bims-imicid Biomed News
on Immunometabolism of infection, cancer and immune-mediated disease
Issue of 2026–05–31
thirty-six papers selected by
Dylan Gerard Ryan, Trinity College Dublin



  1. J Am Heart Assoc. 2026 May 25. e047847
      Atherosclerosis is a chronic inflammatory disease driven by metabolic disorders, and macrophages play a central role in their occurrence and development. Macrophages are not static; their functional polarization and fate decisions are highly regulated by metabolic signals from the microenvironment, a process known as metabolic reprogramming. This review systematically reviews the latest progress in the metabolic reprogramming of glucose, lipids, and mitochondria in atherosclerosis, focusing on how key metabolites such as glycolysis, pentose phosphate pathway, cholesterol/sphingolipid metabolism, gut microbiota derivatives, and oxaloacetate dynamically regulate the inflammatory phenotype, foam cell formation, and immune response of macrophages. This review also delves into new concepts such as trained immunity and analyzes the therapeutic potential of targeting these metabolic pathways, aiming to comprehensively reveal the core role of metabolic-immune cross-talk in atherosclerosis and provide a theoretical basis for developing new strategies for diagnosing and treating atherosclerosis based on macrophage metabolic reprogramming.
    Keywords:  atherosclerosis; gut microbiota; itaconate; macrophage metabolic reprogramming; trained immunity
    DOI:  https://doi.org/10.1161/JAHA.125.047847
  2. Redox Biol. 2026 May 21. pii: S2213-2317(26)00228-4. [Epub ahead of print]94 104230
      Obesity is a well-established risk factor for increased severity and mortality in acute pancreatitis. However, the mechanisms by which obesity alters pancreatic immune regulation and favors the progression of acute pancreatitis are not elucidated yet. Here, we identify a neutrophil-driven immune-metabolic pathway that controls ferroptosis during pancreatic inflammation. We show that infiltrating myeloid cells represent the principal source of the immunometabolite itaconate during acute pancreatitis. Through paracrine transfer via the SLC13A3 transporter, myeloid-derived itaconate protects pancreatic acinar cells from ferroptosis by sustaining NRF2-dependent antioxidant responses. Obesity disrupts this protective axis by suppressing ACOD1 expression in infiltrating neutrophils. Proteomic profiling of pancreatic neutrophils from obese mice confirmed reduced ACOD1 abundance and decreased expression of enzymes linked to the tricarboxylic acid cycle and pyruvate metabolism. This metabolic reprogramming limits itaconate production and weakens NRF2-driven redox defenses, leading to downregulation of the xCT-GPX4 ferroptosis-protective pathway and increased lipid peroxidation in the pancreas of obese mice with pancreatitis. Pharmacological restoration of itaconate signaling with the cell-permeable derivative 4-octyl itaconate reactivates NRF2 signaling, the xCT-GPX4 antioxidant axis, and the trans-sulfuration pathway, mitigating pancreatic injury. Together, these findings identify neutrophil-derived itaconate as a key modulator of ferroptosis susceptibility and reveal immune cell metabolism as a critical determinant of obesity-associated severity in acute pancreatitis.
    DOI:  https://doi.org/10.1016/j.redox.2026.104230
  3. Int J Biol Sci. 2026 ;22(10): 5228-5245
      Chimeric antigen receptor T (CAR-T) cell therapy has revolutionized the treatment of hematologic malignancies; however, its efficacy in solid tumors remains limited, partly due to T cell exhaustion during ex vivo manufacturing. Emerging evidence suggests that cholesterol metabolism plays a critical role in T cell differentiation and function, yet its impact during CAR-T cell production is poorly understood. We investigated the effects of cholesterol modulation during ex vivo CAR-T cell expansion by using low-dose fluvastatin (FL), a clinically approved HMG-CoA reductase inhibitor. We found that cholesterol accumulation during ex vivo expansion promotes CAR-T cell exhaustion. Low-dose FL reduces cholesterol to physiological levels, preserving a less-differentiated, memory-enriched phenotype and attenuating exhaustion, thereby enhancing CAR-T cell cytotoxicity and persistence without affecting viability. In multiple xenograft models, FL-primed CAR-T cells demonstrate superior in vivo expansion, persistence, and tumor control. Mechanistically, FL enhances ERK1/2 phosphorylation to remodel CAR-T cell metabolism from glycolysis to oxidative phosphorylation. Inhibiting ERK1/2 or ATP synthesis abrogates these benefits, indicating that ERK1/2-dependent mitochondrial metabolism is required for CAR-T cell functional improvements conferred by FL. These findings establish cholesterol metabolism as a tunable axis during CAR-T cell manufacturing and propose a clinically feasible, GMP-compatible strategy to enhance CAR-T cell fitness and therapeutic efficacy.
