bims-fagtap Biomed News
on Phage therapies and applications
Issue of 2026–08–23
34 papers selected by
Luca Bolliger, lxBio



  1. Arch Microbiol. 2026 Aug 18. pii: 580. [Epub ahead of print]208(11):
      Diabetic foot infections (DFIs) are a significant public health problem, associated with a delayed healing process and high rates of recurrence, which culminates in amputation. Two main factors, antimicrobial resistance (AMR) and biofilm formation, are responsible for the persistence and therapeutic failure of DFIs, resulting in extended healing time, infection recurrence, and an increased risk of amputation. In addition, the emergence of multidrug-resistant (MDR) pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa has made traditional antibiotic treatment less effective, necessitating alternative or adjunctive therapy. Phage therapy is an alternative approach to treat biofilm-associated and antimicrobial-resistant DFIs. Bacteriophages, viruses that infect bacteria, are highly specific to their bacterial hosts, can disrupt biofilms, and increase the activity of antimicrobial drugs used alone or in combination. This review focuses on the therapeutic potential of phage-based interventions for AMR and biofilm-related DFIs, highlighting delivery methods, phage-antibiotic synergy (PAS), incorporation into wound care regimens, and novel translational potential. Further interest in phage-based therapeutics has grown with recent advances in engineered phages, phage-derived enzymes, and precision diagnostics. Clinical and preclinical data indicate that phage therapy may be a promising strategy to improve bacterial control in specific DFI applications. Experimental studies have shown activity against MDR pathogens and biofilm-associated infections, and early clinical reports show potential for therapeutic benefit. The evidence base is currently small and is skewed towards in vitro studies, animal models, case reports, and small clinical trials. However, significant clinical evidenceis still needed before they can be widely adopted. There are several important barriers, such as the absence of large-scale randomized controlled trials, standardized treatment protocols, manufacturing consistency, and harmonized regulatory frameworks. Rigorous clinical evaluation, enhanced diagnostics (e.g., metagenomics profiling), delivery optimization, and regulatory coordination will be the key factors for further progress. Together, these advances could facilitate the integration of phage therapy into a multidisciplinary approach to DFI treatment and improve outcomes for patients with complex biofilm-related and AMR infections.
    Keywords:  Antimicrobial resistance; Bacteriophage therapy; Biofilm-associated infections; Diabetic foot infections; Phage-antibiotic synergy
    DOI:  https://doi.org/10.1007/s00203-026-05134-8
  2. Infect Drug Resist. 2026 ;19 619916
      Antimicrobial resistance (AMR) continues to compromise the effectiveness of conventional antibacterial therapy, driving the development of therapeutic strategies that extend beyond direct antibiotic-mediated bacterial killing. Multidrug-resistant (MDR) pathogens evade treatment through diverse mechanisms, including enzymatic drug inactivation, target modification, efflux pump overexpression, biofilm formation, and persisters development. AMR results in chronic and recurrent infections, prolonged hospitalization, increased healthcare costs, and elevated morbidity and mortality, underscoring the need for innovative therapeutic approaches that target both the pathogen and the host. To bridge the dynamic interplay between bacterial pathogens and the host immune system with emerging therapeutic innovations, this narrative review first examines the biological mechanisms underlying bacterial resistance. It then explores therapeutic strategies beyond conventional antibiotics, providing an overview of current approaches and their limitations, including drug repurposing, bacteriophage therapy, and CRISPR-Cas technology. The review subsequently focuses on antibacterial immunotherapy, discussing a broad range of emerging approaches, including probiotics, monoclonal antibodies, cell-based therapies, host-directed therapies, aptamers, nanotechnology-based platforms, cytokine-based therapies, and antimicrobial peptides. An integrated overview of preclinical evidence, clinical studies, and FDA-approved therapies is presented to assess the translational potential of immunotherapy strategies in combating AMR. Scientific, regulatory, manufacturing, and implementation challenges that influence their successful translation into clinical practice are discussed throughout. By integrating the biological basis of host-pathogen interactions with emerging antibacterial therapeutics and their translational development, this review provides a comprehensive framework for evaluating innovative strategies against antimicrobial resistance. In contrast to modality-focused reviews, it offers a unified perspective that highlights the complementary roles of pathogen-targeted and host-directed interventions and identifies future opportunities to improve the prevention and management of multidrug-resistant bacterial infections.
    Keywords:  antibacterial immunotherapy; antimicrobial resistance; aptamers; bacteriophage therapy; immune evasion; nanotechnology
    DOI:  https://doi.org/10.2147/IDR.S619916
  3. Infection. 2026 Aug 17.
       PURPOSE: Antimicrobial resistance (AMR) represents a growing challenge across human, animal, and environmental health sectors. Bacteriophage therapy is a potential strategy against methicillin-resistant Staphylococcus aureus (MRSA), one of the most significant contributors to AMR-associated morbidity and mortality globally. One Health promotes coordinated action across sectors to address AMR. This scoping review directly compares the therapeutic potential of bacteriophage therapy to antibiotic treatment for MRSA infections using bacterial load as the primary outcome measure and examines the implications of these findings within a One Health framework for combatting AMR.
    METHODS: PubMed, CINAHL, and Scopus were searched for articles published from 2010 to 2026. Inclusion criteria were studies comparing bacteriophage therapy with antibiotics regimens for MRSA infections and reporting quantifiable bacterial load outcomes.
    RESULTS: Out of 2,160 articles identified, 29 met the inclusion criteria. Nine studies demonstrated significant bacterial load reduction with phages compared to antibiotics alone; 20 did not demonstrate phage superiority, and 19 demonstrated possible synergistic and/or additive bacterial load reduction when phages were combined with antibiotics.
    CONCLUSION: Phages show promise as both monotherapy and adjunctive treatment for resistant MRSA infections, with phage-antibiotic combination therapy demonstrating superior outcomes in the majority of studies. Heterogeneity in infection models and phage selection limits direct comparisons across studies. No included studies explicitly addressed One Health. This highlights an important gap in the literature. While integration using a One Health framework is theoretically promising, further clinical investigations are needed to clarify the role of phage therapy within One Health strategies addressing AMR.
    Keywords:  Antimicrobial resistance; Bacteriophage therapy; MRSA; One health; Scoping review
    DOI:  https://doi.org/10.1007/s15010-026-02936-2
  4. Front Microbiol. 2026 ;17 1815573
      Methicillin-resistant Staphylococcus aureus (MRSA) remains one of the most significant multidrug-resistant bacterial pathogens responsible for a broad spectrum of infections ranging from mild skin infections to severe invasive diseases, including bacteremia, pneumonia, endocarditis, osteomyelitis, and sepsis. The rapid global dissemination of MRSA is primarily driven by the acquisition of the mecA gene encoding penicillin-binding protein 2a, which confers resistance to β-lactam antibiotics. In addition to β-lactam resistance, MRSA exhibits resistance to multiple antimicrobial classes through diverse mechanisms, including target-site mutations, efflux pumps, biofilm formation, horizontal gene transfer, and adaptive phenotypic variations. The virulence and persistence of MRSA is further enhanced by numerous virulence factors such as adhesins, toxins, immune evasion proteins, and extracellular enzymes that facilitate colonization, persistence, and host tissue damage. Biofilm formation additionally contributes to chronic infection and antibiotic tolerance. Despite the availability of conventional agents such as vancomycin, linezolid, and daptomycin, the emergence of resistant strains including vancomycin-resistant Staphylococcus aureus has significantly limited current therapeutic options. Consequently, there is an urgent need for innovative therapeutic strategies. This review comprehensively summarizes the evolution, pathogenesis, virulence mechanisms, biofilm biology, and antibiotic resistance mechanisms of MRSA, with particular emphasis on emerging therapeutic approaches. Novel strategies including antimicrobial peptides, nanomedicine, bacteriophage therapy, CRISPR-Cas systems, biomimetic nano-NETs, probiotics, monoclonal antibodies and plant-derived compounds are discussed as promising alternatives or adjuncts to conventional antibiotics. Collectively, these advances highlight the evolving landscape of MRSA management and the potential for next-generation therapeutics to overcome antimicrobial resistance challenges.
