bims-fagtap Biomed News
on Phage therapies and applications
Issue of 2026–05–17
thirty-six papers selected by
Luca Bolliger, lxBio



  1. Phage (New Rochelle). 2026 Mar;7(1): 51-61
      Bacteriophage therapy, which employs bacteriophages to eliminate bacteria, is gaining attention as a promising alternative to antibiotics amid mounting antimicrobial resistance. It has shown potential in treating chronic, multidrug-resistant infections, especially in vulnerable populations such as transplant patients. Moreover, advances in genomics, synthetic biology, and CRISPR-associated protein 9 have enabled the engineering of phages with broader host ranges and improved safety, while tools such as phage libraries and innovative delivery systems have enhanced its therapeutic effectiveness. Personalized phage therapy, guided by individual microbiota profiles, has demonstrated encouraging clinical outcomes as well. However, challenges remain, including regulatory barriers, safety concerns, and the emergence of phage-resistant bacteria. In response, continued research is essential to optimize treatment protocols and validate their clinical efficacy in standardized trials. Future priorities should include refining phage engineering, building robust regulatory frameworks, and establishing clear clinical guidelines. With coordinated global efforts, phage therapy could become a powerful, widely accessible tool in the ongoing battle against antibiotic-resistant infections.
    Keywords:  antibiotic resistance; bacteriophage; multidrug-resistant infection; personalized medicine; phage therapy
    DOI:  https://doi.org/10.1177/26416549251409584
  2. Cell Host Microbe. 2026 May 13. pii: S1931-3128(26)00168-X. [Epub ahead of print]34(5): 761-765
      Gut bacteriophages defy simple classification as beneficial or harmful, instead shifting between roles that stabilize or destabilize microbial communities depending on phage lifestyle dynamics, host inflammatory state, and ecological context. This Forum discusses these complex roles of phages and proposes a framework to navigate phage functions and unlock virome-targeted therapeutics.
    DOI:  https://doi.org/10.1016/j.chom.2026.04.012
  3. Appl Biosaf. 2026 Mar;31(1): 4-23
       Introduction: Bacteriophages (or phages) are viruses that infect and replicate within bacteria. Widespread use of antibiotics during World War II curtailed phage research in the western world. Unfortunately, clinical deployment of antibiotics has led to the prevalence of antibiotic resistance becoming so severe that the World Health Organization (WHO) has warned about the prospect of a postantibiotic world. The WHO is encouraging research into clinical use of bacteriophages as a means of countering antibiotic resistance.
    Methods: This review summarizes advances in phage research, particularly as it pertains to clinical use. Topics covered include considerations for research and development, genetic engineering, biosafety, One Health (clinical, veterinary, and agricultural use), as well as environmental and regulatory issues.
    Conclusions: Research and development of phage therapy is a promising avenue for combating the dangerous rise in antibiotic resistance and may eventually become a standard medical countermeasure to complement or replace antibiotics.
    Keywords:  FDA guidance; clinical trials; gene editing; gene therapy; long-term follow-up (LTFU); replication competent retrovirus (RCR)
    DOI:  https://doi.org/10.1177/15356760251388486
  4. Wiad Lek. 2026 ;79(4): 829-846
       OBJECTIVE: Aim: This review attempts to examine the microbiology, pathogenesis and current therapeutic approaches of dental caries and periodontal diseases with a special focus on the role of polymicrobial biofilms, the host-microbe interaction and the major pathogenic species involved in disease progression.
    PATIENTS AND METHODS: Materials and Methods: A thorough literature review was performed using major scientific databases such as PubMed, Scopus, Web of Science and Google Scholar. Studies that were published between 2000 and 2025 were included. Relevant experimental, clinical and review articles that focused on the etiology, microbial composition, virulence mechanisms, host immune responses and therapeutic approaches of dental caries and periodontal disease were analyzed.
    CONCLUSION: Conclusions: The oral cavity harbors over 700-800 bacterial species, of which the primary cariogenic pathogen is Streptococcus mutans and Porphyromonas gingivalis has been implicated as a major cause of periodontal disease. Dental caries progression is mostly attributed to acid production and demineralization of enamel, whereas periodontal disease is a result of dysbiotic shift in the subgingival microbiome with destructive host inflammatory responses. The "red complex" (P. gingivalis, Treponema denticola and Tannerella forsythia) has a high degree of synergistic virulence in advanced periodontitis. Biofilm formation, production of extracellular polysaccharide (EPS) matrix, quorum sensing and immune components (neutrophils, macrophages and matrix metalloproteinases or MMPs) are all factors that contribute to disease formation. Prevention strategies include oral hygiene measures, fluoride exposure, dietary modification, and antimicrobial agents, whereas treatment measures include mechanical debridement, systemic antibiotics, antimicrobial peptides, probiotics, and photodynamic therapy. Dental caries and periodontal diseases are the result of complex interactions between polymicrobial biofilms and immune responses by the host. A better understanding of the microbial ecology, virulence pathways and host-pathogen interactions is crucial in the process of improving prevention and treatment. Advances in targeted antimicrobial therapies and innovative therapeutic approaches hold promise for enhancing global oral health outcomes.
    Keywords:   Streptococcus mutant ; biofilm ; dental carriers ; periodontal disease ; quorum sensing
    DOI:  https://doi.org/10.36740/WLek/216932
  5. Oral Dis. 2026 May 12.
       INTRODUCTION: The oral phageome, comprising bacteriophages inhabiting the oral cavity, has been proposed as a potential modulator of periodontal health and disease. This systematic review synthesizes current evidence on interactions between bacteriophages, key periodontal bacteria, and the host in periodontitis.
    METHODS: The review followed PRISMA guidelines and was registered in PROSPERO (CRD420250653631). A systematic search of the literature was conducted from January 2025 up to March 2025. Two independent PECOS strategies were applied to include clinical/observational and ex vivo studies. Risk of bias was assessed using the Newcastle-Ottawa Scale, Joanna Briggs Institute Checklist, and a modified SYRCLE's tool.
    RESULTS: Of 965 records identified, 17 studies, comprising 623 patients, met the inclusion criteria. Clinical studies suggest alterations in the oral phageome in periodontitis, with increased abundance and activity of lytic phages, whereas temperate phages appear more prevalent in periodontal health. Ex vivo studies demonstrate that bacteriophages can interact with major periodontopathogens, influencing bacterial virulence, biofilm behavior, and horizontal gene transfer.
    CONCLUSION: Current evidence does not establish a causal role for bacteriophages in periodontitis. Nevertheless, phages may function as contextual modulators of periodontal dysbiosis, particularly in A. actinomycetemcomitans-associated disease. Further longitudinal and functional multi-omics studies are needed to clarify their clinical relevance.