    Keywords:  CAR-T cells; cholesterol; low-dose FL; metabolic remodeling
    DOI:  https://doi.org/10.7150/ijbs.132207
  4. Cell Mol Immunol. 2026 May 28.
      Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is the leading cause of infectious disease-related death. As a major intracellular pathogen, Mtb can escape clearance by the immune system, but the underlying molecular mechanisms remain incompletely elucidated. Specific genomic regions of deletion (RD)-encoded proteins in virulent Mtb H37Rv have been implicated in modulating pathogenicity and immunity. Here, we report a novel RD15-encoding protein, Rv1977 (a mycobacterial cell wall protein with a size of 39 kDa, named MEM39), which facilitates Mtb survival in macrophages. The survival of the Mtb H37Rv MEM39-deficient strain is reduced in both macrophage and murine infection models. Furthermore, the mycobacterial MEM39 protein binds fructose-diphosphate aldolase A (ALDOA), a key enzyme of glycolysis, thereby impairing ALDOA enzyme activity, disrupting macrophage metabolite flux, and reducing lactate production. The MEM39-ALDOA interaction also suppresses lysosomal acidification; reduces NLRP3 inflammasome activation and the production of proinflammatory cytokines (TNF-α, IL-6 and IL-1β); and thereby promotes bacterial survival within macrophages. Disruption of the interaction between MEM39-ALDOA and a cell-penetrating synthetic peptide (VLARYASICQ) significantly suppressed Mtb survival by restoring lactate production, lysosome acidification and proinflammatory cytokine production in both macrophage and mouse infection models. These findings revealed that mycobacterial MEM39 negatively regulates host immune defense through reprogramming ALDOA-mediated glycolysis in macrophages, thereby forming a "mycobacterial MEM39 virulence factor-glycolysis metabolism-immunity" regulatory axis. Targeting MEM39 or the MEM39-ALDOA interaction interface holds promise as a new therapeutic strategy against tuberculosis.
    Keywords:  ALDOA; Glycolytic metabolism of macrophages; Mycobacterium tuberculosis; Phagosome acidification; Rv1977/MEM39; Virulence factor; bacterial intracellular survival
    DOI:  https://doi.org/10.1038/s41423-026-01431-w
  5. Mol Neurobiol. 2026 May 26. pii: 651. [Epub ahead of print]63(1):
      Chronic pain is traditionally framed as a consequence of neuroinflammation and maladaptive synaptic plasticity, with activated microglia releasing cytokines, chemokines, and growth factors that sensitize nociceptive circuits in the spinal dorsal horn. However, microglial activation is also accompanied by profound metabolic reprogramming-including a glycolytic shift, altered mitochondrial dynamics, and increased demand for biosynthetic intermediates-that has received comparatively little attention in pain neurobiology. Here, we propose that metabolic competition between activated microglia and neighboring neurons may constitute an underexplored mechanism contributing to persistent pain states. We argue that shifts in local energy allocation-particularly glucose, lactate, and nicotinamide adenine dinucleotide (NAD+) availability-could modulate neuronal excitability and sustain central sensitization even when classical inflammatory signaling is no longer dominant. Drawing on advances in immunometabolism, emerging single-cell/spatial metabolomics, and in vivo biosensor imaging, we integrate neuroimmunology with metabolic neurobiology to generate experimentally testable predictions. If validated, this framework could reposition cellular metabolism as a tractable therapeutic dimension for chronic pain management.