    Keywords:  CRISPR-Cas; PBP2a; biomimetic nano-NETs; mecA gene; methicillin-resistant Staphylococcus aureus
    DOI:  https://doi.org/10.3389/fmicb.2026.1815573
  5. Phage (New Rochelle). 2026 Sep;7(3): 187-196
       Background: Acinetobacter baumannii is a common bacterial pathogen in nosocomial infections. It has become one of the greatest threats to human health for its growing resistance to "last resort" antibiotics, which has led to a revival of phage therapy as a potential treatment. However, conventional methods for isolating A. baumannii-infecting phages are labor-intensive and often unsuccessful.
    Methods: Our approach involves a computational pipeline to identify temperate phages (prophages) integrated into A. baumannii genomes, followed by mitomycin C (MMC) induction of those strains to screen for active prophages.
    Results: Here we show a prophage analysis for nearly 900 A. baumannii genomes. We observed MMC-triggered excision of nine prophages from eight A. baumannii strains by Polymerase Chain Reaction (PCR) and sequencing. Further, we show four prophage-formed virions detectable by transmission electron microscopy and two that can plaque on other A. baumannii isolates.
    Conclusion: This work demonstrates the utility and diversity of prophages for further development as therapeutics for antibiotic-resistant A. baumannii.
    Keywords:  Acinetobacter baumannii; phage induction; phage therapy; prophages
    DOI:  https://doi.org/10.1177/26416549261461823
  6. Phage (New Rochelle). 2026 Sep;7(3): 178-186
       Background: Phage therapy is a promising alternative for drug-resistant Salmonella, but safety data for repeated doses are limited. This study investigated the sub-chronic safety of SE.PC, a candidate Salmonella enterica serovar Typhimurium phage cocktail, in a non-specific pathogen-free (non-SPF) model, which contains a naturally occurring microbiota, to evaluate systemic safety under physiologically relevant conditions. We tested a range of phage doses to determine if there are any differences in tolerance.
    Materials and Methods: During a 28-day repeated-dose oral toxicity study, SE.PC was administered via weekly gavage to male and female non-SPF BALB/c mice across three different phage doses (2.43 × 109, 4.85 × 109, and 9.70 × 109 plaque-forming units/mL). Clinical signs, body weight, hematology, biochemistry, and histopathology were assessed.
    Results: Across all three administered phage dose groups, no mortality was recorded, and all mice exhibited normal clinical signs and growth. Hematological and biochemical values remained within baseline ranges. Histopathology revealed no treatment-related lesions; isolated findings were deemed incidental or adaptive.
    Conclusion: Evidence supports that SE.PC is well tolerated. This study demonstrates a strong safety profile, supporting the advancement of SE.PC as a biocontrol agent.
    Keywords:  Salmonella enterica serovar Typhimurium; antimicrobial resistance; bacteriophage cocktail; phage therapy; preclinical safety; repeated-dose toxicity
    DOI:  https://doi.org/10.1177/26416549261448405
  7. Iran J Microbiol. 2026 Aug;18(4): 460-472
       Background and Objectives: This systematic review aimed to determine whether phage cocktails and co-evolutionarily trained phages prevent or delay the emergence of resistance in multidrug-resistant (MDR) bacteria more effectively than single-phage therapy in sewage and wastewater-derived systems, and to characterize the underlying mechanisms and the maturity of the current evidence base.
    Materials and Methods: Following PRISMA guidelines, a systematic review was performed using the PubMed and Scopus databases, with screening conducted via Rayyan Premium.
    Results: Among 4,795 records, 20 studies (≈0.4%) fulfilled the inclusion criteria; this low fraction reflects a deliberately broad search combined with strict eligibility criteria targeting original experimental studies of phage activity against MDR pathogens specifically isolated from sewage or wastewater. Phage cocktails showed a wider range of host coverage, a delay in resistance development, and improved penetration of biofilms, particularly when paired with depolymerases or antibiotics. Phage co-evolution training resulted in a more prolonged suppression of multidrug-resistant bacteria.
    Conclusion: Phage cocktails, especially those that are co-evolved and enhanced with enzymes, show potential for applications in wastewater; however, additional long-term and large-scale research is necessary.
    Keywords:  Anti-bacterial agents; Bacteriophages; Biofilms; Drug resistance; Klebsiella pneumoniae; Sewage; Wastewater
    DOI:  https://doi.org/10.18502/ijm.v18i4.22189
  8. Bull Math Biol. 2026 Aug 20. pii: 161. [Epub ahead of print]88(9):
      The antagonistic relationship between bacteria and bacteriophages (phages) drives genetic changes that result in phage resistance, making it important to understand the dynamics of resistance acquisition in both natural microbial communities and therapeutic contexts. We developed a generalized Lotka-Volterra model describing the interaction among phage-susceptible bacteria, phage-resistant bacteria, and a lytic phage, incorporating asymmetric interspecific competition and partial phage resistance. The model was fitted to growth data from four bacterial strains-Pseudomonas aeruginosa PA103 and PAK challenged with a phage cocktail, and Paenibacillus larvae Y-3650 and 25747 challenged with phage Fern IDv1-across four levels of multiplicity of infection (MOI). We compared two parameterization schemes: M1, in which the interspecific competition coefficients ars and asr are shared across MOI treatments, and M2, in which these coefficients are allowed to vary by treatment. For the two P. aeruginosa strains, M1 produced adequate fits (mean R2=0.91 and 0.89). For the two P. larvae strains, M1 failed catastrophically at specific treatments ( R2 as low as -19.7 ), while M2 achieved mean R2 of 0.90 and 0.95 for Y-3650 and 25747, respectively. Information-theoretic model comparison ( ΔAIC=-753 and -458 ) and profile likelihood analysis confirmed that the competition coefficients are fundamentally MOI-dependent, with per-treatment optima diverging by up to three orders of magnitude. These findings indicate an effect that extends beyond phage-mediated apparent competition-which assumes zero direct competition between the susceptible and resistant subpopulations-because the fitted interspecific competition coefficients are nonzero and MOI-dependent. We observed three distinct growth patterns-delayed growth, two-phase growth, and complete suppression-with non-monotonic dose-response relationships in which intermediate phage doses produced stronger suppression than higher doses. This non-monotonicity demonstrates that the boundaries between growth regimes are governed by competitive dynamics rather than phage dose alone. Our results establish that phage resistance evolution is fundamentally an ecological process: the competitive context, shaped by phage pressure, determines population-level outcomes independently of intrinsic mutation capacity. These findings have implications for phage therapy, suggesting that optimal dosing strategies must account for the competitive environment created by treatment, not solely the direct bactericidal effect.
    Keywords:  Bacteriophage resistance evolution; Competitive dynamics; Fitness costs of resistance; Generalized Lotka-Volterra (gLV); Multiplicity of infection; Ordinary differential equations; Phage-density-dependent competition; Profile likelihood
    DOI:  https://doi.org/10.1007/s11538-026-01704-5
  9. Smart Med. 2026 Aug;5(4): e70044
      Chronic diabetic foot ulcers represent a persistent clinical challenge characterized by a "locked" inflammatory phase, biofilm-mediated infection, and impaired tissue regeneration. Because conventional antibiotic and debridement therapies fail to resolve dysbiosis or stimulate healing, microbial antagonism has emerged as a potent biological principle for wound restoration. This narrative review integrates ecological evidence and mechanistic insights from animal models and early human studies to explore how beneficial microorganisms, including probiotics, bacteriophages, and competitive consortia, actively reshape diabetic wound environments. We examined the multifaceted mechanisms of these interactions, ranging from direct pathogen inhibition to host immunomodulation and metabolic signaling via conserved pathways such as the p40/epidermal growth factor receptor (EGFR)/PI3K axis. To address significant translational barriers, we introduce a "Regenerative Microbiology" framework that emphasizes spatiotemporal coordination and precision stratification. By tailoring the use of live biotherapeutics or metabolically independent postbiotics to a patient's vascular and microbial profiles, this approach offers a strategic roadmap for transforming the management of infection-driven tissue damage in chronic diseases.