    TRAIL REGISTRATION: PROSPERO ID: CRD420250653631.
    Keywords:  bacteriophages; dysbiosis; periodontitis; phageome; systematic review
    DOI:  https://doi.org/10.1111/odi.70327
  6. Expert Rev Anti Infect Ther. 2026 May 16. 1-13
       INTRODUCTION: Nontuberculous mycobacteria (NTM) are increasingly recognized as clinically significant pathogens, particularly in immunocompromised individuals and patients with chronic lung disease. A key determinant of their persistence and pathogenicity is the ability to form biofilms. It is important to distinguish biofilm-associated phenotypic tolerance (persistence) from genetic antimicrobial resistance. Biofilm-related persistence is transient and non-genetic, while genetic resistance involves stable heritable changes. Both mechanisms contribute to treatment failure and prolonged infection.
    AREAS COVERED: This review addresses the clinical and therapeutic challenges posed by biofilm-associated NTM infections. We discuss conventional multidrug regimens, the limitations imposed by biofilm physiology, and novel interventions under investigation, including enzymatic biofilm disruption, nanoparticle-based drug delivery, and bacteriophage therapy. Understanding these therapeutic paradigms in the context of biofilm biology is essential for improving outcomes in these difficult-to-treat infections.Given the growing clinical relevance of these organisms, this review primarily examines therapeutic strategies for biofilm-associated NTM infections, while also considering epidemiological and diagnostic aspects to contextualize treatment challenges.
    EXPERT OPINION: Treatment of biofilm-related NTM infections is extremely difficult to cure, and they usually need the implication of a multidisciplinary team for the best management of these infections, while the cure of the patients is, in many cases, impossible.
    Keywords:  Nontuberculous mycobacteria; antimicrobial resistance; biofilms; nanoparticle drug delivery; phage therapy; therapeutic strategies
    DOI:  https://doi.org/10.1080/14787210.2026.2673210
  7. J Appl Microbiol. 2026 May 12. pii: lxag113. [Epub ahead of print]
       AIMS: Multidrug-resistant (MDR) Klebsiella pneumoniae (K. pneumoniae) poses a serious threat to cattle health. This study aimed to isolate phages against bovine-origin MDR K. pneumoniae and evaluate their therapeutic potential.
    METHODS AND RESULTS: We successfully isolated five lytic bacteriophages (phage) (vB_Kpn_Y1-Y5) from hospital sewage samples. They showed favorable temperature and pH stability, efficient lytic cycles, and narrow host ranges. Whole-genome analysis further classified these phages into three categories belonging to the families Siphoviridae and Podoviridae within the class of Caudoviricetes. This genetic diversity provides a foundation for constructing a phage cocktail. A phage cocktail constructed from these phages exhibited synergistic antibacterial effects in vitro, with superior inhibition and duration compared to single phages. In a mouse bacteremia model, the phage cocktail therapy significantly increased the survival rate of mice from 50% to 91.7% (p < 0.05), effectively reduced bacterial loads in multiple organs (p < 0.0001), alleviated tissue inflammatory damage, and showed no obvious toxicity.
    DISCUSSION: The genetic diversity of the phages underpinned their synergy. The phage cocktail demonstrated better efficacy than monotherapy both in vitro and in vivo.
    Keywords:  klebsiella pneumoniae; mouse; multidrug resistance; phage cocktail; phage therapy
    DOI:  https://doi.org/10.1093/jambio/lxag113
  8. Front Cell Infect Microbiol. 2026 ;16 1819965
      Klebsiella pneumoniae (K. pneumoniae) is a critical ESKAPE pathogen with rapidly escalating resistance to last-line antibiotics, for which phages and their derived endolysins have emerged as promising alternatives. Phages specifically recognize bacterial surface structures, including capsular polysaccharide (CPS) and lipopolysaccharide (LPS), with their depolymerases degrading these barriers and disrupting biofilms to attenuate virulence. In parallel, endolysins directly cleave the peptidoglycan layer to induce rapid bacterial lysis. Animal model studies confirm that both phages and endolysins effectively reduce K. pneumoniae burden and improve host survival, with efficacy markedly enhanced when combined with antibiotics or antimicrobial peptides (AMPs). Although narrow host range and potential resistance evolution remain challenges, strategies such as phage cocktails, "phage training", and combination therapies (e.g., phage-antibiotic, phage-AMP, and endolysin-antibiotic combinations) have shown broad application prospects. This review details the antibacterial actions of phages and endolysins against K. pneumoniae, including their mechanisms, anti-biofilm activity, and therapeutic potential in combination therapies.
    Keywords:  Klebsiella pneumoniae; combination therapy; depolymerase; endolysin; phage therapy; phage training
    DOI:  https://doi.org/10.3389/fcimb.2026.1819965
  9. Front Immunol. 2026 ;17 1806445
      Chronic oral inflammatory diseases, particularly periodontitis, are increasingly recognized as important contributors to the onset and progression of systemic disorders. Accumulating epidemiological, clinical, and mechanistic evidence indicates that the oral cavity is not an isolated organ, but rather a critical hub and early window for systemic disease development. Through microbial translocation, chronic low-grade inflammation, immune dysregulation, oxidative stress, and epigenetic reprogramming, oral diseases engage in bidirectional communication with distant organs.We conceptualize this integrated network as the "oral-X axis, " encompassing the oral-cardiovascular, oral-metabolic, oral-respiratory, oral-gastrointestinal, oral-oncologic, oral-immune, oral-brain, and other systemic axes. At the core of these interactions lies periodontitis-associated microbial dysbiosis dominated by key pathogens such as Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. The ensuing inflammatory response compromises periodontal barrier integrity, facilitating the dissemination of bacteria, virulence factors, and inflammatory mediators into the systemic circulation. These processes promote endothelial dysfunction, insulin resistance, breakdown of immune tolerance, neuroinflammation, and the formation of pro-tumorigenic microenvironments, thereby mechanistically linking oral inflammation to a broad spectrum of systemic diseases. This review systematically summarizes the current evidence supporting the oral-X axis, with a particular focus on epidemiological associations and underlying molecular and cellular mechanisms. In addition, we discuss periodontal interventions and oral microbiome modulation as potential strategies for the prevention and treatment of systemic diseases. A deeper understanding of the oral-X axis may provide novel insights into integrated oral-systemic healthcare and precision medicine.