    Keywords:  Astrocyte–neuron lactate shuttle; Central sensitization; Chronic pain; Dorsal horn; Glycolysis; Immunometabolism; Metabolic reprogramming; Microglia; Mitochondrial dysfunction; Warburg effect
    DOI:  https://doi.org/10.1007/s12035-026-05952-3
  6. Virol J. 2026 May 24. pii: 131. [Epub ahead of print]23(1):
      Viruses strongly depend on the host cell for efficient replication and influenza A virus (IAV) amongst others also lead to remarkable changes of the host cell metabolism. The restriction of virus replication through suppression of glucose metabolism has already been described. In addition to glycolysis and glutaminolysis, viral replication also relies on the tricarboxylic acid (TCA) cycle. So far, the metabolic key intermediate of the TCA cycle, oxaloacetate (OAA), is described to enhance glycolysis and respiration flux rates under metabolic stress conditions. However, the mode of action of the metabolic fuel intermediate OAA in direct relation to influenza viral growth under strong glycolysis inhibition remains unclear. As the TCA cycle acts as a central metabolic hub linking all major metabolic pathways, the effects of OAA under glycolysis inhibition were examined in greater detail in this study. We aimed to get a better understanding of metabolic host-virus interactions and to analyze the effects of metabolic fueling intermediates on IAV replication under glycolysis inhibition. We inhibited glycolysis and supplemented IAV infected cells with the metabolic fueling intermediate OAA. Inhibition of glycolysis led to a statistically significant reduction of viral titers while OAA addition reversed the antiviral effects such as reduced viral protein accumulation, viral titers, and vRNA expression. In line with previous studies, we showed that mannose, which is closely connected to glycolysis, circumvents the virus restricting effects of glycolysis inhibition. Moreover, we demonstrated that supplementation of mannose or OAA led to a roughly comparable replication recovery under strong inhibition of glycolysis. Furthermore, mass spectrometry-based metabolomics data revealed a strong accumulation of pyruvate in OAA supplemented samples. Finally, comparing OAA and pyruvate rescuing capacities of IAV growth under glycolysis inhibition tended to show similar activities for both metabolites arguing that OAA mediated IAV rescue is achieved through its conversion to pyruvate. Summarizing, our data indicate that the TCA cycle intermediate OAA has virus supporting effects as it reversed the antiviral effects of glycolysis inhibition.
    DOI:  https://doi.org/10.1186/s12985-026-03201-6
  7. Front Immunol. 2026 ;17 1845654
      Allergic asthma is characterized by type 2 inflammation and overnight worsening of symptoms, yet dynamic fluxes in cellular metabolic profiles driving time-of-day variation remain poorly defined. Group 2 innate lymphoid cells (ILC2s) are central mediators of airway hyperreactivity. We identify melatonin as a previously unrecognized regulator of ILC2 metabolism and function. In murine models of allergic airway inflammation, melatonin reduced eosinophilia, type 2 cytokine production, and airway hyperreactivity without altering ILC2 abundance. Mechanistically, melatonin acted independently of canonical melatonin receptors and instead reprogrammed ILC2 metabolism toward pentose phosphate pathway activity, enhancing NADPH generation and NRF2-dependent glutathione accumulation. Metabolic profiling, loss-of-function approaches, and pharmacologic activation studies demonstrated that NRF2 is both necessary and sufficient to restrain ILC2 effector function. Importantly, primary human ILC2s exhibited conserved NRF2 activation, glutathione accumulation, and reduced type 2 cytokine production in response to melatonin, underscoring clinical relevance. Together, these findings identify the melatonin-NRF2-glutathione axis as a metabolic checkpoint regulating innate type 2 immunity and suggest that therapeutic targeting of redox metabolism may represent a strategy for modulating airway inflammation in allergic asthma.