    Keywords:  bacteriophages; diabetic wounds; dysbiosis; microbial antagonism; microbial ecology; microbiome therapy; probiotics; tissue regeneration
    DOI:  https://doi.org/10.1002/smmd.70044
  10. Front Microbiol. 2026 ;17 1851321
      The global spread of antimicrobial resistance (AMR) has intensified the search for alternatives to conventional antibiotics in animal production systems. Bacteriophages can be engineered beyond narrow-spectrum antibacterial agents into multifunctional biological platforms that integrate direct killing, immune modulation, and antigen delivery. We summarize recent advances across livestock, poultry, and aquaculture, delineating mechanistic distinctions between lytic phage therapy, phage display-derived interventions, and engineered platforms including CRISPR-Cas-enabled theranostic systems. However, as detailed below, most evidence remains preclinical, and translational gaps are substantial. Unlike prior descriptive reviews, we analyze translational bottlenecks-host range constraints, pharmacokinetic limitations, regulatory fragmentation-and assess the existing research evidence for claimed advantages such as microbiota preservation and biofilm penetration while upfront acknowledging inconsistent experimental outcomes and inherent application limitations behind these beneficial effects. We conclude that realizing phages' therapeutic potential in veterinary medicine requires coordinated progress in synthetic biology, scalable manufacturing, and regulatory harmonization within a One Health framework.
    Keywords:  animal health; antimicrobial resistance; engineered phage; mucosal immunity; phage therapy; phage vaccine; synthetic biology
    DOI:  https://doi.org/10.3389/fmicb.2026.1851321
  11. Endocrine. 2026 Aug 15. pii: 279. [Epub ahead of print]91(1):
       BACKGROUND: Diabetic foot ulcers (DFUs) arise from interacting clinical, microbial, and host processes, yet public datasets differ substantially in design, scale, and evidentiary strength.
    METHODS: We curated eight public DFU cohorts and defined an evidence hierarchy. PRJNA287759 was reprocessed from raw 16 S reads; outcome tests used one baseline sample per patient (74 healed and 15 non-healed/adverse). GSE134431 compared 13 DFU with 8 diabetic foot skin samples using limma. Host discrimination underwent fold-confined leave-one-sample-out validation, 100 repeated nested five-fold analyses, 200 label permutations, and independent rank-score evaluation in GSE80178.
    RESULTS: Baseline diversity and community structure did not differ by outcome (Shannon P = 0.159; Observed ASVs P = 0.669; PERMANOVA R2 = 0.0157, P = 0.142). Rothia was the only nominal genus (P = 0.0307), and none survived FDR correction across prevalence thresholds. GSE134431 yielded 2,873 differentially expressed genes with coherent epidermal repair enrichment. Host leave-one-sample-out AUC was 0.990 (95% CI 0.964-1.000), repeated nested-CV median AUC was 0.981 (95% interval 0.952-1.000), and permutation P was 0.00995. The GSE80178 score separated 6 DFU from 3 diabetic foot skin samples (exact P = 0.0238; AUC = 1.000), although this external comparison was small.
    CONCLUSIONS: Host expression provides the strongest evidence, whereas microbiome findings remain exploratory. The repair framework synthesizes parallel evidence without demonstrating causal coupling or a clinically deployable biomarker.
    Keywords:  16S rRNA; Clinical outcome; Diabetic foot ulcer; Epidermal repair; Host transcriptome; Microbiome; Multi-omics integration; Wound healing
    DOI:  https://doi.org/10.1007/s12020-026-04754-w
  12. Curr Oncol Rep. 2026 Aug 21. pii: 82. [Epub ahead of print]28(1):
       PURPOSE OF REVIEW: Oral potentially malignant disorders (OPMDs) comprise a heterogeneous group of lesions with a variable risk of progression to oral squamous cell carcinoma (OSCC). Although conventional risk assessment relies primarily on clinical and histopathological features, increasing evidence suggests that the local oral microenvironment may also influence malignant transformation. This narrative review examines the potential role of periodontitis as a modulator of OPMD progression, with particular emphasis on the underlying biological mechanisms and clinical implications. A targeted literature search was conducted in PubMed/MEDLINE, Scopus/EMBASE, and Web of Science for studies published between January 2000 and March 2026.
    RECENT FINDINGS: Available evidence suggests that periodontitis may promote a pro-tumorigenic oral microenvironment through the interconnected effects of chronic inflammation, microbial dysbiosis, and metabolic alterations. These processes may enhance epithelial proliferation, genomic instability, immune evasion, and field cancerization, potentially facilitating the progression of OPMDs to OSCC. Although direct longitudinal and interventional evidence remains limited, converging mechanistic, biological, and epidemiological findings support the hypothesis that periodontal disease may contribute to early oral carcinogenic processes. Periodontitis may represent an underrecognized component of oral cancer risk, particularly in patients with OPMDs. Incorporating periodontal assessment into their clinical management may support more comprehensive risk stratification. Although causality has not been established, controlling periodontal inflammation and dysbiosis may offer a potential preventive strategy. Further longitudinal and interventional studies are required to determine the clinical relevance of this association.
    Keywords:  Carcinogenesis; Chronic inflammation; Oral microbiome; Oral potentially malignant disorders; Oral squamous cell carcinoma; Periodontitis
    DOI:  https://doi.org/10.1007/s11912-026-01817-z
  13. J Appl Microbiol. 2026 Aug 17. pii: lxag204. [Epub ahead of print]
       AIMS: The convergence of multidrug resistance and hypervirulence in Klebsiella pneumoniae (MDR-HvKp) has narrowed treatment options. Despite growing interest in phage-antibiotic synergy (PAS), this study evaluates the underexplored combinatorial effects of phage and antibiotics, including drug-specific interactions and sequence dependency, against the biofilm-forming MDR-HvKp clinical strain.
    METHODS AND RESULTS: A T5-like Klebsiella bacteriophage, Round, within the genus Webervirus, was therapeutically and genomically characterized. A biofilm-forming clinical strain, Kleb_134, was used to evaluate in vitro phage-antibiotic interactions with meropenem, colistin, and tigecycline in planktonic and biofilm models.In planktonic assays, phage combinations with meropenem and colistin resulted in a multi-log CFU reduction compared to monotherapies, whereas reduced efficacy was observed with tigecycline. In biofilm assays, pre-phage treatment followed by antibiotic exposure demonstrated the strongest biofilm reduction. Drug-specific and sequence-dependent effects were evident. Meropenem-phage combinations reduced biofilm biomass by 2.85-fold (high phage titre) and 3.8-fold (low phage titre), while colistin-phage combinations achieved reductions of 8.4-fold (high phage titre) and 2.8-fold (low phage titre).
    CONCLUSIONS: Sequential phage-antibiotic treatment was effective against MDR-HvKp biofilms, with pre-phage exposure enhancing antibiotic access through biofilm disruption. The bacteriostatic nature of tigecycline reduced efficacy by affecting phage replication. These findings highlight the importance of treatment sequence and antibiotic selection, and extend existing knowledge in optimizing therapeutic outcomes in MDR-HvKp infections.
