transl. 2025 ;6(4): 437-449
      Three-dimensional (3D) cell culture systems provide a more physiological environment than traditional two-dimensional cultures by better mimicking the complex interactions within the extracellular matrix (ECM). Among the key properties of the ECM, viscoelasticity is essential for regulating cell behaviors, such as proliferation, differentiation, and migration. However, many present 3D culture systems are complex and technically demanding, which limits their broad application. In this study, we developed two hydrogel systems with identical stiffness but distinct viscoelastic properties, designed to serve as ECM-based 3D culture platforms. These hydrogels were constructed through the cross-linking reaction between type I collagen and functionalized polyethylene glycol derivatives, resulting in either reversible (dynamic) or stable (static) network structures. This platform effectively simulated ECM-like mechanical cues, enabling the investigation of viscoelastic effects on both neural and cancer cell responses. Our results demonstrated that dynamic hydrogels, characterized by rapid stress relaxation, enhanced PC12 cell elongation, promoted neural stem cell differentiation, and significantly facilitated the invasiveness and tumorigenic capacity of DU145 cells in vitro and in vivo. These findings highlight the critical importance of matrix viscoelasticity in modulating cell behavior and underscore the potential of this hydrogel-based system as a versatile and accessible tool for applications in neural tissue engineering, cancer research, and mechanobiology.
    Keywords:  3D cell culture; Cell behavior; Collagen; Hydrogel; Viscoelasticity
    DOI:  https://doi.org/10.12336/bmt.25.00096
  24. ACS Appl Mater Interfaces. 2026 Feb 06.
      Rapid and quantitative characterization of atomic defects in two-dimensional (2D) semiconductors and transistors is crucial for growth optimization and understanding of device behavior. However, such defect metrology remains challenging due to limitations of existing characterization methods, which are generally destructive and slow or lack the necessary sensitivity. Here, we use nondestructive lateral force microscopy (LFM) to directly map surface defects in monolayer WSe2 and WS2 on different growth substrates (SiO2 and sapphire), as well as in WSe2 transistors. Through LFM measurements on various WSe2 layers, we show that this technique can detect defect densities well below the range of typical Raman measurements on this material. We also demonstrate mapping of spatial variation of defect density within as-grown WSe2 and that the LFM technique can detect defects on suspended and polymer-supported monolayers, expanding the application space. Applied to WSe2 transistors, LFM uncovers defect densities over double that of similar as-grown films, suggesting that defects can be introduced by common fabrication processes. This work demonstrates the applications of LFM as a nondestructive defect characterization method for monitoring 2D material growth and device fabrication.
    Keywords:  2D materials; Raman spectroscopy; atomic force microscopy; defects; devices; transition metal dichalcogenides
    DOI:  https://doi.org/10.1021/acsami.5c19328
  25. Nat Commun. 2026 Feb 02.
      Shape-morphing mechanisms help organisms adapt to their environment and have inspired applications in advanced systems. Designing microrobots that are both miniaturized and capable of fast shape changes is challenging, as performance can be weakened at small scales. Ultrasound offers advantages such as fast response, repeatability, and programmability, making it suitable for enabling shape-morphing microrobots. Here, we introduce an acoustic micromachine composed of two microbubbles connected by a microhinge. Acoustic-field excitation generates interaction forces between the bubbles, enabling complete micromachine deformation within milliseconds. We also present design principles for programmable acoustic deformation, enabling both forward and inverse design, precise control, and information storage. By tuning the excitation amplitude, the micromachine can switch between multiple modes. As proof of concept, microlotus and microbird structures are demonstrated with controllable and stable performance.
    DOI:  https://doi.org/10.1038/s41467-026-68856-9
  26. ACS Appl Mater Interfaces. 2026 Feb 03.
      Despite their widespread application in automotive, maritime, and aerospace industries, conventional thermosets and their composites suffer from nonrecyclability. Current recyclable covalent adaptable networks offer a solution but are limited by poor heat resistance. Herein, we report a diphenyldisulfide-based polymer (BMS-DB) that overcomes this limitation. The BMS-DB polymers deliver an ultrahigh Tg of ∼275 °C (high storage modulus (E') of ∼410 MPa at 325 °C), robust mechanical properties, and rapid degradability. As a matrix for carbon fiber composites, it enables an unprecedented Tg of ∼305 °C and nondestructive recycling. This combination of ultrahigh-temperature capability and closed-loop recyclability makes these materials ideal candidates for severe-service environments such as aerospace.
    Keywords:  bismaleimide; composites; degradation; disulfide; ultrahigh-temperature
    DOI:  https://doi.org/10.1021/acsami.5c24646
  27. Nat Chem. 2026 Feb;18(2): 227-245
      Biological transcription uses dynamic machinery modulated by transcription factors and auxiliary environmental cues to control multiple biological processes. Misregulation of the transcription machinery leads to diverse genetic disorders and diseases. Here we discuss the application of DNA nanostructures and circuits in developing synthetic in vitro transcription frameworks that mimic dynamic features, such as switchable blockage of transcription by topological barriers, transcription machineries revealing transient dissipative kinetics, and bistable programs or oscillatory transcription circuits driven by feedback loops, paving the way to exploring and validating mechanisms in native transcription and their potential biological applications. Possible applications of the transcription frameworks for sensing, and future perspectives for autonomous therapeutics and the design of artificial cells, are discussed.