    Keywords:  immune dysregulation; inflammatory overflow; oral - X axis; oral pathogens; oxidative stress
    DOI:  https://doi.org/10.3389/fimmu.2026.1806445
  10. Phage (New Rochelle). 2026 Mar;7(1): 4-8
      Despite the difficult and turbulent history of Russia and the USSR during the period from the 1917 revolution until the end of World War II, in 1945, research on bacteriophage therapy (PT) and basic bacteriophage biology developed in the country starting in the 1920s, concurrently with the development of this field in the West. While practical achievements in PT made in the USSR are quite well known to the scientific community, the efforts of Soviet scientists to discover the nature of phages and PT mechanisms in the 1930s have essentially fallen out of scholarly discourse. Here, I introduce a valuable source of historical information and a beautiful piece of early bacteriophage and PT conceptualization: the monograph by Prof. M.I. Lurie "Twort-d'Hérelle Phenomenon," which appeared days before the beginning of the devastating Great Patriotic War (1941) and was totally obscured by the war events, escaping the attention of historians of science.
    Keywords:  Bacteriophage research in USSR; History of bacteriophage research; Lurie; phage therapy
    DOI:  https://doi.org/10.1177/26416549251408686
  11. J Microbiol Methods. 2026 May 10. pii: S0167-7012(26)00156-9. [Epub ahead of print]246 107544
      Acinetobacter baumannii has emerged as an agent of potentially life-threatening nosocomial infections, particularly among immunocompromised patients. Its ability to rapidly acquire resistance genes has made traditional antibiotic therapies progressively ineffective. The spread of bacterial contamination in hospital facilities increased due to lack of awareness among healthcare workers and improper management/handling of infectious samples. The persistence of pathogen in the hospital environment is increased with its ability to form biofilms, quorum sensing, and virulence factors. The infections caused by these agents are increasing in incidence and severity; necessitating efficient and timely management. This review highlights the epidemiological trends and molecular mechanisms involved in the pathogenesis and resistance of A. baumannii. The key resistance mechanisms that the organism possess include the activity of efflux pumps, beta-lactamase enzymes, and mobile genetic elements. This review discusses emerging treatment strategies - such as phage therapy - antimicrobial peptides, CRISPR-Cas-based technologies, and nanotechnology-enabled drug delivery- highlighting their respective benefits and limitations, with special emphasis on innovations like phage-antibiotic synergy and precision genome editing approaches. Despite promising advances, challenges remain, including the emergence of resistance pathogen, limited clinical scalability, and concern regarding the safety and toxicity of novel treatment options. Addressing these issues require focus on molecular insights of resistance mechanisms, the development of effective alternative therapies, and implementation of preventive strategies such as vaccines. Furthermore, execution of global antimicrobial stewardship program and robust surveillance systems are critical for effectively control and manage the threat posed by A. baumannii.
    Keywords:  AMR; Acinetobacter baumannii; CRAB; Multidrug resistance; Pathogenesis; Phage therapy
    DOI:  https://doi.org/10.1016/j.mimet.2026.107544
  12. PLoS One. 2026 ;21(5): e0349089
      Pseudomonas aeruginosa is a Gram-negative opportunistic bacterium responsible for severe infections such as pneumonia, septicemia, and keratitis. It poses a significant treatment challenge due to its extensive antibiotic resistance and its capacity to form biofilms, which provide bacterial communities with a protective barrier against antibiotics. An increasing number of studies have identified phage therapy as a potential therapeutic solution amidst the current crisis of antibiotic resistance in medicine. Here, we isolated three novel phages in the campus environment and selected one, PW01, for detailed analysis. Host range was determined against clinical isolates, and biological features were evaluated through growth kinetics and biofilm inhibition assays. Whole genome sequencing and annotation were conducted to confirm its lytic nature. In vivo efficacy was assessed using a murine wound infection model. PW01 displayed a relatively broad host spectrum and effectively suppressed bacterial growth in vitro. It disrupted established biofilms and showed genomic features consistent with a strictly lytic lifestyle. In mice, treatment with PW01 combined with antibiotics resulted in greater bacterial reduction compared with either treatment alone. These findings demonstrate that PW01 possesses both in vitro and in vivo activity against P. aeruginosa and support the potential of phage-antibiotic combination therapy as an effective strategy against multidrug-resistant (MDR) infections.
    DOI:  https://doi.org/10.1371/journal.pone.0349089
  13. Front Cell Infect Microbiol. 2026 ;16 1817579
      Pancreatic cancer (PC), particularly pancreatic ductal adenocarcinoma (PDAC), is a highly lethal malignant tumor with poor prognosis, limited early screening strategies, and high chemoresistance. Periodontal disease (PD), a prevalent chronic inflammatory disorder caused by oral microbiota dysbiosis, has been increasingly linked to systemic diseases, including cancer. This review summarizes the epidemiological evidence, potential pathogenic mechanisms, and clinical management implications of the association between PD and PC. Epidemiological studies, including meta-analyses, cohort studies, and case-control studies, consistently demonstrate that PD (especially moderate-to-severe periodontitis and combined gingivitis-periodontitis) is associated with an increased risk of PC incidence and mortality, with significant population heterogeneity (more prominent in middle-aged and elderly individuals, males, and those with comorbid diabetes). Specific periodontal pathogens, such as Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, play key roles in this association, showing dose-response relationships and preceding PC onset. Mechanistically, PD contributes to PC development through multiple interconnected pathways: translocation and colonization of periodontal pathogens in pancreatic tissue, induction of precancerous lesions (e.g., acinar-to-ductal metaplasia), activation of inflammatory signaling pathways (TLR4/NF-κB, Wnt/β-catenin), immune dysregulation and tumor immune escape, microbiota imbalance and carcinogenic metabolite accumulation (e.g., acetaldehyde, nitrosamines), and induction of chemoresistance (via cytidine deaminase-mediated gemcitabine inactivation or Notch1 pathway activation). Clinically, this association provides novel perspectives for PC prevention and management: integrating periodontal health assessment into PC risk screening, developing non-invasive diagnostic biomarkers (oral microbiota, miRNAs, saliva metabolites), optimizing treatment strategies (targeted antibiotics, probiotics, phage therapy), and establishing a multidisciplinary collaboration (MDT) model involving dentistry, oncology, gastroenterology, and microbiology. In conclusion, PD is a potentially modifiable risk factor for PC, and oral health management may serve as a cost-effective strategy for PC prevention and improved prognosis. Future prospective studies are needed to confirm the causal relationship and standardize clinical practices for this cross-organ association.