    Keywords:   Klebsiella pneumoniae, multidrug resistance; Biofilms; hypervirulence; phage-antibiotic combination; phages
    DOI:  https://doi.org/10.1093/jambio/lxag204
  14. Front Microbiol. 2026 ;17 1904655
      Ventilator-associated pneumonia remains one of the most common and severe healthcare associated infections in critically ill patients receiving mechanical ventilation. Among the predominant causative pathogens, multidrug-resistant Klebsiella pneumoniae and Acinetobacter baumannii pose a major clinical challenge due to their extensive antimicrobial resistance, environmental persistence, and ability to form biofilms on endotracheal tubes and other medical devices. Biofilm formation is central to VAP pathogenesis, facilitating bacterial adhesion, immune evasion, reduced antimicrobial penetration, and protective niches that support metabolic dormancy, persister-cell formation, and horizontal gene transfer. These mechanisms promote bacterial survival, recurrent infection, prolonged hospitalization, increased healthcare costs, and mortality. Resistance in K. pneumoniae and A. baumannii is mediated by multiple mechanisms, including β-lactamase production, efflux pump overexpression, reduced outer membrane permeability, target-site modification, and acquisition of mobile resistance determinants. The interaction between AMR and biofilm- associated persistence substantially compromises antimicrobial efficacy, particularly against carbapenem-resistant strains. Although recent advances have expanded treatment options, outcomes remain suboptimal in established biofilm-associated infections. This review examines the epidemiology, clinical burden, resistance mechanisms, biofilm mediated persistence, and host-pathogen interactions underlying MDR K. pneumoniae and A. baumannii associated VAP. Particular emphasis is placed on current therapeutic approaches and emerging anti-biofilm strategies, including antimicrobial-coated devices, nanotechnology-based interventions, matrix-disrupting enzymes, antimicrobial peptides, and anti-virulence approaches targeting quorum sensing and biofilm maturation. Understanding the interplay between AMR, biofilm persistence, and therapeutic failure is critical for developing more effective strategies to prevent and manage MDR-VAP.
    Keywords:  Acinetobacter baumannii; Klebsiella pneumoniae; anti-biofilm therapeutics; biofilm-mediated persistence; ventilator-associated pneumonia
    DOI:  https://doi.org/10.3389/fmicb.2026.1904655
  15. World J Nephrol. 2026 Sep 25. 15(3): 118797
      Multidrug-resistant (MDR) bacterial infections are a major cause of morbidity, mortality, and graft loss in kidney transplant recipients, who are particularly vulnerable due to intensive immunosuppression, frequent healthcare exposure, and recurrent antibiotic use. The rising prevalence of resistance among key uropathogens and bloodstream isolates has outpaced the development and uptake of transplant-specific diagnostic and therapeutic strategies. This mini-review synthesizes contemporary evidence on MDR gram-negative and gram-positive infections in kidney allograft recipients, focusing on extended-spectrum beta-lactamase-producing Enterobacterales, carbapenem-resistant Enterobacterales, difficult-to-treat Pseudomonas aeruginosa, carbapenem-resistant Acinetobacter baumannii, vancomycin-resistant Enterococci, methicillin-resistant Staphylococcus aureus, and Clostridioides difficile infection. We outline epidemiology, risk factors, and clinical impacts on patient and graft outcomes, including the association of recurrent urinary tract infection and MDR bloodstream infection with reduced estimated glomerular filtration rate, increased hospitalization, and higher death-censored graft failure. The article highlights advances in rapid molecular diagnostics (such as polymerase chain reaction, matrix-assisted laser desorption/ionization-time of flight, and syndromic panels) that shorten time to organism and resistance detection, enabling earlier optimization and de-escalation of antimicrobial therapy. Emerging agents - including novel beta-lactam/beta-lactamase inhibitor combinations, siderophore cephalosporins, and investigational boronate beta-lactamase inhibitors - are discussed, emphasizing nephrotoxicity profiles, drug-drug interactions with calcineurin inhibitors, and evidence gaps in transplant populations. We also review the evolving roles of phage therapy, monoclonal antibodies, vaccines, and therapeutic drug monitoring (including area under the curve/minimum inhibitory concentration-guided vancomycin dosing and cautious reuse of aminoglycosides) in this high-risk cohort. Finally, we propose a pragmatic stewardship framework tailored to kidney transplant units, integrating individualized perioperative prophylaxis, avoidance of unnecessary treatment of asymptomatic bacteriuria, early device removal, and minimization of broad-spectrum exposure to limit MDR selection and Clostridioides difficile infection. A transplant-specific approach that combines rapid diagnostics, rational deployment of new antimicrobials, and rigorous stewardship is essential to improve survival and preserve allograft function in the era of MDR infections after kidney transplantation.
    Keywords:  Antimicrobial stewardship; Bloodstream infection; Kidney allograft recipients; Kidney transplantation; Molecular rapid diagnostics; Multidrug-resistant bacterial infections; Novel antimicrobial agents; Therapeutic drug monitoring; Urinary tract infection
    DOI:  https://doi.org/10.5527/wjn.118797
  16. Diabetes Metab Res Rev. 2026 Sep;42(6): e70219
       AIMS: The social drivers of diabetic foot disease (SDDFD) impact ulcer incidence and outcomes, but how is relatively unknown. We aimed to build a conceptual model positing how three SDDFD-poverty, insurance, and government assistance-influence outcomes for patients with diabetic foot ulcers.
    MATERIALS AND METHODS: To inform the model, we conducted a scoping review of studies linking poverty, insurance, and/or government assistance with diabetic foot ulcer outcomes using Levac and colleagues' 6-stage framework, the Preferred Reporting Items for Systematic Review and Meta-analysis extension for scoping reviews, and the SPIDER tool. We searched PubMed, CINAHL, and Embase for original research articles set in the United States and published between 1 January 2005 and 7 November 2025. We excluded those published in a language other than English, case reports, abstracts, and studies of other SDDFD.
    RESULTS: Data from 35 included studies informed our conceptual model, depicting a complex causal pathway between SDDFD and outcomes, often mediated by healthcare system factors and moderated by patients' abilities to adhere to healthcare recommendations. Multidisciplinary teams might ameliorate the risk of amputations associated with poverty, lack of insurance, or qualifying for government assistance. Additionally, the model highlights reinforcing feedback loops, both positive and negative.
    CONCLUSION: Our model can be used to inform mediation and moderation analyses and help avoid collider-conditioning bias during statistical analyses. It can help identify potential points of intervention within the healthcare system for those working to dampen the negative effects of SDDFD on outcomes.
    Keywords:  amputation; causal pathway; diabetic foot ulcer; limb salvage; mediation; moderation; social drivers of health
    DOI:  https://doi.org/10.1002/dmrr.70219
  17. Respir Med. 2026 Aug 19. pii: S0954-6111(26)00472-5. [Epub ahead of print] 109104
      Antimicrobial resistance (AMR) represents one of the most pressing threats to global public health, undermining the effectiveness of modern antimicrobial therapy and challenging decades of medical progress. This comprehensive review examines the transition from broad-spectrum empirical therapy toward precision medicine as an integrated framework for improving antimicrobial use and combating AMR. Precision medicine seeks to tailor treatment decisions by combining pathogen-specific genomic and resistance data with relevant host characteristics to optimize therapy while limiting unnecessary antimicrobial exposure and the selective pressures that drive resistance. The review synthesizes advances reported from 2020, highlighting established and emerging approaches including rapid molecular diagnostics, next-generation sequencing, CRISPR-based detection, machine learning (ML)-assisted decision support, precision dosing, and targeted therapeutics such as bacteriophage therapy, antimicrobial peptides, and bacterial proteolysis-targeting chimeras. Rather than functioning as isolated technologies, these approaches achieve their greatest clinical value when integrated within antimicrobial stewardship programs and a One Health framework that recognizes the interconnected human, animal, and environmental drivers of resistance. Despite considerable progress, important challenges remain, including equitable access to advanced technologies, interpretation of increasingly complex datasets, workforce and infrastructure limitations, and evolving regulatory pathways for novel diagnostics and therapeutics. This review concludes that while precision medicine is not a standalone solution, its successful implementation will depend on coordinated integration of diagnostics, host factors, computational tools, pharmacological optimization, and stewardship strategies to improve patient outcomes while preserving the long-term effectiveness of existing antimicrobials.