    DOI:  https://doi.org/10.1038/s41557-025-02046-w
  28. Adv Mater. 2026 Feb 07. e18168
      The design of high-sensitivity stretchable piezoelectric sensors remains challenging due to the inherent trade-off between the ability to achieve high levels of mechanical deformation while maintaining efficient stress transduction. Here, we propose a new topology-optimization strategy to construct stretchable piezoelectric sensors that efficiently utilize the spatial stress distribution and are able to adapt to a range of anisotropic mechanical stress states. By exploiting computer-aided topology optimization, the distribution of piezoelectric ceramic units within the sensor was tailored to maximize the degree of stress transfer, resulting in an increase of 103.5% and 59.7% in the maximum piezoelectric potential when subject to tension and torsion, respectively. To ensure structural stretchability and adaptability of the topology optimized sensors when subject to complex loading environments, a direct ink writing process was developed to create stretchable eutectic gallium-indium liquid alloy (EGaIn) electrodes. Based on a shear-driven mechanism of printing, new predictive theoretical equations governing printing performance were developed that could predict the printed state (with 94.7% accuracy) and enable trace width control (relative error < 15%). The final optimized sensor exhibited excellent sensitivity, achieving 14.0 V per strain and 0.10 V per degree when subject to tensile and torsional loads, exceeding the unoptimized device by 59.2% and 92.4%, respectively. Finally, inspired by the morphological characteristics of butterflies and guided by the topology-optimized layout, a multi-channel sensor was constructed to accurately identify the pattern and amplitude of a complex range of neck movements, demonstrating the significant potential of the new design and manufacturing approach for wearable electronics.
    Keywords:  liquid‐metal printing; motion monitoring; self‐powered sensor; stretchable piezoelectric sensors
    DOI:  https://doi.org/10.1002/adma.202518168
  29. Proc Natl Acad Sci U S A. 2026 Feb 03. 123(5): e2505183123
      An elastic spring network is an example of evolvable matter. It can be pruned to couple separated pairs of nodes so that when a strain is applied to one of them, the other responds either in-phase or out-of-phase. This produces two pruned networks, with incompatible functions, that are nearly identical but differ from each other by a set of "mutations" each of which removes or adds a single bond in the network. We generate ensembles of network pairs that differ by a fixed number, M, of discrete mutations and evaluate all M! mutational paths between the in- and out-of-phase behaviors up to M[Formula: see text] 14. With a threshold response for the network to be considered sufficiently fit for either function, so that nonfunctional networks are disallowed, only some mutational pathways are viable. We find that there is a surprisingly high critical response threshold above which no evolutionarily viable path exists between the two networks. The few remaining pathways at this critical value dictate much of the behavior along the evolutionary trajectory. The effect of multiple mutations is epistatic, that is, the impact of a mutation is not invariant but depends on what other mutations have already occurred. In most cases, the mutations break up into two distinct classes based on epistasis. The analysis clarifies how the number of mutations and the position of a mutation along the pathway affect the evolutionary outcome.
    Keywords:  epistasis; evolution; fitness landscape; function switch; mechanical network
    DOI:  https://doi.org/10.1073/pnas.2505183123
  30. Adv Mater. 2026 Feb 04. e14781
      Artificial muscles are essential components in the advancement of next-generation soft robotics, biomedical devices, and adaptive wearables. While conventional fiber-based actuators often rely on multi-material assemblies and complex interfacial engineering, their performance is limited by structural heterogeneity and low-efficient energy coupling. This review highlights the emerging paradigm of single-fiber or in-fiber artificial muscle design, where actuation functionality is intrinsically encoded within the molecular architecture of individual fibers. We comprehensively examine state-of-the-art material systems such as phase-transition materials, block copolymer self-assemblies, mechanically interlocked polymers, covalent supramolecular hybrids, and woven polymer networks. Particular emphasis is placed on the structure-property-function relationships that govern the actuation strain, stress output, response speed, and long-term durability. We also propose a unified framework for evaluating single fiber actuator performance based on key metrics and critically discuss manufacturing challenges, scalability, and integration with smart sensing system. This review provides a roadmap for molecular design of the high-performance artificial muscle, offering new strategies for intelligent actuation and soft material systems in real-world applications.
    Keywords:  artificial muscles; molecular design; performance breakthrough; single‐fiber or in‐fiber
    DOI:  https://doi.org/10.1002/adma.202514781
  31. Biomacromolecules. 2026 Feb 03.
      The development of rapid, chemoselective covalent bond-forming reactions enables the assembly of hydrogel scaffolds suitable for applications in cellular encapsulation. We previously reported that amide-forming ligations between potassium acyltrifluoroborates (KATs) and hydroxylamines produce robust hydrogels that have excellent cytocompatibility, but the requirement for somewhat acidic conditions for efficient hydrogel assemblies limited their application to robust cell types. To overcome this constraint, we have recently found that quinolinium acyltrifluoroborates (QATs) serve as highly efficient reaction partners for amide-forming reactions at neutral pH. In this article, we document the construction of poly(ethylene glycol) (PEG)-derived hydrogels by efficient cross-linking of QAT-functionalized macromers with a partner hydroxylamine-functionalized macromer. Gelation occurs at physiological pH in under 2 min, offering a rapid and facile approach to the immobilization of delicate stromal cells. The cytocompatibility of the cross-linking was demonstrated by in situ gelation in the presence of human mesenchymal stem cells and sustained cell viability for 7 days. Facile incorporation of a cyclic cell adhesion peptide, simply by including the reaction partner in the gelation reactions, illustrated that the desired components can be introduced into the gels without further elaboration.