    Keywords:  allergic asthma; immunometabolism; innate immunity; innate lymphoid Cells (ILC2); pentose phosphate pathway; redox homeostasis and signaling; respiratory immunology
    DOI:  https://doi.org/10.3389/fimmu.2026.1845654
  8. Anticancer Res. 2026 Jun;46(6): 2977-2984
      The immune system plays an essential role in protecting the host from malignant transformation through immune surveillance, a concept that was later expanded by the cancer immunoediting framework. While immune responses eliminate emerging tumors, they can also impose selective pressure that promotes tumor adaptation and immune escape. Among the mechanisms involved in this process, metabolic immune suppression has gained increasing attention, as it weakens immune function without causing immune cell death. In this context, the semi-essential amino acid L-arginine has emerged as an important metabolic factor regulating immune competence within the tumor microenvironment. The availability of arginine directly affects immune cell activation, proliferation, and effector function, acting as a metabolic checkpoint rather than only as a biosynthetic substrate. Tumors actively generate arginine-poor niches through the expression of arginine- metabolizing enzymes in tumor cells as well as in immunosuppressive myeloid and stromal populations. As a result, immune cells, particularly T lymphocytes, undergo a reversible state of functional paralysis characterized by preserved viability but impaired cell-cycle progression, reduced protein synthesis, and weakened effector responses. In contrast, tumor cells often tolerate or adapt to low arginine levels, leading to a metabolic imbalance that selectively suppresses antitumor immunity. In this review, we summarize current knowledge on arginine- dependent immune suppression, focusing on arginase- and nitric oxide synthase-mediated pathways, competition for arginine transport, and nutrient-sensing signaling through mTORC1 and GCN2. We also discuss the evolutionary conservation and reversibility of this mechanism and its interplay with other immunosuppressive metabolic pathways. Finally, we highlight therapeutic strategies targeting arginine metabolism, emphasizing the arginine system as a key regulator of tumor-induced immune suppression and a potential target for cancer immunotherapy.
    Keywords:  Arginine metabolism; T cell anergy; cancer immunoediting; immunometabolism; review; tumor microenvironment
    DOI:  https://doi.org/10.21873/anticanres.18174
  9. Genome Med. 2026 May 29. pii: 74. [Epub ahead of print]18(1):
       BACKGROUND: Interferons play a central role in antiviral defense, but their dysregulation contributes to inflammation and immune dysfunction in respiratory viral infections, including COVID-19. While interferon-stimulated genes (ISGs) are essential effectors of this response, their expression patterns in patients are heterogeneous and not always predictive of disease severity. The immunometabolic consequences of this heterogeneity remain poorly understood.
    METHODS: We analyzed hospitalized COVID-19 patients (n = 37) and uninfected controls (n = 31) using whole-blood transcriptomics, immune cell deconvolution, plasma proteomics, and standardized plasma metabolomics from a previously generated dataset within this cohort. Patients were stratified into low (LIS), moderate (MIS), and high (HIS) ISG score clusters. Plasma innate immune activation markers were measured by ELISA. Interferon-directed antibody reactivity was analyzed by multiplex bead-based assays measuring antigen reactivity. Functional immune responses were assessed via ex vivo stimulation of healthy donor immune cells with patient plasma, and correlations were performed between metabolites and immune activation markers.
    RESULTS: HIS patients exhibited increased inflammatory mediators and innate immune cell expansion compared with LIS and MIS groups. However, within the HIS group, severe cases displayed distinct metabolic and immune dysregulation. Specifically, severe HIS cases showed reductions in phospholipids, sphingolipids, and tricarboxylic acid cycle intermediates, suggestive of disrupted mitochondrial and lipid metabolism. Plasma from severe HIS patients tended to impair neutrophil and monocyte activation, indicating functional attenuation of innate immune activation within a shared high-ISG background. Correlation analysis revealed that branched-chain lipids, tryptophan-derived metabolites, and a branched-chain dicarboxylic acid were positively associated with immune activation markers. Although type-I interferon neutralization was detected in a subset of patients with IFN antigen reactivity, these samples did not fully account for the observed ISG heterogeneity or disease severity.
    CONCLUSIONS: High ISG expression in COVID-19 defines a transcriptional endotype associated with systemic inflammation and innate immune activation. However, severe cases within this group exhibit metabolic constraints and reduced innate immune responsiveness, supporting an immune-metabolic axis in which inflammatory mediators and altered lipid/energy metabolism intersect with innate immune function. These findings motivate future studies to determine whether interferon-associated immune-metabolic states during acute infection relate to persistent inflammation or post-acute sequelae in selected patient subsets.