    Keywords:  Antimicrobial Resistance; Artificial Intelligence; Novel Therapeutics; Precision Medicine; Rapid Diagnostics
    DOI:  https://doi.org/10.1016/j.rmed.2026.109104
  18. Int J Low Extrem Wounds. 2026 Aug 18. 15347346261479522
      Recurrence of diabetic foot ulcer (DFU) remains a major clinical challenge in patients with diabetes. Therapeutic footwear is a cornerstone of secondary prevention as it reduces plantar pressure and mechanical stress. However, most available evidence focuses on traditional orthopedic footwear, while prospective data on therapeutic sport footwear designed for daily physical activities are poor. This study aimed to evaluate the effectiveness of therapeutic sport footwear with a semi-rigid biomechanical sole (termed YDA) with combined insole in preventing ulcer recurrence in patients with diabetes at high risk of re-ulceration. The study was a single-arm, uncontrolled, retrospective observational study including consecutive patients with diabetes and a history of DFU referred to a tertiary diabetic foot center between January 2024 and March 2025. All included patients wore YDA as therapeutic footwear during the study period. In addition, they were managed through a multidisciplinary protocol including regular clinical follow-up, risk-factor monitoring, and structured patient education. The 1-year outcomes included: ulcer recurrence, minor and major amputations. Overall, 47 patients were included (68.1% male; mean age 68.3 ± 8.4 years). Peripheral neuropathy was present in all patients and peripheral arterial disease in 55.3%. At 1 year of follow-up, ulcer recurrence occurred in 14.9% of patients, minor amputation in 6.4%, and no major amputations were recorded. The multivariate analysis showed that discontinuous use of therapeutic footwear was strongly associated with ulcer recurrence (OR 8.9, 95% CI 2.1-37.5; p < 0.0001) and minor amputation (OR 10.5, 95% CI 2.0-55; p = 0.004). Peripheral arterial disease was independently associated with minor amputation (OR 5.2, 95% CI 1.3-20.6; p = 0.02). The use of specific sport footwear with a semi-rigid biomechanical sole resulted in a low rate of re-ulceration.
    Keywords:  diabetic foot; diabetic foot ulcer; therapeutic footwear; ulcer recurrence
    DOI:  https://doi.org/10.1177/15347346261479522
  19. J Periodontal Res. 2026 Aug 17.
      Chronic liver diseases and periodontitis are prevalent inflammatory disorders that impose substantial global health and economic burdens. Increasing evidence supports an oral-hepatic axis through which periodontal dysbiosis and chronic oral inflammation may influence hepatic homeostasis and disease progression. Epidemiologic studies have linked periodontitis with metabolic dysfunction-associated steatotic liver disease (MASLD), nonalcoholic fatty liver disease (NAFLD), viral hepatitis, cirrhosis, and hepatocellular carcinoma. Although many associations persist after adjustment for shared risk factors, including obesity, diabetes, smoking, nutritional status, and socioeconomic factors, the independent contribution of periodontitis to liver disease remains unclear. Experimental and translational studies indicate that periodontal pathogens and their virulence factors promote systemic inflammation, endotoxemia, microbial translocation, immune dysregulation, and alterations in gut microbial ecology. Nutritional factors may further influence these interactions through their effects on host immunity, inflammation, and microbial communities. Together, these mechanisms converge along the oral-gut-liver axis to activate hepatic inflammatory, oxidative stress and profibrotic pathways that contribute to steatosis, immune activation, and fibrogenesis. Emerging evidence suggests that trained immunity, driven by metabolic and epigenetic reprogramming, may represent an additional mechanism linking periodontitis and liver disease warranting future studies. Preliminary studies indicate that periodontal therapy may reduce systemic inflammatory burden and improve hepatic biomarkers; however, evidence for an effect on liver disease progression or clinical outcomes remains limited. Similarly, hepatic dysfunction may exacerbate immune dysregulation and periodontal breakdown, reinforcing an inflammatory loop. However, overall causal relationships remain to be established. This review summarizes current epidemiological, clinical, and mechanistic evidence linking periodontitis and chronic liver diseases, highlighting the oral-gut-liver axis, shared immunometabolic and nutritional pathways. Understanding these interactions shows the significance of integrated dental-medical care and opens new avenues for prevention and adjunctive therapy to sustain oral and hepatic health.
    Keywords:  cirrhosis; dysbiosis; hepatitis; hepatocarcinoma; metabolic dysfunction‐associated steatotic liver disease; nonalcoholic fatty liver disease; obesity; oral‐gut‐liver axis; periodontitis
    DOI:  https://doi.org/10.1111/jre.70159
  20. Lancet Microbe. 2026 Aug 19. pii: S2666-5247(26)00125-4. [Epub ahead of print] 101470
       BACKGROUND: The PhagoBurn trial compared the efficacy and tolerability of a cocktail of lytic anti-Pseudomonas aeruginosa bacteriophages with standard of care for patients with burns but did not meet its primary endpoint. In this follow-up study using samples from the PhagoBurn trial, we aimed to investigate non-canonical phage-bacterium interactions and longitudinal changes in the phenotypic and genotypic characteristics of P aeruginosa during phage therapy.
    METHODS: In this retrospective, observational, reverse translational study, we collected viable P aeruginosa isolates from the wound swabs of individuals who completed at least 7 days of treatment with the phage cocktail product (PP1131) in the multicentre PhagoBurn trial conducted in Switzerland, France, and Belgium. Isolates were subjected to longitudinal phenotypic screening for phage susceptibility using standard drop assays and reverse double-layer assays. Isolates with unique phage susceptibility profiles were selected for genomic characterisation. After the unexpected detection of PP1131-derived phage DNA in many bacterial isolates, non-canonical phage-bacterium interactions were assessed using double-layer agar lysis assays and ultrastructural assessments using electron microscopy.
    FINDINGS: Of the 27 individuals enrolled in the PhagoBurn trial, 13 received phage therapy; among them, ten individuals completed the therapy, and longitudinal P aeruginosa isolates from seven individuals were available for analysis. 184 (mean 26 [SD 21·9] isolates per individual) bacterial isolates were phenotypically assessed. Phage susceptibility varied substantially between and within individuals over time, with rapid emergence of resistance in some cases. 98 (53%) of 184 isolates (mean 14 [11·8] isolates per individual) had unique phage susceptibility profiles and were selected for genome sequencing. In these 98 isolates, mutations were detected in genes involved in virulence regulation, secretion and efflux, chemotaxis, and biosynthesis. Complete or partial phage genomes derived from PP1131 were detected in 41 (42%) of the 98 sequenced bacterial isolates; 16 (39%) of 41 phage-positive isolates continuously secreted infectious phage particles, consistent with a productive chronic infection phenotype. In 23 (56%) of 41 isolates, phage genomic DNA persisted without detectable phage release, suggesting non-productive chronic infection (including putative pseudolysogeny). These alternative infection states remained stable and detectable after serial passages in vitro and were observed in isolates from individuals with both favourable and unfavourable clinical outcomes.