    DOI:  https://doi.org/10.1021/acs.biomac.5c02082
  32. Nat Commun. 2026 Feb 06.
      Hydrogel coatings are a promising strategy to reduce friction and wear in biomedical implants, yet replicating the durability of natural cartilage remains a key challenge due to the inherent trade-off between low friction and high wear resistance. Here, we present a picot-fiber hydrogel coating (PFHC) that mimics the hierarchical architecture of cartilage by integrating a lubricious surface layer with a tough, fiber-reinforced core layer. The picot fibers, formed by folded peptide strands with hidden loops, endow the core layer with efficient load-bearing capacity, while the loosely packed, open-structured top layer preserves hydration lubrication. The resulting PFHC achieves both ultralow friction (~0.009) and high wear resistance under long-term sliding over 100,000 cycles, comparable to natural cartilage. By decoupling lubrication and load-bearing functionalities across distinct structural layers, PFHC overcomes the conventional limitations of hydrogel coatings, providing a generalizable strategy to integrate lubrication and mechanical durability for reliable, long-lasting implant interfaces.
    DOI:  https://doi.org/10.1038/s41467-026-69322-2
  33. Nanoscale. 2026 Feb 06.
      Conductive hydrogels, combining flexibility and electrical conductivity, show great potential in flexible electronics, wearable sensors, and smart materials. However, their practical applications remain constrained by insufficient mechanical strength, unstable sensing performance, and low structural integration. To address these challenges, we develop a highly sensitive MXene@PDA/PF127-DA/Zn2+ conductive hydrogel, which achieves an effective balance between mechanical strength and sensing performance through the synergistic effect of multiple components. MXene nanosheets serve as the primary conductive framework, while the polydopamine coating effectively enhances its dispersibility and interfacial adhesion. In addition, the double-bond modified PF127-DA can self-assemble into micelles, providing a dynamic structure that offers better elastic properties for the conductive hydrogel. Finally, the introduction of Zn2+ as a dynamic coordination crosslinker further enhances mechanical toughness. This collaborative design makes it possible to construct a new type of conductive hydrogel system with high mechanical strength, excellent stability, and tunable sensing performance. When attached to human skin, the conductive hydrogel can quickly respond (response time up to 0.089 s) and accurately detect subtle electrical signals associated with joint motion and muscle contraction. Furthermore, real-time signal acquisition and wireless transmission are achieved through an integrated electrochemical workstation and Bluetooth module, enabling efficient motion monitoring. This study provides a promising strategy for designing multifunctional conductive hydrogels for next-generation wearable bioelectronic devices.
    DOI:  https://doi.org/10.1039/d5nr04391c
  34. ACS Nano. 2026 Feb 05.
      The many surface proteins on extracellular vesicles (EVs) allow them to target recipient cells and modulate cellular responses. Despite their importance, relating surface protein and EV function is challenging due to surface protein heterogeneity. Here, we create a bottom-up, cell-free protein-synthesis platform to engineer artificial nanovesicles (ANVs) that display different EV surface protein domains. The platform is termed VESSEL (Vesicle Engineering Systems using Synthetic Expression and Loading). The surface proteins are selected based on proteomics data of native EVs from placental mesenchymal stem cells (PMSCs). To create VESSEL, we establish a protein anchor based on the bacteria membrane protein Aquaporin-Z. This anchor allows the flexible and cell-free protein synthesis of 39 different EV surface protein domains, each anchoring into more than 108 ANVs per μL. Furthermore, we measure the ANVs using high-fidelity assays, including single-ANV flow cytometry, super-resolution imaging, and vesicle-based ELISA. Next, we show the impact of each EV surface protein on cellular uptake. Specifically, we find that certain EV surface protein domains govern ANV uptake into HEK293FT cells, explaining the variable observations in the field. We discovered new proteins, such as CADM1 and NPTN, that mediate high-efficiency cellular uptake. Additionally, five proteins were selected for our neuroprotection assay, where three proteins were significant in increasing SH-SY5Y neurite growth. Our work demonstrates a high-throughput cell-free synthesis platform for studying surface proteins of EVs. It enables the systematic interrogation of EV's function as "signalosomes" and facilitates the designing of well-defined EV mimetics to mediate cellular function.
    Keywords:  Cell-Free Protein Synthesis; Cellular Uptake; Nanovesicle; Neuroprotection; Protein Anchor
    DOI:  https://doi.org/10.1021/acsnano.5c05047
  35. Nat Comput Sci. 2026 Feb 02.
      The synthesis of crystalline materials, such as zeolites, remains a notable challenge owing to a high-dimensional synthesis space, intricate structure-synthesis relationships and time-consuming experiments. Here, considering the 'one-to-many' relationship between structure and synthesis, we propose DiffSyn, a generative diffusion model trained on over 23,000 synthesis recipes that span 50 years of literature. DiffSyn generates probable synthesis routes conditioned on a desired zeolite structure and an organic template. DiffSyn achieves state-of-the-art performance by capturing the multi-modal nature of structure-synthesis relationships. We apply DiffSyn to differentiate among competing phases and generate optimal synthesis routes. As a proof of concept, we synthesize a UFI material using DiffSyn-generated synthesis routes. These routes, rationalized by density functional theory binding energies, resulted in the successful synthesis of a UFI material with a high Si/AlICP of 19.0, which is expected to improve thermal stability.