    Keywords:  chemoresistance; clinical implications; oral microbiota; pancreatic cancer; pathogenic mechanisms; periodontal disease; porphyromonas gingivalis
    DOI:  https://doi.org/10.3389/fcimb.2026.1817579
  14. Curr Vasc Pharmacol. 2026 May 07.
      Various studies have suggested a bidirectional association between periodontal disease and systemic health conditions. Periodontitis is caused by polymicrobial infection, and it affects 7 - 11% of adults worldwide in an age-dependent manner. Besides its local manifestations, including gingival inflammation, periodontal pocket formation, and alveolar bone loss, periodontitis is linked to several systemic conditions. Emerging evidence points to associations with obesity, diabetes, Cardiovascular (CV) Disease (CVD), chronic renal disease, inflammatory bowel disease, respiratory diseases, rheumatoid arthritis, unfavorable pregnancy outcomes, specific cancers, neurodegenerative disorders, psychological diseases, and autoimmune disorders. These links seem to be mediated by 3 main mechanisms: dysbiotic oral biofilms, chronic low-grade systemic inflammation, and the spreading of periodontal pathogenic microbes throughout the body. The pathophysiologic mechanisms involved comprise high concentrations of pro-inflammatory cytokines (e.g., tumor necrosis factor alpha, interleukin 6), increased C-reactive protein, worsened immune function, oxidative stress, and molecular mimicry. Certain periodontal pathogenic microorganisms (e.g., Porphyromonas gingivalis) play a role in triggering and maintaining systemic inflammatory responses. Importantly, management of periodontitis has led to improvements in various systemic conditions, underscoring the clinical importance of these links. Thus, periodontitis represents more than a local oral disease; it significantly contributes to the overall systemic inflammatory burden, with implications for general and cardiovascular health. Therefore, an organized multidisciplinary approach for the prevention, early detection, and optimal management of periodontal disease is essential for improving both cardiovascular and overall patient outcomes. In this respect, healthcare providers should regard oral health as a crucial and integral component of systemic health.
    Keywords:  Periodontal disease; cardiovascular disease; cardiovascular health; chronic kidney disease; diabetes; gingivitis.; hypertension; inflammation; obesity; oxidative stress; periodontitis; smoking
    DOI:  https://doi.org/10.2174/0115701611433483260423072408
  15. Front Microbiol. 2026 ;17 1823476
      Biofilm mediated antimicrobial resistance (AMR) has become a critical global health and economic challenge, affecting both community and healthcare settings. Microbial Biofilms significantly enhance the antibiotic tolerance and cause the persistent and device-associated infections via limited drug penetration, degradation of antibiotics, and assist horizontal gene transfer. Biofilm-mediated antimicrobial resistance remains a major obstacle to treating infectious diseases today. Biofilms can boost antibiotic tolerance by up to 1,000 times and lead to chronic, persistent, and device-associated infections. The lack of FDA-approved anti-biofilm drugs highlights the urgent need for new therapeutic strategies and mechanistic insights. Redefining the treatment landscape and improving outcomes for resistant infections could be achieved through a multi-platform therapeutic approach. This review summarizes recent developments in our knowledge of how biofilms contribute to antibiotic resistance and highlights new therapeutic strategies, such as nanotechnology, antimicrobial peptides, bacteriophage-derived enzymes, quorum-sensing inhibitors, CRISPR-based tools, microbiome engineering, and AI-driven drug discovery.
    Keywords:  ESKAPE pathogens; antimicrobial resistance; biofilm-associated antibiotic resistance; combination therapy; extracellular matrix
    DOI:  https://doi.org/10.3389/fmicb.2026.1823476
  16. Infection. 2026 May 11.
      Multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa) has emerged as a major threat in contemporary clinical microbiology, driven by the convergence of extensive antimicrobial resistance, biofilm-mediated persistence, and tightly coordinated virulence regulation. Global surveillance reveals a sustained increase in MDR and carbapenem-resistant P. aeruginosa (CRPA), characterized by marked regional heterogeneity and the dissemination of high-risk international clones. Conventional antibiotic-centered strategies are increasingly compromised by the interplay between intrinsic resistance determinants, including low outer membrane permeability, multidrug efflux systems, and inducible β-lactamase, and acquired mechanisms such as carbapenemases, target-site mutations, and adaptive biofilm-associated tolerance. This review presents a systems-level framework that integrates global epidemiology, resistance architecture, clinical manifestations of acute and chronic infections, pathogenicity, diagnostic innovation, and emerging therapeutic strategies into a cohesive translational perspective. Emphasizing the mechanistic convergence of resistance, persistence, and virulence reframes MDR P. aeruginosa as a dynamically regulated pathogenic network rather than solely a drug-resistant organism. Next-generation interventions are critically examined within the context of precision diagnostics and antimicrobial stewardship. Taken together, these developments signal a shift from antibiotic escalation toward integrated, precision-informed, and evolution-aware therapeutic frameworks. Sustainable control of MDR P. aeruginosa will require coordinated implementation of targeted pathogen disruption, rapid molecular diagnostics, robust stewardship strategies, hygiene measures, and antimicrobial resistance surveillance to redefine antimicrobial practice as we get closer to the post-antibiotic era.
    Keywords:  Antibiotics; Antimicrobial resistance mechanisms; Biofilm; Carbapenem resistance; Multidrug-resistant Pseudomonas aeruginosa ; Precision therapeutics; Prevalence; Virulence regulation
    DOI:  https://doi.org/10.1007/s15010-026-02817-8
  17. J Clin Nurs. 2026 May 11.
       AIM: To examine and map the available literature on outcomes associated with interventions carried out by advanced practice nurses in chronic wound care.
    BACKGROUND: The role of the advanced practice nurses in wound care is identified as a key element in the management of patients with chronic wounds. However, the literature offers fragmented knowledge of the outcomes associated with their practice.
    DESIGN: Scoping review.
    METHODS: This scoping review was conducted following the methodological framework proposed by Arskey and O'Malley, following the Preferred Reporting Item for Systematic Review and Meta-analysis for Scoping Review. PRISMA-ScR Checklist is included in the manuscript. Observational or experimental studies related to patients affected by chronic wounds and cared for by advanced practice nurses in wound care were included.
    DATA SOURCES: The following databases were queried: PubMed, CINAHL, Cochrane Library, and Scopus from 01 May 2025 to 31 October 2025.
    RESULTS: The search strategy in the consulted databases identified 1956 studies; 31 met the inclusion criteria. Different types of chronic wounds were investigated: diabetic foot ulcers, venous leg ulcers, and pressure injuries. The most frequently measured outcomes were clinical responses (healing, recurrence, complications), organizational efficiency (referrals, resource utilization), and patient-reported outcomes.