    Keywords:  COVID-19; Immune-metabolic dysregulation; Interferon autoantibody; Interferon-stimulated genes (ISGs); Metabolic changes; Neutrophil activation; Type I interferons
    DOI:  https://doi.org/10.1186/s13073-026-01677-z
  10. Int J Mol Sci. 2026 May 21. pii: 4619. [Epub ahead of print]27(10):
      High lactate concentration is a hallmark of the tumor microenvironment (TME). Regulatory T cells (Tregs) exhibit unique metabolic adaptability to this lactate-rich environment, yet the underlying mechanisms remain incompletely understood. Here, we demonstrate that the monocarboxylate transporter MCT4 is upregulated in tumor-infiltrating Tregs and mediates direct lactate uptake. Using Treg-specific conditional knockout (cKO) mice, we show that MCT4 deficiency does not affect basal Treg development but abrogates lactate-induced Foxp3 stabilization and impairs Treg suppressive function. Mechanistically, MCT4-mediated lactate uptake promotes the lactylation of Foxp3 at lysine 277 (K277), which competitively inhibits its ubiquitination, thereby enhancing Foxp3 protein stability and nuclear localization. Nuclear Foxp3 subsequently interacts with IRF3 to promote IL-10 transcription and secretion. In the B16 melanoma model, MCT4-deficient Tregs display compromised stability and reduced tumor infiltration, leading to enhanced CD8+ T cell effector function and attenuated tumor growth. Collectively, our findings reveal that MCT4-mediated lactate uptake sustains Treg stability and function through Foxp3 lactylation, identifying MCT4 as a potential therapeutic target for modulating Treg activity in cancer.
    Keywords:  MCT4; lactate; lactylation; treg; tumor immunity
    DOI:  https://doi.org/10.3390/ijms27104619
  11. Front Immunol. 2026 ;17 1746724
       Background: Sepsis is a life-threatening condition with high mortality and limited therapeutic options. This study investigated the association between plasma cholesterol levels and sepsis survival and explored the mechanisms by which elevated cholesterol confers protection.
    Methods: We analyzed 2,787 sepsis patients from the MIMIC-IV database, comparing cholesterol levels between 28-day survivors and non-survivors and assessing mortality risk using multivariable Cox regression. To test causality, C57BL/6J mice were fed either a high-cholesterol diet (HCD) or a regular diet (RD) before cecal ligation and puncture (CLP).
    Results: Survivors had significantly higher cholesterol than non-survivors (median 135 vs. 126 mg/dL; p < 0.001). High cholesterol (≥133 mg/dL) was independently associated with reduced 28-day mortality (adjusted HR = 0.80; 95% CI: 0.67-0.95; p = 0.012). In mice, HCD elevated plasma cholesterol and improved survival (52.5% to 90%), through a mechanism that is not primarily explained by broad immune activation. Hepatic transcriptomics revealed metabolic reprogramming, including enhanced oxidative phosphorylation and antioxidant pathways, with suppression of endoplasmic reticulum proteostasis. Inhibition of mitochondrial respiration abolished the survival benefit.
    Conclusions: Elevated plasma cholesterol is associated with improved sepsis outcomes, likely through promoting hepatic metabolic reprogramming. Targeting hepatic bioenergetics is a potential therapeutic approach.
    Keywords:  cholesterol; high cholesterol diet (HCD); metabolic reprogramming; oxidative phosphorylation; sepsis
    DOI:  https://doi.org/10.3389/fimmu.2026.1746724
  12. Elife. 2026 May 26. pii: RP109206. [Epub ahead of print]14
      Metabolic dysfunction-associated steatotic liver disease (MASLD) progression is characterized by hepatic inflammation and cell death, yet the mechanisms underlying Kupffer cell (KC) loss remain poorly understood. Here, we sought to elucidate the metabolic basis of KC death during MASLD. Using metabolomics, immunostaining, and flow cytometry, we evaluated metabolic alterations and KC death throughout early MASLD progression. We found that KC death is an early hallmark of MASLD, exhibiting greater susceptibility and a spatial distribution consistent with KC zonation. Moreoever, KCs undergo progressive metabolic reprogramming toward enhanced glucose utilization during MASLD development, which is correlated with KC death. In combination with biochemical agonist, isotope tracing, and primary KC culture, we further demonstrated that augmented glycolytic metabolism directly drives KC death in vitro. Consistently, using Chi3l1-deficient mice, we further demonstrated that increased glucose utilization accelerates KC death in vivo. Together, these findings establish a causal link between glycolytic activation and KC loss during MASLD progression, highlighting glucose metabolic pathways as potential therapeutic targets to preserve KC homeostasis and mitigate MASLD.
