Adv Sci (Weinh). 2026 Jan 04.
e17423
Yuling Cai,
Tongtong Li,
Qian Fang,
Ziyou Bao,
Feng Kong,
Huitao Qi,
Hanzhen Li,
Mingyu Zhang,
Wei Wang,
Yongxia Guan,
Wenbo Liu,
Xiangfeng Chen,
Zi-Jiang Chen,
Xiaohua Jiang,
Xin Wang,
Hongbin Liu.
Eukaryotic translation initiation factor 5A (eIF5A) facilitates protein synthesis and impacts diverse biological processes, yet its role in transcriptional regulation is poorly understood. Here eIF5A highly expressed in diverse spermatogenic cell types are found. Conditional knockout of Eif5a (SKO) causes complete infertility in male mice due to round spermatid arrest. Interestingly, eIF5A deletion severely compromises chromocenter integrity in round spermatids. Proteomic profiling reveals widespread dysregulation in eIF5A-deficient round spermatids, downregulated proteins are enriched for chromatin-associated functions, likely contributing to chromocenter dysfunction. Notably, ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) analysis shows increased chromatin accessibility upon eIF5A depletion, accompanied by transcriptional dysregulation of genes critical for acrosome and manchette formation. This data underscore that eIF5A not only regulates the translation of chromatin-organizing proteins required for chromocenter stability but also influences transcriptional regulation by modulating chromatin landscape. These findings illuminate a previously uncharacterized and germ cell-specific pathway coupling translational control and transcriptional regulation via chromatin reorganization.
Keywords: chromatin accessibility; eIF5A; male infertility; spermatogenesis; translational‐transcriptional coupling