    DOI:  https://doi.org/10.1038/s43588-025-00949-9
  36. Lab Chip. 2026 Feb 04.
      Bacteria in surface-attached communities often engage in social interactions with neighbouring microbes. Spatial structure within these communities is thought to strongly influence these interactions, yet there is a significant lack of experimental platforms which allow for the tight spatial control of microbial interactions at the microscale, severely limiting our ability to investigate the relationship between spatial structure and community development. Here, we demonstrate a workflow for patterning and growing two bacterial species on a template with high throughput (∼105 patterned cells per template) and micron-scale precision. We demonstrate a methodology for directional sequential capillary assembly of colloidal particles in combination with nanobody-functionalised particles that enable highly specific, bio-orthogonal binding reactions between bacteria and surface deposited particles. Using Staphylococcus aureus and Escherichia coli as model systems, we demonstrate how these organisms can be patterned in any desired spatial configuration where resulting communal growth can be monitored under the microscope. This technique enables careful investigations into the role of initial spatial structure on microbial interactions at low cell density, which is crucial to understanding and manipulating microbial community development.
    DOI:  https://doi.org/10.1039/d6lc00040a
  37. Angew Chem Int Ed Engl. 2026 Feb 01. e22618
      Polyethylene (PE) materials are indispensable to modern infrastructure due to their exceptional thermal, mechanical, and chemical resilience. However, the same properties that make these materials durable also render them environmentally persistent and unrecyclable by conventional means, posing a critical sustainability challenge. Here, we report a mechanochemically triggered, chemically recyclable PE-like system that enables the closed-loop recycling of cross-linked polyethylene (XLPE). Through palladium-catalyzed coordination copolymerization of ethylene with the cyclobutene-fused ester (CBE) comonomer, polar PE-like materials with tunable properties are achieved. Upon optimal mechanical activation in the presence of a radical inhibitor, the CBE units undergo ring opening, installing ester linkages into the polymer backbone. Notably, the high crystallinity of copolymers with low CBE content enables ball-milling to achieve activation efficiency comparable to cryo-milling. Subsequent ethanolysis of ester linkages cleanly converts the initial copolymer into multifunctional oligomers, which can be repolymerized after hydrogenation via transesterification to yield a recyclable XLPE with properties comparable to a commercial analogue. This work demonstrates a robust platform for reconciling the durability and recyclability of polyethylene, offering a transformative route toward sustainable polyolefins.
    Keywords:  ball mill; cross‐linked PE; polyethylene recycling; polymer degradation; polymer mechanochemistry
    DOI:  https://doi.org/10.1002/anie.202522618
  38. Chem Rev. 2026 Feb 02.
      Mechanochemistry has emerged as a powerful strategy for controlling chemical reactivity and tuning material properties through applied force. Within this growing field, mechanochromic mechanophores have attracted particular attention as versatile molecular probes that transduce mechanical inputs into optical signals via force-induced structural transformations. By enabling direct visualization of stress and damage in real time, these systems provide unique opportunities for applications in stress sensing, damage detection, and the design of adaptive materials. This Review offers a systematic framework of classifying mechanochromic mechanophores, with a focus on their molecular design principles and activation mechanisms. We highlight how methods of force application and characterization critically shape the study of mechanochromism and examine how mechanophore scaffolds, polymer architectures, and environmental factors collectively dictate optical responses. Applications are critically assessed to underscore the role of mechanochromic systems as a bridge between fundamental mechanochemistry and functional materials engineering. Finally, we outline current challenges and emerging opportunities, with particular emphasis on the growing potential of mechanochromic systems in biological and biomedical contexts.
    DOI:  https://doi.org/10.1021/acs.chemrev.5c00789
  39. Biofabrication. 2026 Feb 05.
      Force generation dynamics in native muscle tissues have been stringently optimized by evolution. Realizing similar contractile dynamics in a widely available biomaterial and subsequently fabricating macroscopic functional modules from them remains challenging. Herein, we tailor two-photon stereolithography to 3D print synthetic muscles made from bovine serum albumin to realize 1 mm long contractile fibers. We show that pH-dependent contractions in these synthetic muscles follow parabolic force-length relationships similar to biological muscles. Achieved stress outputs of 0.78 ± 0.13 N/cm2 were within an order of magnitude of smooth and cardiac muscle. Stretch-shortening work loops performed under different strain rates in turn revealed a viscoelastic behavior and significant velocity dependence of work and net power, more similar to skeletal muscle. That an isotropic protein hydrogel can achieve such dynamics, reinforces the notion that these are not limited to sarcomere-level ordering and suggests a more general design space for non-canonical conformational dynamics to engineer performance improvements in artificial muscle materials.