    Keywords:  Kupffer cells; MASLD; cell death; glycolytic metabolism; medicine; metabolic reprogramming; mouse
    DOI:  https://doi.org/10.7554/eLife.109206
  13. mBio. 2026 May 29. e0084626
      Polyamines integrate metabolism with innate immunity; however, their antibacterial roles remain unresolved. Here, we show that spermine suppresses innate immune responses against Salmonella enterica serovar Typhimurium infection in macrophages. Exogenous spermine blunted pro-inflammatory responses and increased intracellular bacterial burden in vitro and in vivo. Pharmacologic depletion or back-conversion of endogenous polyamines enhanced pro-inflammatory responses, revealing an intrinsic brake. Mechanistically, spermine promoted B-to-Z conformational switching of bacterial genomic DNA, which attenuated cGAS-STING activation; chloroquine or cerium chloride modulations validated DNA topology control. Genetic and pharmacologic dissection indicated that either TLR4 or cGAS-STING is sufficient for spermine's suppression, whereas their combined blockade abolished it. These findings highlight coordinated immune evasion at the metabolism-DNA interface, offering therapeutic targets for infection control.
    IMPORTANCE: In our research, we set out to understand how a small, naturally occurring molecule called spermine shapes the first line of defense against Salmonella, a common cause of foodborne illness. We discovered that when Salmonella infects our immune cells, it cleverly causes the levels of spermine to drop. When we experimentally added spermine back, we found that it hampered the immune response, allowing the bacteria to thrive. Delving deeper, we uncovered a two-pronged strategy used by spermine. It not only dampens a primary alarm pathway that detects bacteria on the cell surface but also employs a more subtle tactic inside the cell. Spermine changes the physical shape of the bacterial DNA, twisting it into a form that our internal surveillance systems, like the cGAS-STING pathway, cannot easily recognize. This disguise allows the bacteria to go undetected. Our findings reveal a fascinating mechanism of immune evasion and suggest that controlling spermine levels could be a promising therapeutic strategy for either boosting our defenses against Salmonella infections or calming inflammatory diseases.
    Keywords:  DNA conformational switching; Salmonella Typhimurium; cGAS-STING pathway; innate immune response; macrophages; polyamines; spermine
    DOI:  https://doi.org/10.1128/mbio.00846-26
  14. Front Immunol. 2026 ;17 1822668
      Chimeric antigen receptor (CAR)-T cell therapy has achieved remarkable success in hematological malignancies, yet its efficacy in solid tumors is severely limited by the metabolically hostile tumor microenvironment (TME). Within this landscape, CAR-T cells undergo rapid functional exhaustion driven by mitochondrial dysfunction and metabolic insufficiency. This mini-review synthesizes emerging mitochondrial engineering strategies designed to restore metabolic fitness and persistence. We first examine the newly identified metabolic-epigenetic axis, where the pathological mitochondrial translocation of P4HA1 and the concomitant accumulation of oncometabolite succinate lock T cells in an exhausted state, and discuss how targeting this pathway restores progenitor subsets. Furthermore, we explore genetic reprogramming approaches, including "Envirotune" platforms that couple hypoxia-sensing elements (HRE) with enhanced glutamine transport (SLC38A2), and CRISPR-identified targets such as RHOG and FAS that prevent fratricide and apoptosis to preserve effector pools. Finally, we highlight the frontier of organelle medicine, focusing on intercellular mitochondrial transfer via tunneling nanotubes (TNTs) mediated by Talin-2, and emerging computational strategies to detect mitochondrial hijacking risk. By integrating these metabolic interventions, next-generation CAR-T cells can be engineered to overcome the TME's metabolic barriers, transforming them from transient effectors into long-lived, highly effective therapeutic agents.