    CONCLUSION: Evidence from the included studies suggests that Advanced practice nurses led wound care models may be associated with faster healing, lower recurrence and complication rates, and more efficient care pathways, across different settings. This review highlights the global applicability of APN-led models, showing consistent improvements in clinical, organizational, and patient-reported outcomes through core interventions.
    REPORTING METHOD: This scoping review follows Arskey and O'Malley's methodological framework and the Preferred Reporting Item for Systematic Review and Meta-analysis for Scoping Review.
    PATIENT OR PUBLIC CONTRIBUTION: No Patient or Public Contribution.
    PROTOCOL REGISTRATION: The revision protocol was registered on the OSF (https://doi.org/10.17605/OSF.IO/P9TA4).
    Keywords:  advanced practice nurses; chronic wounds; diabetic foot ulcers; health outcomes; literature review; pressure injuries; venous leg ulcers; wound care
    DOI:  https://doi.org/10.1111/jocn.70355
  18. Front Cell Infect Microbiol. 2026 ;16 1826282
      Biofilms are highly organised complex structures formed by microorganisms that adhere to surfaces and are embedded with an extracellular polymeric matrix. This matrix provides structural stability, retains nutrients and offers defence against unfavourable environments and antibiotics. Multi-layered molecular mechanisms controlled by quorum-sensing networks are involved in the transition from the planktonic stage to a mature biofilm. Surface attachment, maturation and dispersion are coordinated by these mechanisms, which also provide communication between different species. Biofilm development poses a significant challenge to implants in nosocomial settings and is considered a major threat in the global health care sector and industries, leading to persistent infection. In addition to assessing current biofilm management techniques such as quorum quenching agents, enzymatic matrix disruptions, antimicrobial peptides, nanoparticles and metal complex-based interventions, this review highlights the major regulatory components and molecular mechanisms causing biofilm formation. It also emphasises the necessity to combat biofilm-associated tolerance by highlighting the increasing significance of computational approaches in drug discovery and development of next-generation anti-biofilm therapeutics.
    Keywords:  antimicrobial resistance; biofilm; extracellular matrix; peptides; quorum sensing
    DOI:  https://doi.org/10.3389/fcimb.2026.1826282
  19. Int J Antimicrob Agents. 2026 May 11. pii: S0924-8579(26)00127-5. [Epub ahead of print] 107838
       BACKGROUND: Antimicrobial resistance is a global health problem, with methicillin-resistant Staphylococcus aureus (MRSA) causing significant morbidity and mortality. As antibiotic options dwindle, (bacterio)phage therapy is re-emerging as a promising alternative.
    METHODS: We characterized a novel lytic S. aureus phage, PBSA08, and evaluated its host range using a reference panel of 39 MRSA isolates selected from the 2021 Australian Group on Antimicrobial Resistance repository. Phage susceptibility was tested by efficiency of plating (EOP) and growth kinetics assays (GKA). Factors associated with phage susceptibility were evaluated.
    RESULTS: Phage PBSA08, classified under the genus Silviavirus, revealed an icosahedral head and long tail structure using transmission electron microscopy. Adsorption assays indicated efficient bacterial binding, with a burst size of approximately 120 plaque forming units (PFU) per infected cell. EOP testing revealed 51.3% of the MRSA reference panel isolates were susceptible (EOP ≥10%). GKA results correlated strongly with EOP. In univariate analysis, phage PBSA08 susceptibility was associated with multilocus sequence type (ST), ciprofloxacin susceptibility, and the presence of the Panton-Valentine leucocidin-associated genes, with all ST30-IV and ST93-IV isolates susceptible to PBSA08 and all ST45-V isolates resistant to PBSA08. Firth logistic regression showed that ST30/93 isolates had significantly higher odds of susceptibility (aOR 68.9, 95% CI 2.0-2378.5; p=0.0191), as did ciprofloxacin-susceptible strains (aOR 15.4, 95% CI 1.7-143.0; p=0.0160).
    CONCLUSION: PBSA08 demonstrates potential as a therapeutic phage, particularly against community-associated MRSA lineages such as ST93. Its inclusion in phage cocktails targeting prevalent MRSA clones warrants further investigation.
    Keywords:  Staphylococcus aureus; Susceptibility; phage therapy
    DOI:  https://doi.org/10.1016/j.ijantimicag.2026.107838
  20. Antonie Van Leeuwenhoek. 2026 May 14. pii: 125. [Epub ahead of print]119(6):
      Adult periodontitis is a chronic inflammatory disease characterized by microbial dysbiosis and host-microbe imbalance in the oral cavity. In this study, the composition and antibiotic susceptibility of the salivary microbiota in adults with mild periodontitis were analyzed to emphasize the influence of lifestyle factors on microbial ecology and resistance trends. Saliva samples collected from clinically diagnosed individuals were analyzed using selective culture-based methods and the Kirby-Bauer disc diffusion assay. Statistical analyses were performed to evaluate interspecies growth variability and antimicrobial responses. It revealed distinct salivary microbial profiles in patients with periodontitis compared to those of healthy individuals. Fusobacterium nucleatum exhibited the highest optical density, indicating increased proliferation and potential involvement in disease progression. Lifestyle variables, particularly smoking and dietary habits, significantly influenced microbial composition (r = 0.73 for smoking; r = 0.59 for tobacco use). Antibiotic susceptibility testing has revealed substantial interspecies variation, with ciprofloxacin and tetracycline showing the highest inhibitory efficacy, whereas azithromycin and clindamycin were largely ineffective. Moderate positive correlations between Prevotella, Clostridium, and F. nucleatum (r = 0.61-0.65) suggest possible shared resistance mechanisms or ecological adaptation. Overall, combined effects of behavioral and microbial factors can shape early periodontal dysbiosis and antibiotic resistance.
    Keywords:  Antibiotic resistance; Life Style; Microbiota; Periodontitis; Precision medicine; Saliva
    DOI:  https://doi.org/10.1007/s10482-026-02336-4
  21. Mol Biol Rep. 2026 May 13. pii: 754. [Epub ahead of print]53(1):
      Diabetic foot ulcers are a major cause of morbidity in patients with diabetes mellitus and remain challenging to treat despite advances in wound care. Growing evidence suggests that impaired axonal regeneration, along with chronic inflammation, impaired angiogenesis, and altered extracellular remodeling, is an important mechanism contributing to the chronic, nonhealing nature of cutaneous wounds. However, the mediators involved and the mechanisms by which they impair wound healing in the presence of hyperglycemia are poorly understood. Peripheral nerves coordinate inflammation, angiogenesis, and extracellular matrix remodeling during repair, and their dysfunction in diabetes disrupts these processes. This review examines how hyperglycemia and oxidative stress impair neuroregeneration ( mainly axonal and nerve regeneration), highlighting the downstream consequences for vascular and structural repair. Particular attention is given to the altered expression of activin A (associated with nerve growth and neural inflammation), TNFRSF10B (associated with neuronal damage and apoptosis, and synaptophysin (a marker of synaptic vesicles and involved in nerve regeneration, particularly in the early stages of axonal regrowth) as representative mediators linking neuronal injury to defective wound healing. By integrating findings across neural, vascular, and inflammatory pathways, this review supports impaired neuronal regeneration as an important contributor to nonhealing diabetic foot ulcer pathogenesis to identify potential molecular targets that may improve healing outcomes.