    Keywords:  bovine serum albumin (BSA); force-length relationship (FLR); hydrogels; micromechanics; synthetic muscle; two-photon polymerization; work loops
    DOI:  https://doi.org/10.1088/1758-5090/ae4272
  40. ACS Omega. 2026 Jan 27. 11(3): 4362-4398
      Aragonite-proteinoid microstructures are an emerging type of biocomputing material. They mix inorganic calcium carbonate with self-assembled organic proteinoid networks. Scanning electron microscopy shows a range of structures. These include isolated microspheres and complex networks over 50 μm. They have dendritic shapes, with uneven nodes that create linear patterns resembling simple network topologies. Electrochemical testing shows a threshold response. This allows for all seven basic Boolean logic operations: AND, OR, NOT, NAND, NOR, XOR, and XNOR. It does this by classifying analog signals into binary states. This suggests a promising future for material-based computation. Frequency-dependent square wave voltammetry shows power-law scaling. It performs best in the 30-50 Hz range, which is important for biological use. This indicates adjustable electrochemical properties that are ideal for bioelectronic applications. The systems show autonomous oscillatory behavior for over 25 h. They maintain a steady ultralow frequency, like biological rhythms. This means they generate signals on their own, without any outside help. Impedance spectroscopy shows stable circuit features. There are strong links between resistive and capacitive parts. However, cyclic voltammetry shows that electrochemical degradation increases over time. These findings show that aragonite-proteinoid microstructures are well-suited for novel computing uses. They can help with things like autonomous sensing, neuromorphic devices, and biohybrid electronics. These microstructures use mineral-organic interfaces for processing information and generating signals. This approach connects synthetic materials to biological computing principles.
    DOI:  https://doi.org/10.1021/acsomega.5c09786
  41. bioRxiv. 2026 Jan 22. pii: 2026.01.19.700377. [Epub ahead of print]
      Electrospun fiber meshes have long served as biomaterials in a wide range of biomedical applications due to their functional similarities to extracellular matrix and highly tunable properties. Altering the mechanical behaviors of individual fibers and their microarchitecture (e.g.; diameter, crimp, orientation, density) can in principle be used to control bulk level behaviors. Moreover, electrospun meshes are often combined with softer coatings and hydrogels to control surface interactions with body tissues. Yet, fully optimizing their behaviors for specific applications remains an elusive target due to a continued lack of understanding of the micromechanical mechanisms and their relation to bulk mechanical behaviors. Our goal herein was to understand how actual nanoCT-generated 3D microfiber geometry can be used to predict bulk mechanical properties of hydrogel-mesh composites. Electrospun polyurethane meshes were fabricated with a random fiber orientation and coated with a PEG-based hydrogel. The fiber-hydrogel composite was then imaged with a nanoCT scanner at a voxel resolution of 180 nm. From these images, custom Python programs were written to segment, refine, and tesselate a high-resolution finite element of the fiber mesh and hydrogel volumes into a single integrated bi-material finite element model. The resulting mesh was used to run simulations of the planar biaxial mechanical tests used to characterize the bulk mechanical behaviors. Our framework thus enabled systematic investigations of both the macroscopic bulk mechanical response of the overall fiber mesh and the microscopic localized mechanical response of fibers under various stages of loading. The resultant simulations were accurate and predictive of the bulk mechanical responses. It is interesting to note that the fiber-hydrogel composite material experienced the largest stresses within the fiber phase and the largest strains within the hydrogel. This key result underscores that while the previous analytical model assumed local affine deformations, at the microscale this assumption does not hold. We also found very different effective fiber stress-strain responses in each model. It is likely these differences are due to the substantial heterogeneous non-affine local deformations present in the actual fiber-hydrogel composite. This finding further reveals the need for more rigorous approaches to better understand how electrospun-based materials function in order to improve their use in modern medical devices and implants.
    DOI:  https://doi.org/10.64898/2026.01.19.700377
  42. Small. 2026 Feb 01. e12610
      Reconstitution of synthetic cells holds potential to advance synthetic biology, biomanufacturing, and therapeutics. Microfluidic generation of cell-sized liposomes via double emulsion templating offers precise control over composition and formation process, yet the principles underlying solvent-mediated dewetting remain poorly understood. Using a solvent combination of hexanol and paraffin oil, we demonstrate that solvent-mediated dewetting liposome generation entails both solvent removal and the application of mechanical stimuli. Solvent removal suffices to induce the morphological transition from double emulsions to partially dewetted liposomes exhibiting low and high budding angles of the residual oil pockets. This transition is driven by relaxation of monolayer and membrane tensions, arising from the increased lipid packing density at the liposome interfaces during solvent depletion. While dewetting kinetics and intermediate stages are governed by solvent removal rate, complete dewetting is not spontaneous. Using optical tweezers, we identify tethering between the liposome and oil pocket and characterize the mechanical force required for liposome detachment. By integrating these principles, a predictive, high-throughput approach for generating biocompatible, surfactant-free liposomes is provided. These findings establish a mechanistic framework for liposome dewetting and, through similarities to lipid droplet morphogenesis, offer a protocell platform that could further the understanding of biological budding processes.
    Keywords:  cell‐sized liposome; giant unilamellar vesicle; microfluidic liposome; solvent‐mediated dewetting; synthetic cell
    DOI:  https://doi.org/10.1002/smll.202512610
  43. Carbohydr Polym. 2026 Apr 01. pii: S0144-8617(25)01649-2. [Epub ahead of print]377 124865
      Hydrogels have gained prominence as a distinctive material for smart healthcare devices and wearable electronics, owing to their inherent biocompatibility, tissue-like mechanical properties and superior water and oxygen permeability. In most application scenarios, hydrogels are required to have certain adhesion properties. However, current hydrogel adhesive systems are fundamentally constrained by two intrinsic material restrictions: low mechanical strength, and compromised adhesion in humid environments (under conditions such as sweating and bleeding), which severely restrict their practical applications. Inspired by the covalent and physical synergistic adhesion strategy of mussels and the interfacial water-capturing mechanism of spiderwebs, we designed and fabricated an ultrathin poly (acrylic acid) (PAA)/N-hydroxysuccinimide (NHS)/cellulose nanocrystal (CNC) composite hydrogel (PNCGels) tape. This PNCGels tape adopts a dry crosslinking mode, which enables the carboxyl groups in the hydrogel rapidly form physical interactions such as hydrogen bonds with tissue surface within less than 5 s. Subsequently, the NHS ester groups grafted onto PAA couple covalently with primary amine groups in the tissue. Additionally, the CNC nanofillers enhance cohesive strength by reconstructing a dynamic hydrogen-bonding network to balance adhesion, which ensures stable adhesion even in the presence of sweat. The resultant PNCGels tape displayed remarkable wearability (thickness of only 250 μm), excellent adhesive properties (adhesion strength: 128 kPa), good mechanical performance (tensile strength: 1400 kPa). The integrated features of ultra-slimness, high toughness, and humidity-resistant adhesion provide a transformative platform for advanced applications, including smart wound dressings (hemostasis, on-demand removal) and motion sensing technologies.