    Keywords:  CAR-T cell therapy; T cell exhaustion; immunometabolism; metabolic reprogramming; metabolic-epigenetic axis; mitochondrial engineering; solid tumors; tumor microenvironment
    DOI:  https://doi.org/10.3389/fimmu.2026.1822668
  15. Cell Rep. 2026 May 28. pii: S2211-1247(26)00525-5. [Epub ahead of print]45(6): 117447
      Alveolar macrophages (AMs) reside in the oxygen-rich alveoli, where hypoxia-inducible transcription factor (HIF) subunits are targeted for degradation by the Von Hippel-Lindau protein (pVHL). We previously showed that Vhl-deficient AMs are immature and functionally impaired. Here, we define isoform-specific roles of HIF-1α and HIF-2α in the regulation of AM maturation and function. Expression of either isoform alone is sufficient to intrinsically, and differentially, impair AM terminal maturation and self-renewal, with complete rescue observed only when both HIF-1α and HIF-2α are deleted in Vhl-deficient AMs. HIF-1α drives glycolytic reprogramming in AMs, while HIF-2α disrupts fatty acid oxidation and surfactant clearance. Consequently, HIF-2α stabilization limits the capacity of AMs to resolve surfactant excess in a mouse model of pulmonary alveolar proteinosis, indicating HIF-2α as a potential therapeutic target. Overall, HIF inactivation ensures optimal AM maturation and metabolic adaptation to the high-oxygen alveolar niche, revealing non-redundant functional specificities of each HIF-α isoform.
    Keywords:  Alveolar Macrophage; CP: Immunology; CP: Metabolism; HIF; glycolysis; mitochondrial respiration; self-renewal; surfactant oxidation
    DOI:  https://doi.org/10.1016/j.celrep.2026.117447
  16. Methods Cell Biol. 2026 ;pii: S0091-679X(26)00022-1. [Epub ahead of print]207 19-51
      Mitochondria play a central role in cellular metabolism, ATP production, and redox homeostasis, all of which are essential for sustaining T lymphocyte and CAR-T cell effector functions. Mitochondrial dysfunction has been linked to T cell and CAR-T cell exhaustion, reduced cell expansion capacity, and impaired tumor clearance. To investigate the contribution of mitochondria to T lymphocyte and CAR-T cell functionality, this protocol leverages advanced imaging analysis techniques to assess different parameters related to mitochondrial dynamics, structure and function, which may serve as indicators of both metabolic activity and the exhausted state of T cells. Specifically, the protocol focuses on measuring mitochondrial morphology, energization state, and mitochondrial translocation in the three-dimensional space. The image-based methodologies described in this protocol will contribute to a deeper understanding of mitochondrial regulation during T-cell and CAR-T cell responses. This knowledge will facilitate the identification of key metabolic vulnerabilities and support the refinement of therapeutic strategies to enhance T lymphocyte cytotoxic potential, ultimately improving clinical outcomes in T cell-based adoptive cancer immunotherapy.
    Keywords:  Actin cytoskeleton; Chimeric antigen receptor; Immune synapse; Mitochondria; T lymphocytes
    DOI:  https://doi.org/10.1016/bs.mcb.2026.01.022
  17. Front Immunol. 2026 ;17 1735359
      Patients with diabetic sepsis exhibit a paradoxical state characterised by persistently elevated inflammatory cytokines and severely impaired antigen presentation, with substantially elevated mortality rates compared to non-diabetic patients. This review assesses the strength of evidence for lactate-epigenetic-immune dysfunction. Immune cells from diabetic patients exhibit basal glycolytic activity 2-3 times higher than healthy controls. Blood lactate levels rise markedly during sepsis, exceeding 10 mmol/L in critically ill patients-50-80% higher than non-diabetics. Hyperlactataemia states have been associated with activation of GPR81 receptors and induction of lactylation at histone H3K18. This modification selectively activates inflammatory genes while suppressing antigen-presentation pathways, thereby providing a molecular basis for the paradoxical coexistence of inflammation and immunosuppression observed clinically. Preliminary clinical studies (n = 48) demonstrate a correlation between H3K18la levels and disease severity (r = 0.63). In addition, lactate clearance of <30% within 6 hours is associated with poor prognosis. Current therapeutic evidence remains limited: dichloroacetic acid reduces serum lactate by 20-30% but shows no proven survival benefit; GPR81 modulators remain in development; GM-CSF may increase HLA-DR expression but demonstrates inconsistent effects on infection and mortality. This review identifies three potential therapeutic targets: metabolic regulation to reduce lactate production, intervention in the GPR81-H3K18la signalling axis, and personalised therapy based on immune phenotypes. However, these strategies require validation through high-quality clinical trials.