    Keywords:  Activin A; Axonal regeneration; Nonhealing diabetic foot ulcers; Synaptophysin; TNFRSF10B; Wound healing
    DOI:  https://doi.org/10.1007/s11033-026-11941-5
  22. Adv Wound Care (New Rochelle). 2026 Jul;15(7): 469-480
    NIDDK Diabetic Foot Consortium
       OBJECTIVE: Platform studies are an innovative clinical trial design to evaluate multiple potential therapies simultaneously using a master protocol framework for more rapid discovery of critical new disease-modifying therapies. Initially pioneered in cancer clinical trials, platform studies have more recently been adopted for new vaccine trials and other disease states. This approach has not been used for biomarker(s) validation.
    APPROACH: We present the development and successful implementation of a master protocol for a platform study in the National Institute of Diabetes and Digestive and Kidney Diseases Diabetic Foot Consortium (DFC) that concomitantly tests multiple biomarkers that could be used to predict diabetic foot ulcer (DFU) healing outcomes in people with DFUs.
    RESULTS: This flexible master protocol, allowing the study of multiple biomarkers in a perpetual manner, designed to be inclusive of a broad spectrum of people with DFUs operating under the mantra "No DFU Goes Unstudied" successfully collects participant data and biospecimens for biomarker development and validation in a standardized manner across multiple DFC sites.
    INNOVATION: Integrating multiple biomarkers with a rich database of participants' clinical characteristics, patient-reported outcomes, and standard of care practices at various sites collected longitudinally over 52 weeks following a pragmatic approach and thus relevant to people with DFU as seen in today's daily practice, enable their subsequent implementation at the point of care to guide timely personalized DFU management decisions.
    CONCLUSION: Leveraging this robust platform will help to identify new therapeutic targets and design and conduct effective interventional clinical trials for DFU wound healing, which could inform the standards of care.
    Keywords:  biomarkers; clinical network; diabetic foot ulcers; wound healing
    DOI:  https://doi.org/10.1177/21621918251405881
  23. Front Cell Infect Microbiol. 2026 ;16 1809787
       Background: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis. Although the oral microbiome has been characterized in diverse populations, comprehensive profiling across periodontal disease stages defined by the 2018 AAP/EFP classification remains limited in Korean adults.
    Methods: In this pilot prospective cross-sectional study, oral microbiome profiles were characterized in 74 participants classified into three groups: healthy controls (n = 24), Stage I-II periodontitis (n = 12), and Stage III-IV periodontitis (n = 38). Mouthwash samples were collected and subjected to 16S rRNA gene sequencing of the V3-V4 hypervariable region. Alpha diversity, beta diversity (PERMANOVA with sequential covariate adjustment for age, sex, and smoking), differential abundance (MaAsLin2), and core microbiome analyses were performed.
    Results: Stage III-IV periodontitis was associated with significantly higher Shannon diversity, Simpson diversity, and Pielou's evenness compared to both healthy and Stage I-II groups, indicating increased evenness rather than species richness. Beta diversity analyses revealed significant community-level separation across groups after adjustment for demographic confounders (allp = 0.001). Differential abundance analysis identified 14 genera significantly associated with disease status. Twelve genera were enriched in Stage III-IV, including established periodontal pathogens Tannerella and Treponema, as well as emerging pathobionts Filifactor and Fretibacterium. Rothia and Kingella were enriched in periodontal health, consistent with their roles in nitrate reduction and maintenance of a health-compatible oral environment. Core microbiome analysis identified 40 universally present genera, with Anaeroglobus detected exclusively in Stage III-IV at 100% prevalence.
    Conclusion: These findings support the polymicrobial synergy and dysbiosis model of periodontitis pathogenesis and provide a foundation for developing microbiome-based diagnostic tools for periodontal disease assessment in Korean populations.
    Keywords:  16S rRNA sequencing; Korean population; microbial diversity; mouthwash; oral microbiome; periodontitis
    DOI:  https://doi.org/10.3389/fcimb.2026.1809787
  24. Ann Plast Surg. 2026 May 11.
      Effective wound healing depends on timely optimization of the wound environment and appropriate reconstructive strategy. Impaired healing, often driven by infection, ischemia, or systemic factors, increases the risk of chronic wounds and adverse scarring. This review highlights key principles of wound bed preparation, including assessment, debridement, infection control, and appropriate dressing selection. For wounds that fail to progress with standard care, advanced interventions such as skin grafting and cellular and tissue-based products play a critical role in promoting closure and improving outcomes. A structured, algorithmic approach to wound management is essential to optimize healing and minimize complications.
    Keywords:  debridement; healing; ulcers; wound
    DOI:  https://doi.org/10.1097/SAP.0000000000004769
  25. Nature. 2026 May 13.
      
    Keywords:  Antibiotics; Machine learning; Microbiology
    DOI:  https://doi.org/10.1038/d41586-026-01424-9
  26. Cureus. 2026 May;18(5): e108721
       INTRODUCTION: The aim of our study was to learn about the effect of bacteriophages derived from the water of the river Ganga on Pseudomonas-induced corneal ulcer in mice. Pseudomonas aeruginosa poses a significant threat in cases of bacterial keratitis, a condition that severely hampers vision. With variable incidence rates worldwide and increasing antibiotic resistance to Pseudomonas, it is necessary to explore the potential of bacteriophages isolated from Ganga water for treating Pseudomonas aeruginosa-induced keratitis.
    MATERIAL AND METHODS: We used a mouse model for this study. The most potent phages targeting Pseudomonas aeruginosa that demonstrated a broad spectrum of activity against clinical strains were phenotypically and genotypically characterised. Establishing the animal model involved inducing severe keratitis in mice by inoculating them with Pseudomonas aeruginosa. After this, we administered multiple doses of bacteriophages as eye drops, 24 hours after infection.