    Keywords:  Adhesion; Hydrogel tape; Nanocellulose; Sweat adaptation
    DOI:  https://doi.org/10.1016/j.carbpol.2025.124865
  44. Proc Natl Acad Sci U S A. 2026 Feb 10. 123(6): e2529275123
      The organizational complexity of biominerals has long fascinated scientists seeking to understand biological programming and implement new developments in biomimetic materials chemistry. Nonclassical crystallization pathways have been observed and analyzed in typical crystalline biominerals, such as calcium phosphate, calcium carbonate, and ferric oxide, involving the controlled attachment and reconfiguration of nanoparticles and clusters on organic templates. However, the understanding of templated amorphous silica mineralization remains limited, hindering the rational design of complex silica-based materials. Here, we report the finding of ultrastable and monodispersed cationic silica cluster (CSC) and their assembly using DNA nanostructures as programmable attachment templates. Cryo-EM imaging reveal that a typical CSC with a diameter of gyration of ~3.9 nm and an average molecular weight of ~8, 262 Da is characteristic of a branched hierarchical structure. We demonstrate high-fidelity silicification by tuning the composition and structure of CSC, providing a unified model of silicification by cluster attachment. Our findings pave the way toward the molecular tuning of pre- and postnucleation stages of sol-gel reactions and provide insights for the design of silica-based materials with controlled organization and functionality.
    Keywords:  DNA nanotechnology; amorphous silica; biominerals
    DOI:  https://doi.org/10.1073/pnas.2529275123
  45. Nat Commun. 2026 Jan 30.
      Stem cell differentiation dynamically remodels and stiffens the extracellular matrix (ECM), generating stage-specific biomechanical cues that guide tissue development. However, conventional biomaterials, designed to mimic mature ECM stiffness, neglect its spatiotemporal heterogeneity due to their static, non-evolvable nature. Herein, we develop a cell-programmed adaptative contraction (CPAC) hydrogel that enables mesenchymal stem cells (MSCs) to actively remodel their microenvironment through alkaline phosphatase (an early osteogenic marker)-mediated hydrophilic-to-hydrophobic transition and contraction of microgels. This cell-programmed remodeling establishes local mechanical heterogeneity and promotes osteogenesis through a positive feedback loop. Mechanistically, the evolving matrix enhances mechanotransduction-related microRNA expression, suppresses EZH2, and reduces H3K27 trimethylation to active osteogenic transcription. In vivo, MSC-laden CPAC hydrogels significantly enhance the repair of rat cranial defects. These findings introduce a paradigm of cell-instructed, dynamically evolvable biomaterials that recapitulate the adaptive nature of native ECM to orchestrate stem cell fate and tissue morphogenesis.
    DOI:  https://doi.org/10.1038/s41467-026-69004-z
  46. J Mater Chem B. 2026 Feb 02.
      Conductive hydrogels are promising candidates for neural bioelectrodes due to their softness, ionic permeability, and reduced mechanical mismatch with neural tissue. However, pristine biopolymer matrices such as alginate-gelatin (Alg-GEL) lack sufficient electrical functionality. Here, Alg-GEL hydrogels incorporating PEDOT:PSS, polypyrrole (PPy/PSS), or both were developed via blending and in situ polymerization, yielding a tunable family of soft, electroactive materials. The hydrogels exhibited Young's moduli of 5-70 kPa, depending on polymer loading, while electrical conductivities ranged from 0.1 to 3.7 S cm-1, with the highest values observed in PEDOT-PPy hybrids. Electrochemical measurements showed impedance values of 380-830 Ω cm2 at 1 kHz, an electrochemical stability window of approximately -0.85 to +1.2 V vs. Ag/AgClsat, and current injection limits reaching 4 mA, comparable to platinum electrodes. Swelling studies indicated that PEDOT-modified hydrogels achieved 41-56% swelling after 24 hours. PPy-based hydrogels swelled to approximately 97% and hybrid systems showed behavior dependent on their composition. All conductive formulations demonstrated improved long-term stability compared to pristine Alg-GEL, which gradually lost mass over 28 days of incubation. In contrast, hydrogels containing PEDOT and PPy maintained nearly constant wet weight, consistent with the formation of interpenetrating networks that prevented polymer degradation and leaching. Biological evaluation with NIH3T3 fibroblasts showed that all hydrogels were cytocompatible. PPy-only and PPy-PEDOT hybrids supported higher metabolic activity and more attached and spread cells after 7 days compared to Alg-GEL, while PEDOT-only samples showed similar or slightly reduced cell activity. These results confirm excellent cytocompatibility and suggest that PPy-rich domains improve cell-material interactions. Overall, PEDOT- and PPy-modified Alg-GEL hydrogels offer high conductivity, softness, electrochemical stability, long-term durability, and biocompatibility, creating a versatile and adjustable platform for next-generation soft neural interfaces.