    INTERPRETATION: Bacterial adaptation, phage adaptation, and non-canonical phage-bacterium interactions have been largely overlooked in clinical trial design and might contribute to variability in therapeutic outcomes and warrant further investigation. The non-canonical phage interactions (eg, chronic infection and pseudolysogeny) observed in this study highlight the need for protocols that account for the emergence of persistent non-lytic phage-bacterium interactions.
    FUNDING: Stiftung für die Forschung in Anästhesiologie und Intensivmedizin, Swiss National Science Foundation, and Placide Nicod Foundation.
    DOI:  https://doi.org/10.1016/j.lanmic.2026.101470
  21. Sci Rep. 2026 08 19. pii: 26054. [Epub ahead of print]16(1):
      We identified and characterized a novel lytic phage, HMGUpa1, which exhibited distinct infection efficiency across five characterized P. aeruginosa clinical isolates. While all strains were susceptible to the phage, killing efficacy varied significantly, with strain 50071 showing the highest sensitivity. To explore the basis of this variability, we analyzed phage infection dynamics, host genomic features, and transcriptomic responses. Strain-specific differences in gene content and transcriptional profiles, particularly those related to virulence-associated secretion systems, RNA repair, and metabolic reprogramming, were associated with differences in phage efficacy. These findings suggest that differences in phage efficacy arise from a complex interplay of strain-specific molecular, physiological, and infection-related host traits. Together, our results highlight the importance of considering host-specific traits when developing precision-based phage therapy strategies against multidrug-resistant P. aeruginosa.
    DOI:  https://doi.org/10.1038/s41598-026-64067-w
  22. Microbiol Spectr. 2026 Aug 20. e0078226
      Antimicrobial resistance is a major threat to human health. Phage therapy is a promising alternative to antibiotics, yet routine diagnostic tools capable of rapidly determining phage susceptibility are lacking. Our goal was to develop a diagnostic tool providing easy-to-interpret in vitro phage activity results within a clinically relevant time frame. We developed a prototype based on bioluminescence emitted by adenosine triphosphate (ATP) release during phage-mediated bacterial lysis. Ten phages were tested against 20 clinical isolates of four species: Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae. Overall concordance with double agar overlay (DAO) was fair (72%). The prototype showed high sensitivity (94.5%) at detecting weak killers and high specificity (87%) at identifying strong killer phages. Most discordances involved DAO weak killers classified as active by the prototype. Most of these discordant phage-bacteria couples produced a spot in the spot test, suggesting the assay may detect but not distinguish killing without replication from killing with replication. The method showed high repeatability and reproducibility. A few aberrant species specificity results highlighted the need for routine controls. Results from prospective urine samples were obtained within an average of 5 h.
    IMPORTANCE: The lack of reproducible user-friendly phage susceptibility testing that can timely inform clinical decisions remains a major barrier to the routine clinical use of phages. We developed a high-throughput, semi-automated prototype that delivers easy-to-interpret phage susceptibility results for four bacterial species within 5 h in a "real-life" clinical flow. However, the correlation between in vitro lytic activity and clinical outcomes still needs to be established to meet regulatory requirements for in vitro diagnostic (IVD) medical devices.
    Keywords:  bacteriophages; clinical microbiology; phage susceptibility testing
    DOI:  https://doi.org/10.1128/spectrum.00782-26
  23. Methods Mol Biol. 2026 ;3055 179-187
      Fusobacterium nucleatum is a Gram-negative, anaerobic bacterium that naturally colonizes the human oral cavity and is recognized as an etiological agent in both local and systemic diseases, including periodontitis, preterm birth, and colorectal cancer. To advance research into the systemic impact of oral-origin F. nucleatum-associated pathologies, it is crucial to optimize the use of animal models. In this study, we present a detailed protocol for establishing a mouse model of experimental periodontitis through oral inoculation of bacteria, including F. nucleatum. This model provides a valuable platform for investigating the pathogenic mechanisms and systemic consequences of oral bacterial infections.
    Keywords:  Alveolar bone loss; Fusobacterium nucleatum; Ligature; Mouse model
    DOI:  https://doi.org/10.1007/978-1-0716-5388-3_17
  24. Geriatr Nurs. 2026 Aug 21. pii: S0197-4572(26)00481-7. [Epub ahead of print]73 104276
      Diabetes and frailty are two interrelated health conditions that mutually exacerbate one another and have a detrimental impact on quality of life in older adults. Frailty, characterised by depleted physiological reserves, cognitive decline and impaired self-care capacity, complicates diabetes management. Consequently, this population faces an elevated risk of serious foot-related complications, including diabetic foot ulcer development and delayed wound healing. This narrative review evaluates the pathophysiology, epidemiology and nursing interventions for foot-health issues in frail older adults with diabetes. A literature review was conducted using the keywords 'frailty', 'diabetes', 'diabetic foot', 'self-management' and 'nursing interventions' in the PubMed, Scopus and Google Scholar databases. Recent studies, clinical guidelines and relevant reviews were prioritised for inclusion in the assessment, particularly those addressing frailty and diabetic foot care in conjunction. Studies indicate that frailty is associated with the development of diabetic foot ulcers, delayed wound healing and reduced adherence to foot care. Effective nursing interventions encompass more than just standard wound care; they also include early and proactive risk assessment, frailty screening, self-management support tailored to cognitive and functional status, caregiver involvement, behaviour change approaches and multidisciplinary collaboration. Furthermore, technology-enabled monitoring systems and digital health applications offer significant opportunities to improve care adherence and the early detection of complications in this high-risk group. In conclusion, comprehensive nursing approaches tailored to the unique needs of frail older adults with diabetes are critical for reducing complications and improving quality of life in this vulnerable population.
    Keywords:  Diabetes mellitus; Diabetic foot; Foot health; Frailty; Nursing interventions
    DOI:  https://doi.org/10.1016/j.gerinurse.2026.104276
  25. J Comp Eff Res. 2026 Aug 18. e260063
      Aim: To compare the healthcare resource utilization, spending and clinical outcomes associated with porcine placental extracellular matrix (PPECM [InnovaMatrix® AC, Convatec Triad Life Sciences, LLC, TN, USA]) versus standard of care (SOC) and other advanced treatments (AT) among Medicare Fee-For-Service beneficiaries with diabetic foot ulcers (DFU) or venous leg ulcers (VLU). Materials & methods: A retrospective cohort study was conducted using 100% Medicare Parts A and B administrative claims from October 2021 through December 2024. Treatment episodes for DFU and VLU were identified and categorized as PPECM plus SOC, SOC alone or AT plus SOC. Patients were followed for 6 months after the end of each treatment episode. Outcomes included post-episode healthcare utilization by site of care, per-patient-per-month spending, wound-related complications and amputations. Results: The final study sample included 109,786 eligible DFU patients (117,633 episodes of care; PPECM 0.2%, SOC 95.0%, AT 4.8%) and 53,624 eligible VLU patients (56,498 episodes of care; PPECM 0.1%, SOC 95.5%, AT 4.4%). In DFU, PPECM was associated with significantly lower post-episode utilization in most sites of care compared with SOC, and fewer wound complications including amputations compared with AT. Although total post-episode costs were higher for PPECM than SOC and AT in DFU, PPECM had lower overall cost at specific types of care sites. In VLU, PPECM was associated with significantly fewer visits across most sites of care compared with AT and SOC. PPECM-treated VLU episodes had significantly lower total costs than both SOC and AT. Rates of wound complications and amputations were similar across treatment groups in VLU. Conclusion: Treatment with PPECM demonstrated lower overall post-episode costs compared with SOC and other AT while maintaining safety outcomes, particularly in VLU, suggesting potential value in chronic wound management.