    RESULTS: The results yielded significantly improved disease outcomes. The treatment not only preserved the structural integrity and transparency of the infected cornea but also suppressed neutrophil infiltration and bolstered bacterial clearance, as evidenced by histopathological analysis.
    CONCLUSION: These findings suggest that bacteriophage therapy can be an effective alternative therapeutic approach for treating antibiotic-resistant infectious keratitis. Also, we can use bacteriophages in combination with antibiotics already in use.
    Keywords:  bacteriophage therapy; corneal ulceration; pseudomonas aeruginosa (p. aeruginosa); rat model study; therapeutic intervention efficacy
    DOI:  https://doi.org/10.7759/cureus.108721
  27. Cell Host Microbe. 2026 May 13. pii: S1931-3128(26)00170-8. [Epub ahead of print]34(5): 811-813
      Locked in a constant arms race, bacteria and their phage predators have evolved various defenses and counter-defenses. Compared to the numerous identified defenses, phage-encoded counter-defenses are understudied. In a recent Science paper, Tal et al. developed a structure-guided approach to identify phage proteins counteracting nucleotide signaling defenses using metagenomic data.
    DOI:  https://doi.org/10.1016/j.chom.2026.04.014
  28. ERJ Open Res. 2026 Mar;pii: 00740-2025. [Epub ahead of print]12(2):
      Bronchiectasis is a chronic respiratory disease characterised by permanent bronchial dilation, productive cough and recurrent exacerbations. Staphylococcus aureus (including methicillin-resistant strains (methicillin-resistant S. aureus (MRSA)) is increasingly recognised as a relevant pathogen in bronchiectasis, although its role remains less well defined than that of Pseudomonas aeruginosa or Haemophilus influenzae. This narrative review examines the epidemiological, pathophysiological, clinical and therapeutic implications of S. aureus infection in adults with bronchiectasis, drawing comparisons with other disease models. Epidemiological data reveal a lower prevalence of S. aureus in bronchiectasis compared to other pathogens, with significant regional variation. Its clinical impact is debated, with some studies associating chronic S. aureus infection with more severe disease, while others consider it a marker of severity rather than a direct driver of progression. Unlike P. aeruginosa, current guidelines do not recommend specific eradication or long-term suppressive strategies for S. aureus. British guidelines suggest MRSA eradication may be considered in cases of clinical deterioration, although no standardised protocol exists. Exacerbations are managed with a 14-day course of targeted antibiotics. Preventive strategies, including decolonisation and vaccine development, are under investigation or adapted from other clinical contexts. While no vaccine is currently available, monoclonal antibodies targeting S. aureus toxins have shown promise in early trials. Further research is needed to clarify the pathogen's role in disease progression and the efficacy of targeted therapies. This review also outlines clinical practice points and research priorities to support evidence-based management.
    DOI:  https://doi.org/10.1183/23120541.00740-2025
  29. J Nanobiotechnology. 2026 May 09.
      This study investigated the relationship between periodontitis and metabolic dysfunction-associated steatotic liver disease (MASLD), with a specific focus on the role of Porphyromonas gingivalis peptidylarginine deiminase (PPAD) carried by P. gingivalis-derived outer membrane vesicles (OMVs) in hepatic lipid metabolism. Clinical analyses revealed a positive correlation between periodontal disease severity and the fatty liver index (OR = 6.18, 95% CI: 2.36-16.13), a predictor of MASLD. In an obese mouse model of periodontitis, P. gingivalis-induced periodontitis accelerated MASLD progression, promoted hepatic lipid accumulation and increased inflammation. Since P. gingivalis OMVs had the advantage of traveling through the blood to the liver, we found that they demonstrated liver-specific accumulation, impairing fatty acid oxidation and increasing lipid deposition in hepatocytes, when injected into MASLD model mice via the tail vein. The critical role of PPAD was confirmed using the P. gingivalisΔppad and P. gingivalisppad-oe strains, from which OMVs were isolated. The results of the in vivo and in vitro experiments indicated that PPAD-enriched OMVs mediated hepatic metabolic dysregulation via suppression of the circadian regulator NPAS2 and downstream inhibition of CYP4A10 expression, revealing a previously unrecognized PPAD-liver communication pathway. These findings highlight the urgent need for effective PPAD inhibitors and a deeper understanding of the interactions of PPAD with host proteins implicated in systemic diseases, underscoring the broader health impacts of periodontal disease.
    Keywords:   P. gingivalis OMVs; MASLD; NPAS2; peptidylarginine deiminase; periodontitis
    DOI:  https://doi.org/10.1186/s12951-026-04523-x
  30. Diabetes Res Clin Pract. 2026 May 13. pii: S0168-8227(26)00214-7. [Epub ahead of print]237 113294
      Diabetic foot osteomyelitis (DFO) represents a severe manifestation of diabetic foot syndrome and a leading cause of lower-limb amputation globally. Surgical intervention is frequently required, often leaving residual bone defects that can promote persistent infection and impair healing. In this context, bioabsorbable antibiotic-loaded bone substitutes have emerged as a potential adjunct in surgical management. These biomaterials enable high local antibiotic delivery within the surgical cavity, exceeding minimum inhibitory concentrations for common pathogens, while avoiding systemic toxicity. In parallel, they act as fillers of dead space and provide an osteoconductive scaffold that supports bone regeneration. This narrative review summarizes the pathophysiology of DFO, current surgical principles, and the properties of available antibiotic-loaded bone substitutes. We also critically appraise the clinical evidence supporting their use. Available studies suggest promising outcomes in terms of infection control, wound healing, and limb preservation. However, the current evidence base is largely limited to observational and heterogeneous studies, with variability in study design, patient selection, and biomaterial characteristics. Overall, while these technologies appear to be a valuable adjunct in selected cases, robust prospective and randomized trials are needed to better define their indications, comparative effectiveness, and long-term clinical impact in the management of diabetic foot osteomyelitis.
    Keywords:  Antibiotics; Bioabsorbable antibiotic-loaded bone substitutes; Bone defects; Diabetic foot osteomyelitis
    DOI:  https://doi.org/10.1016/j.diabres.2026.113294
  31. Rev Endocr Metab Disord. 2026 May 13.
      Diabetic foot ulcers are serious skin wounds that affect many people with diabetes, often leading to severe infections or even the loss of a limb. This paper explores how artificial intelligence -computer programs that can learn from data-is changing the way doctors find and treat these wounds. By reviewing 68 recent studies, we looked at how these smart technologies analyze different types of medical images to help patients. Our findings show that AI can help doctors identify health risks much earlier than traditional methods. These computer tools are also excellent at measuring how a wound is healing and predicting which treatments will work best for each individual. Because AI can spot tiny patterns in images that the human eye might miss, it makes medical care more precise and consistent. In conclusion, using AI to manage diabetic foot wounds offers a powerful way to improve patient health. By helping doctors make better, data-driven decisions, this technology can lead to faster healing and reduce the risk of serious complications for people living with diabetes.