    DOI:  https://doi.org/10.1039/d5tb02148k
  47. bioRxiv. 2026 Jan 21. pii: 2026.01.16.699961. [Epub ahead of print]
      Chronic exposure to inorganic arsenic remains a major global health concern, as arsenite is frequently present in contaminated food and drinking water and readily absorbed through the gastrointestinal (GI) tract. Once internalized, arsenite accumulates in tissues and contributes to long-term health effects, including cancer, organ dysfunction, and neurological disorders. Despite extensive efforts to reduce environmental contamination, there are currently no practical strategies to prevent dietary arsenite from entering the human body during digestion. Here, we report a synthetic biology-based approach that uses engineered probiotics to detect and sequester arsenite directly within the GI tract before systemic absorption occurs. We engineered Escherichia coli Nissle 1917 (EcN), a probiotic strain, to function as a living arsenite-interception system. Central to this design is an arsenite-responsive genetic toggle switch that activates chelator expression upon exposure and sustains production under biostatic conditions, while automatically shutting off during active cell division to limit metabolic burden and enhance biosafety. In parallel, we engineered an arsenite-binding protein derived from the transcriptional regulator ArsR to eliminate DNA-binding activity while retaining high-affinity metal binding, yielding a non-toxic chelator suitable for intracellular sequestration. The resulting engineered strain efficiently removed arsenite from its surrounding environment in vitro while maintaining robust cell viability and growth. To translate these findings to an in vivo context, we developed a mass-transfer model describing arsenite distribution among the stomach lumen, engineered bacteria, and epithelial cells. This model guided the selection of a bacterial dose predicted to substantially deplete lumenal arsenite prior to epithelial uptake. Using this strategy, we demonstrated in a mouse GI model that oral administration of engineered EcN markedly reduced arsenite entry into the bloodstream compared with wild-type EcN or no-bacteria controls. Together, these results establish a programmable probiotic platform for intercepting dietary arsenite and highlight a potential strategy for preventing absorption of environmental toxicants using living microbial therapeutics.
    DOI:  https://doi.org/10.64898/2026.01.16.699961
  48. Mater Today Bio. 2026 Apr;37 102842
      Damage or functional failure of vital organs remains a major clinical challenge, while the availability of donor organs for transplantation is severely limited. As a result, tissue engineering has emerged as a promising strategy for organ replacement; however, conventional top-down tissue engineering, which employs scaffolds to provide three-dimensional growth environments, cannot ensure precise cell positioning, restricting its applicability to complex and heterogeneous tissues. In contrast, bottom-up strategies that assemble spheroids or organoids as modular building blocks offer a more effective route to organ-like constructs. Nevertheless, they suffer from low reproducibility because of spontaneous cell self-assembly. Three-dimensional bioprinting provides a promising solution for the reproducible fabrication of multicellular organ building blocks (OBBs). At the same time, while extrusion-based bioprinting offers high reproducibility, its limited dimensional accuracy has restricted its use for fabricating OBBs that require both precise microarchitectures and reliable assembly. Here, we address this limitation by introducing a strategy in which bioinks are directly bioprinted within three-dimensionally printed molds, enabling the formation of OBBs with well-defined geometries and controlled spatial organization. By combining mold-guided bioprinting with multimaterial preset extrusion, we demonstrated the fabrication of heterogeneous OBBs with microscale architectures while preserving the modularity essential for bottom-up assembly. This approach resolves the conventional trade-off between structural precision and assembly-based scalability, allowing the construction of large tissue constructs with hierarchical vascular networks. Overall, this work presents a 3D bioprinting-based OBB fabrication strategy that integrates precision manufacturing with bottom-up tissue assembly, offering a reproducible and scalable framework for bioartificial organ engineering.
    Keywords:  3D bioprinting; Bioartificial organ; Hierarchical vascularized tissue; Organ building block (OBB); Preset extrusion bioprinting
    DOI:  https://doi.org/10.1016/j.mtbio.2026.102842
  49. Nat Rev Mater. 2026 Jan;11(1): 26-49
      Historic and ongoing efforts in ecology and environmental science have highlighted the pressing need to monitor the health, sustainability, and productivity of global and local ecosystems. Interest in these areas reflects a need to both determine the suitability of environments to support human activity (settlement, agriculture, industry) and to evaluate the impacts of such anthropogenic action. Of interest are chemical, biological, and physical factors which reduce ecosystem viability due to human intervention. Evaluating these factors, and their impact on global health, ecological stability, and resource availability demands improvements to existing environmental sensing technologies. Current methods to quantify chemical pollutants, biological factors, and deleterious physical conditions affecting target ecosystems suffer from lack of automation and narrow spatiotemporal range. Recent advances in materials science, chemistry, electronics, and robotics offer solutions to this problem. A vision emerges for fully autonomous, networked, and ecoresorbable sensing systems that can be deployed over large aerial, terrestrial, and aquatic environments. This Review describes ongoing efforts in these areas, focusing on materials advances supporting the accurate quantification of environmental factors with apparatus that accommodates full or partial device resorption. Discussion begins with an overview of hazards affecting global ecosystems, followed by a description of existing detection methods to quantify their severity. We proceed with an exploration of existing and developing technologies affecting sensor dispersion, motility, communication, and power. Finally, we describe exciting recent efforts in the development of environmentally degradable materials that could prove beneficial in the realization of massively distributed (millions of individual sensors) transient sensor networks.