    Keywords:  CAMPS; CMS; Center for Medicare and Medicaid Services; DFU; ECM; VLU; cellular acellular matrix-like products; diabetic foot ulcer; diabetic ulcer; extracellular matrix; hard-to-heal wounds; health economics; retrospective study; venous leg ulcer; wound care
    DOI:  https://doi.org/10.57264/cer-2026-0063
  26. Front Cell Infect Microbiol. 2026 ;16 1935103
      
    Keywords:  One Health; antimicrobial resistance (AMR); antimicrobial stewardship; clinical microbiology; genomic surveillance; infection prevention
    DOI:  https://doi.org/10.3389/fcimb.2026.1935103
  27. Front Microbiol. 2026 ;17 1853113
      Bacterial resistance to bacteriophages represents a major limitation for the durable use of phage-based biocontrol strategies in agriculture. Here, we used a phage-resistance derivative of Xanthomonas campestris pv. campestris (Xcc) as a selective host to isolate an additional phage with activity against resistant bacteria. A spontaneous mutant, designated Xcc 8004R, was isolated following exposure of Xcc 8004 to phage X1. Xcc 8004R exhibited a growth profile comparable to that of the wild-type strain, but differed in colony appearance, X1 adsorption efficiency, and motility phenotypes. Whole-genome resequencing identified mutations in genes annotated as encoding a lipopolysaccharide (LPS) core biosynthesis protein, PilY1, N-acetylmuramoyl-L-alanine amidase, and IS1404 transposase, and a conserved hypothetical protein. Using Xcc 8004R as the isolation host, we recovered a bacteriophage, vB_Xcc_GYRb1 (GYRb1), from agricultural soil. GYRb1 infected both Xcc 8004 and Xcc 8004R and exhibited different adsorption kinetics on the two strains. Transmission electron microscopy showed an icosahedral head and a long, non-contractile tail. Genome sequencing revealed a circularly assembled 91,478-bp double-stranded DNA genome encoding 128 predicted open reading frames and two tRNAs. No recognizable antibiotic resistance or bacterial virulence genes were detected. Phylogenomic, ANI, and gene-sharing network analyses suggest that GYRb1 is highly divergent from currently available related phages and may represent a candidate genus-level lineage within the class Caudoviricetes. GYRb1 suppressed the growth of Xcc 8004 and Xcc 8004R in vitro and reduced disease severity in cabbage leaf. A cocktail containing GYRb1 and X1 reduced OD600-based bacterial regrowth compared with single-phage treatments in vitro. Together, these findings support the feasibility of using phage-resistant bacterial mutants as selective hosts to obtain complementary candidate phages, while highlighting the need for receptor identification, resistance-frequency analysis, lysogeny assessment, and broader host-range testing before practical application.
    Keywords:  Caudoviricetes; Xanthomonas campestris pv. campestris; bacteriophage; biocontrol; phage adsorption; phage cocktail; phage resistance
    DOI:  https://doi.org/10.3389/fmicb.2026.1853113
  28. Int Microbiol. 2026 Aug 15.
    TÜBİTAK-121Z447 project scholars and consultants
      The objectives of this study were to isolate, characterize, and evaluate the biocontrol potential of Staphylococcus aureus-targeting bacteriophages in milk. From wastewater and raw milk samples, a total of 19 lytic phages were isolated. Among them SAKAY1298 and SAKAY1306 phages were selected for detailed phenotypic and genomic analysis based on their lytic profiles, host range and growth kinetics. Both phages exhibited icosahedral capsids with short, non-contractile tails under transmission electron microscopy. The phages exhibited moderate acid tolerance by remaining stable at pH 4 and maintained thermal stability at temperatures up to 50 °C. They also maintained high titers over one year at + 4 °C and -80 °C in tryptic soy broth and Tris-buffered saline (1 ×). Genome analyses revealed that both phages possess complete genomes (~ 17.5 kb), encoding 18 phage-related genes and lacking virulence and resistance determinants. Comparative and phylogenetic analyses confirmed assignment to the genus Rosenblumvirus, with 99% query coverage and ~ 92% whole-genome nucleotide identity to Staphylococcus phage vB_SauR_SW21 (GenBank accession OR683639.1). Significant reductions in S. aureus counts were achieved following phage cocktail treatment, reaching up to 4.31 log CFU/mL in vitro at 37 °C and 1.44 log CFU/mL in UHT milk at 4 °C (p < 0.001). Additionally, the phage cocktail fully inhibited the production of staphylococcal enterotoxin A in the in vitro assay. Altogether, the data supports the application of SA1298KAY and SA1306KAY phages have strong potential as effective biocontrol agents for reducing S. aureus in dairy applications.
    Keywords:  Biocontrol; Long-term storage; Staphylococcal enterotoxin; TEM; UHT milk; Whole-genome sequencing
    DOI:  https://doi.org/10.1007/s10123-026-00862-9
  29. Phys Rev E. 2026 Jul;114(1-1): 014412
      Bacteriophage-bacteria interactions are central to microbial ecology, influencing evolution, biogeochemical cycles, and pathogen behavior. Most theoretical models assume static environments and passive bacterial hosts, neglecting the joint effects of bacterial traits and environmental fluctuations on coexistence dynamics. This limitation hinders the prediction of microbial persistence in dynamic ecosystems such as soils and oceans. Using a minimal ordinary differential equation framework, we demonstrate that environmental fluctuations can suppress destructive oscillations through resonance, promoting coexistence where static models otherwise predict collapse. Counterintuitively, we find that lower bacterial growth rates are helpful in enhancing survival under high infection pressure, elucidating the observed postinfection growth reduction. Our studies highlight bacterial hosts as active builders of ecological dynamics and environmental variation as a potential stabilizing force. Our findings thus bridge a theory-experiment gap and provide a framework for predicting microbial responses to environmental stress, which might have potential implications for phage therapy, microbiome management, and climate-impacted community resilience as well.
    DOI:  https://doi.org/10.1103/rch2-2hsr
  30. J Breath Res. 2026 Aug 19.
       BACKGROUND: Cystic fibrosis (CF) is a genetic disorder characterized by chronic airway infection and progressive lung damage. Early identification of biomarkers associated with respiratory pathogens and inflammatory processes is crucial for improving disease monitoring and guiding therapy. Analysis of volatile organic compounds (VOCs) in exhaled breath has emerged as a promising non-invasive approach for biomarker discovery.&#xD;Objective: This systematic review aimed to identify and summarize VOCs detected in the exhaled breath of patients with cystic fibrosis that may serve as potential clinical biomarkers. &#xD;Methods: A systematic literature search was conducted in accordance with PRISMA guidelines across MEDLINE, Web of Science, SCOPUS, and Google Scholar, covering publications from 2000 to 2026. Studies comparing VOC profiles in patients with cystic fibrosis and control groups were included. The risk of bias in the included studies was assessed using the Newcastle-Ottawa Scale.&#xD;Results: Out of 431 identified records, 27 studies met the inclusion criteria and were included in the qualitative synthesis. These studies employed a variety of analytical platforms, including gas chromatography-mass spectrometry (GC-MS), selected-ion flow-tube mass spectrometry (SIFT-MS), proton transfer reaction mass spectrometry (PTR-MS), and nuclear magnetic resonance (NMR) metabolomics. Across all studies, a total of 121 VOCs were reported in association with cystic fibrosis. Several compounds, including hydrogen cyanide, 2-aminoacetophenone, pentane, and dimethyl sulfide, were repeatedly identified and may represent potential biomarkers related to bacterial colonization, oxidative stress, and inflammatory processes. However, substantial heterogeneity was observed among studies with respect to study design, patient populations, breath sampling protocols, and analytical methodologies.&#xD;Conclusion: Breath analysis based on volatile organic compounds represents a promising non-invasive approach for identifying biomarkers of cystic fibrosis and associated respiratory infections. Nevertheless, future progress in breathomics will primarily depend on improving methodological rigor rather than limiting the diversity of analytical platforms. Standardized breath sampling protocols, rigorous preprocessing workflows for mass spectrometry data, sufficiently powered multicenter studies, and external validation in independent patient cohorts are essential prerequisites for the clinical implementation of breath-based biomarkers.