    Keywords:  Artificial intelligence; Diabetic foot ulcer; Diagnosis and treatment workflow; Multi-modal imaging; Radiomics
    DOI:  https://doi.org/10.1007/s11154-026-10041-w
  32. J Ginseng Res. 2026 May;50(3): 100990
       Background: Diabetes-related wounds or ulcers are one of the complications of diabetes that can cause a variety of functional losses. Treatment of diabetic skin ulcer (DSU) focuses on alleviating foot pressure and enhancing local circulation. Despite the availability of these treatments, the number of drugs for wound application is limited. In recent years, natural compounds obtained from plants have demonstrated positive effects on DSU and have effective in preclinical and clinical studies.
    Purpose: The objective of the study was to assess the diabetes-related wounds or ulcers of natural compounds and the potential mechanisms.
    Study design: and Methods: The study searched PubMed database up to January 2025.
    Results: In the review, the efficacy and safety of natural compounds in treating diabetes-related wounds or ulcers are summarized from 42 preclinical and 13 clinical studies.
    Conclusion: Overall, these preclinical and clinical studies advocate for the use of natural compounds in diabetes-related wounds or ulcers, indicating that ursolic acid, paeoniflorin, and Curcuma zedoaria polysaccharide (ZWP) could be effective and well-tolerated potential medications.
    Keywords:  Clinical studies; Diabetes-related wounds or ulcers; Natural compounds; Preclinical studies; Traditional medicines
    DOI:  https://doi.org/10.1016/j.jgr.2026.100990
  33. J Am Med Inform Assoc. 2026 May 12. pii: ocag074. [Epub ahead of print]
      Artificial intelligence (A.I.) technologies are increasingly deployed across clinical care, yet reimbursement remains a major barrier to sustainable adoption. The Centers for Medicare & Medicaid Services (CMS) currently reimburses A.I.-enabled technologies through a fragmented set of procedural codes, add-on payments, and legacy payment models, none of which were designed to support the complexity or workflow integration of clinical A.I. This article examines how existing CMS reimbursement pathways for A.I. function in practice, identifies structural misalignments that limit adoption, and highlights the risks of continuing to rely on these approaches. We then propose policy-level solutions to modernize A.I. reimbursement, including clearer coverage pathways, value-aligned payment models, and mechanisms to promote equitable adoption across diverse healthcare settings. Aligning reimbursement with clinical value is essential to ensure that A.I. improves care delivery and can be sustainably integrated into routine clinical practice.
    Keywords:  Artificial intelligence; health policy; healthcare reimbursement; medicare policy; value-based care
    DOI:  https://doi.org/10.1093/jamia/ocag074
  34. Animals (Basel). 2026 Apr 23. pii: 1302. [Epub ahead of print]16(9):
      Salmonella enterica remains a major threat to animal and human health because of its broad host range, increasing antimicrobial resistance (AMR), and capacity to form biofilms. Biofilm formation enhances bacterial persistence in host tissues, farm environments, food-processing systems, and clinical reservoirs, while also contributing to their tolerance against antibiotics, disinfectants, and other stresses. However, biofilm capacity is not uniform across serovars and is influenced by host adaptation, niche specialization, and accessory genome content. This review synthesizes current knowledge on the relationship between biofilm formation, AMR, and serovar-specific adaptation in Salmonella. It examines biofilm-associated traits across various hosts (e.g., gastrointestinal tract and gallbladder, and environmental (e.g., food-production and clinical) niches, and discusses comparative evidence from genomic, transcriptomic, proteomic, and metabolomic studies. Particular attention is given to the emerging concept of comparative biofilmomics, which integrates phenotypic and multi-omics data across diverse serovars and host sources to identify conserved and niche-specific determinants of persistence. This framework may help define high-risk lineages that couple multidrug resistance (MDR) with enhanced biofilm-forming capacity. A better understanding of these linked traits will support the development of more targeted interventions for controlling persistent Salmonella in veterinary, food production, and public health settings.
    Keywords:  Salmonella enterica; antimicrobial resistance; biofilm formation; comparative biofilmomics; food safety; host adaptation; multi-omics integration; multidrug resistance; persistence; serovar-specific adaptation
    DOI:  https://doi.org/10.3390/ani16091302
  35. Microb Drug Resist. 2026 May 13. 10766294261448636
      Newer methicillin-resistant antimicrobials are needed to address the rising prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative (MRCONS) infections. We describe two contrasting, recalcitrant staphylococcal infections successfully managed using early transition to the oral benzoquinolizine antibiotic alalevonadifloxacin, highlighting its utility in complex clinical settings. Two high-severity cases of staphylococcal infections are presented. Patient 1, a 58-year-old metastatic lung and orthopedic implant infection on hemodialysis, developed catheter-associated MRSA sepsis with bacteremia, endocarditis, and metastatic lung and orthopedic implant infection. Patient 2, a 41-year-old male with a prior craniotomy, presented with a chronic cranial wound infection due to methicillin-resistant Staphylococcus hominis (MRCONS) originating from an infected ex vivo bone flap. Both patients initially received intravenous therapy and were transitioned to oral alalevonadifloxacin upon availability of susceptibility data. Levonadifloxacin susceptibility testing showed an MIC of 2 µg/mL for MRSA and 0.047 µg/mL for MRCONS-SCV. Patient 1 received 7 weeks of oral therapy aligned with POET recommendation for endocarditis, while Patient 2 completed 8 weeks of oral therapy consistent with OVIVA-based management of osteomyelitis. Despite significant challenges, both patients demonstrated rapid clinical improvement, resolution of infection, and no relapse during follow-up. These cases support oral alalevonadifloxacin as an effective and safe step-down option for complex MRSA and MRCONS infections, including endocarditis, osteomyelitis and meningitis. Alalevonadifloxacin (prodrug of levonadifloxacin) offers a promising oral treatment option when conventional agents are not suitable.
    Keywords:  MRSA; OVIVA; POET; levonadifloxacin
    DOI:  https://doi.org/10.1177/10766294261448636
  36. Nature. 2026 May;653(8114): S18-S19
      
    Keywords:  Antibiotics; Drug discovery; Public health; Therapeutics
    DOI:  https://doi.org/10.1038/d41586-026-01379-x