    DOI:  https://doi.org/10.1038/s41578-025-00838-7
  50. Cell. 2026 Feb 05. pii: S0092-8674(25)01490-4. [Epub ahead of print]
      Why symbiotic organisms evolve irreversible dependencies on hosts is an outstanding question. We report a biological stealth device in a beetle that permits infiltration of ant societies. Via transcriptional silencing, the beetle switches off biosynthesis of cuticular hydrocarbons (CHCs)-body surface pheromones that function pleiotropically as a waxy desiccation barrier. Silencing transforms the beetle into a chemical blank slate onto which ant CHCs are transferred via grooming behavior, leading to perfect chemical mimicry and acceptance into the colony. Silencing is irreversible, however, forcing the beetle into a chronic dependence on ants to both maintain mimicry and prevent desiccation. We show that evolutionary reversion of the silencing mechanism would render the beetle detectable to ants; conversely, reversion of the beetle's attraction to ants would render it desiccation prone. Symbiotic entrenchment can thus arise from epistasis between symbiotic traits, locking lineages into a Catch-22 that obstructs reversion to living freely.
    Keywords:  ants; behavior; biosynthesis; cell biology; chemical ecology; evolution; irreversibility; obligate symbiosis; rove beetles
    DOI:  https://doi.org/10.1016/j.cell.2025.12.041
  51. ACS Nano. 2026 Feb 03.
      Bioinspired nanofluidic systems that utilize ions as signal carriers hold great promise for emulating neural processing in biochemical sensing and neuromorphic computing. However, achieving parallel, brain-like processing of multiple biochemical signals remains a significant challenge. Herein, we present a nanofluidic artificial postsynaptic membrane (APM) functionalized with confined DNA aptamers to construct a neuromorphic signal processing platform. Target-induced conformational switching of DNA aptamers dynamically modulates ionic transport through nanochannels, effectively mimicking synaptic information transmission. The integration of cross-responsive aptamer-based APM units into a cascaded logic system enables signal processing without relying on the physical series network of nanochannels. By independently addressing and reading each unit, dendritic multi-input integration and brain-like information fusion are achieved at the signal-algorithm level, and 100% accurate discrimination of multiple targets is reached. This approach marks a conceptual shift from the traditional "one-probe-one-target" model toward a brain-inspired, multitarget recognition architecture. The fusion of DNA probes with nanofluidic logic and their cascade at the signal level enables the development of neuromorphic biochips with integrated processing capabilities for multiplexed signals.
    Keywords:  DNA aptamers; artificial postsynaptic membrane; cascade signal; nanofluidics; neuromorphic discrimination
    DOI:  https://doi.org/10.1021/acsnano.5c16862
  52. ACS Sens. 2026 Feb 06. XXX
      The development of systems capable of dynamic, on-demand target detection marks a transformative advance for biomanufacturing, diagnostics, and environmental monitoring. Nanobodies can recognize targets ranging from bacteria and proteins to small molecules, yet conventional platforms remain static, with perpetually exposed binding sites that hinder control in dynamic settings. We introduce an adaptive, on-demand sensing nanobody platform that leverages electrically responsive oligopeptides to gate antigen-binding sites between OFF (shielded) and ON (exposed) states. Upon electrical activation, binding sites are revealed with >80% efficiency, enabling real-time, selective detection. The system achieves pg/mL sensitivity over a broad dynamic range (pg/mL-μg/mL), performs reliably in serum and cell culture media, and supports multiplexed detection via independently addressable electrodes. This electroresponsive architecture defines a new paradigm for programmable, high-performance molecular detection.
    Keywords:  dynamic systems; electro-activation; nanobody; on-demand sensing; responsive peptides; self-assembled monolayers
    DOI:  https://doi.org/10.1021/acssensors.5c04140
  53. ACS Appl Mater Interfaces. 2026 Feb 06.
      Phospholipids are biocompatible and versatile materials commonly used in the design of nanodelivery systems. However, the relationship between the structural characteristics of phospholipids and the physiological behavior of lipid-based nanoparticles remains inadequately understood. To explore the key structural features influencing efficient and targeted delivery, we created a library of phospholipid-coated gold nanoparticles (Lip@AuNPs), comprising 12 distinct formulations. These nanoparticles varied systematically in their headgroups (PA, PS, PC, and PE) and aliphatic chain lengths (6:0, 12:0, and 18:0). We investigated their effects on protein adsorption, cellular uptake, and in vivo delivery. Our findings showed that phospholipids with zwitterionic headgroups (PC and PE) reduced complement protein adsorption, enhanced selective uptake by nonphagocytic cells, and promoted increased accumulation in the spleen. Conversely, AuNPs coated with phospholipids containing shorter aliphatic chains exhibited higher serum protein adsorption, resulting in decreased and nonselective cellular uptake, which extended the circulation time of the nanoparticles in the bloodstream. This combinatorial approach provides valuable insights into the role of the phospholipid structure in nanoparticle design and offers practical guidance for developing lipid-based delivery systems with improved targeting and therapeutic efficacy.
    Keywords:  biodistribution; intracellular delivery; nanocombinatorial chemistry; phospholipids; protein corona
    DOI:  https://doi.org/10.1021/acsami.5c21974