    Keywords:  biomarkers; breath analysis; cystic fibrosis; exhaled breath; mass spectrometry; metabolomics; volatile organic compounds
    DOI:  https://doi.org/10.1088/1752-7163/ae9be1
  31. Front Cell Infect Microbiol. 2026 ;16 1820893
       Objective: To investigate the regulatory effect of the hospital preparation Golden Wanhong Ointment on wound microbiota structure in patients with diabetic foot ulcer (DFU), to provide microbiological evidence for its traditional functions of "clearing heat and detoxifying, removing necrotic tissue and promoting granulation."
    Methods: A total of 31 DFU patients were enrolled, and 62 wound secretion samples were collected before and after treatment. 16S rRNA gene V3-V4 region amplicon sequencing was performed to analyze changes in microbiota composition and diversity. Wound area, pain score (VAS), and inflammatory factors (IL-6, TNF-α) were measured before and after treatment.
    Results: Compared to pre-treatment, post-treatment assessments showed significantly reduced wound area, lower pain scores, and decreased levels of IL-6 and TNF-α (all P<0.001). Sequencing yielded 4,579,390 high-quality Clean Reads, with an average of 73,861 high-quality reads per sample. Microbiota analysis revealed a significant compositional remodeling. of wound microbiota structure after treatment: at the phylum level, the relative abundance of Proteobacteria decreased while Firmicutes increased; at the genus level, the abundances of Prevotella and Escherichia-Shigella, which are closely associated with infection, were significantly reduced.
    Conclusion: Golden Wanhong Ointment may promote wound healing by regulating DFU wound microbiota structure, inhibiting the abundance of key pathogenic bacteria, reducing local inflammatory response, and improving the wound microenvironment. This study provides a modern scientific interpretation of its traditional effects from a microbiological perspective.
    Keywords:  16S rRNA sequencing; Jinwanhong ointment; diabetic foot ulcers; microbiome; wound microbiota
    DOI:  https://doi.org/10.3389/fcimb.2026.1820893
  32. Biomol Biomed. 2026 Aug 12.
      Stenotrophomonas maltophilia is an opportunistic, multidrug-resistant (MDR), Gram-negative bacterium that has emerged as an important cause of hospital- and community-acquired infections, particularly in immunocompromised, critically ill, and hospitalized patients. Its increasing clinical relevance is driven by extensive virulence determinants, biofilm formation, intrinsic and acquired antimicrobial resistance, and persistence within healthcare-associated environmental reservoirs. This narrative review summarizes current evidence on the epidemiology, pathogenic mechanisms, antimicrobial resistance, and therapeutic management of S. maltophilia, with a particular focus on emerging treatment strategies. A literature search of PubMed, Scopus, Web of Science, and Google Scholar was performed to identify relevant studies published between 1997 and 2026. The available evidence demonstrates that biofilm formation, quorum sensing, adhesion factors, secretion of extracellular enzymes, and multiple resistance mechanisms-including β-lactamases, multidrug efflux pumps, and horizontally acquired resistance genes-substantially contribute to persistent infections and therapeutic failure. Hospital outbreaks are frequently associated with contaminated water systems, sinks, and medical devices, emphasizing the importance of environmental surveillance and infection control. Trimethoprim-sulfamethoxazole (TMP-SMX) remains the recommended first-line therapy, whereas minocycline, tigecycline, fluoroquinolones, and cefiderocol represent important alternatives for selected patients. Emerging approaches, including novel tetracyclines, bacteriophages, bacteriocins, ceragenins, anti-biofilm agents, and plant-derived compounds, have demonstrated promising preclinical activity against MDR and biofilm-associated infections, although robust clinical evidence remains limited. Continued surveillance, antimicrobial stewardship, pharmacokinetic/pharmacodynamic-guided therapy, and well-designed prospective clinical studies are essential to optimize the management of S. maltophilia infections and facilitate the translation of emerging therapeutic strategies into clinical practice.
    DOI:  https://doi.org/10.17305/bb.2026.14293
  33. Trends Microbiol. 2026 Aug 17. pii: S0966-842X(26)00212-X. [Epub ahead of print]
      Shiga toxin-producing Escherichia coli (STEC) presents a therapeutic dilemma because antibiotic treatment induces prophage-encoded Shiga toxin expression. Galtier et al. engineer phage-derived particles with programmable cell specificity, genetic cargo, and CRISPR guide RNAs that destroy stx while killing STEC in animal models.
    Keywords:  CRISPR; gut microbiome; phage delivery; phage engineering; phage therapy
    DOI:  https://doi.org/10.1016/j.tim.2026.07.013
  34. Microbiol Spectr. 2026 Aug 20. e0135826
      Pseudomonas aeruginosa airway colonization and infection are major drivers of chronic obstructive pulmonary disease (COPD) acute exacerbations, yet its adaptive evolution in the COPD airway remains poorly understood. Here, six serial P. aeruginosa strains were isolated over a 5-month interval from a single COPD patient: two initial isolates (SCPa03-1 and SCPa03-2) from one sputum sample and four subsequent isolates (SCPa12-1, SCPa12-2, SCPa12-3, and SCPa12-4) from another. Of them, SCPa03-1, SCPa12-1, and SCPa12-2 exhibited a non-mucoid phenotype, while SCPa03-2, SCPa12-3, and SCPa12-4 were mucoid. All isolates were resistant to most β-lactam antibiotics, including imipenem, but susceptible to amikacin and gentamicin. Notably, three mucoid strains were susceptible to meropenem. Genomic and phylogenetic analysis revealed that all isolates were clonally related, belonged to ST274, and were closely related to the globally prevalent high-risk ST274 clone. Sequence analysis and qRT-PCR suggested that their imipenem resistance may be associated with ampC overexpression and oprD mutations, while reduced ciprofloxacin susceptibility likely stemmed from gyrA/gyrB mutations and acquisition of crpP-harboring ICEs. Despite a shared mucA frameshift mutation, secondary mutations in prc (SCPa03-1) and algU (SCPa12-1, SCPa12-2) likely accounted for the non-mucoid phenotype. Relative to PAO1, all isolates showed reduced growth, diminished pyoverdine, attenuated Galleria mellonella virulence, and increased pyocyanin. Besides, three mucoid strains exhibited slower growth, reduced serum resistance, and attenuated virulence compared with non-mucoid counterparts, suggesting adaptive trade-off during host adaptation. Four late-stage isolates showed enhanced pyoverdine production and improved growth under iron limitation, indicating progressive airway adaptation to the iron-restricted airway niche.IMPORTANCEPseudomonas aeruginosa is a leading cause of chronic airway infection and poses a severe clinical threat when it develops carbapenem resistance. While the adaptive evolution of P. aeruginosa has been well characterized in cystic fibrosis, its evolutionary patterns during chronic infection in patients with chronic obstructive pulmonary disease (COPD) remain poorly understood. Here, we analyzed six imipenem-resistant P. aeruginosa isolates sequentially recovered from a single COPD patient. Integrated genomic and phenotypic analyses demonstrated that the high-risk clone ST274 diversified extensively within the host, with genetic variations strongly associated with enhanced airway persistence. These findings advance our understanding of how carbapenem-resistant P. aeruginosa adapts during chronic COPD infection and provide new insights into resistance evolution and adaptive strategies that may inform the control of drug-resistant chronic respiratory infections.
    Keywords:  P. aeruginosa; adaptive; imipenem-resistant; mucoid; non-mucoid
    DOI:  https://doi.org/10.1128/spectrum.01358-26