    Keywords:  GPR81 receptor; H3K18la; epigenetic reprogramming; histone lactylation; lactate metabolism
    DOI:  https://doi.org/10.3389/fimmu.2026.1735359
  18. Mol Immunol. 2026 May 26. pii: S0161-5890(26)00101-X. [Epub ahead of print]195 209-220
       BACKGROUND: Intestinal inflammation has emerged as a significant global concern in poultry farming, particularly due to its strong association with macrophage imbalance. Research has identified macrophages as critical target cells for butyrate, which collaboratively maintains intestinal immune homeostasis. This study delves into the regulatory effects of butyrate on macrophages to ameliorate enteritis in broiler chickens.
    RESULTS: Our findings indicate that broilers treated with dextran sulfate sodium (DSS) exhibit reduced body weight and compromised intestinal mucosal integrity, characterized by increased intestinal permeability, elevated histological scores, diminished goblet cell numbers, reduced secretion of MUC2 protein, and decreased expression of tight junction proteins, alongside heightened oxidative stress and apoptotic responses. Notably, DSS treatment induces the polarization of M0 macrophages towards the pro-inflammatory M1 phenotype, thereby exacerbating intestinal inflammation. Conversely, butyrate supplementation facilitates the polarization of macrophages towards the anti-inflammatory M2 phenotype by inhibiting M1 polarization, thereby mitigating intestinal inflammation and promoting the restoration of the intestinal mucosal barrier. Further in vitro experiments demonstrated that butyrate regulates macrophage polarization through activation of the HDAC3/AMPK/PPARγ/Hippo/GLUT1 signaling pathway, ultimately exerting a beneficial effect.
    CONCLUSION: Butyrate has been shown to facilitate M2 macrophage polarization by modulating cellular metabolic pathways, thereby ameliorating intestinal inflammation. This study underscores the clinical significance of immunometabolism in intestinal diseases and identifies potential therapeutic targets, serving as a valuable reference for the clinical management of enteritis in broilers.
    Keywords:  Butyrate; Dextran sulfate sodium; Enterotoxicity; Macrophages
    DOI:  https://doi.org/10.1016/j.molimm.2026.04.017
  19. bioRxiv. 2026 May 15. pii: 2026.05.14.725218. [Epub ahead of print]
      Macrophages are crucial for host defense against the pathogen. However, pathogens such as Mycobacterium tuberculosis (Mtb) have evolved mechanisms to alter macrophage physiology and exploit these cells as their primary niche. Mtb -infected macrophages upregulate several metabolic pathways including glutamine metabolism. We previously showed that inhibiting glutamine metabolism with the pleiotropic glutamine metabolism antagonist prodrug JHU083 has dual antibacterial and immunomodulatory effects in a mouse model of tuberculosis. In the present study, using single-cell RNA sequencing and LS-MS/MS metabolomics, we showed that JHU083-mediated glutamine metabolism inhibition increased the population of interstitial macrophages in Mtb -infected lungs. JHU083 treatment also increased inflammatory signatures while lowering immunosuppressive markers on these macrophages. Metabolically, these macrophages exhibited marked depletion of complex lipids, accumulation of free fatty acids, and increased expression of transcripts associated with the β-oxidation pathway. Additionally, JHU083-treatment also improved phagocytic activity of macrophages, as measured by using fluorescent E. coli as a bait. In conclusion, JHU083-mediated glutamine metabolism inhibition metabolically reprograms macrophages, increasing both their lipid utilization as well as phagocytic activity, potentially driving their antimycobacterial activity that we had observed earlier.
    DOI:  https://doi.org/10.64898/2026.05.14.725218
  20. FASEB J. 2026 May 31. 40(10): e71958
      Macrophage foaming, characterized by uncontrolled uptake of oxidized LDL (ox-LDL) by macrophages, critically drives atherosclerosis (AS) progression. Although NK cells are found to be atherogenic, their direct impact on macrophage foaming remains unknown. Here we examined the role of NK cells in macrophage foaming and found that NK cells exacerbated macrophage cholesterol accumulation both in the presence/absence of ox-LDL. Under ox-LDL exposure, NK cells promoted cholesterol accumulation via increasing cholesterol influx related gene CD36, and significantly reducing the expressions of cholesterol efflux associated receptors ABCA1 and ABCG1 in the macrophages. When without ox-LDL, NK cells accelerated cholesterol synthesis via the SREBP-2-LDLR/HMGCR pathway, and inhibited cholesterol efflux via the LXR-α-ABCA1/ABCG1 pathway of macrophages. These factors eventually led to the accumulation of cholesterol in macrophages, resulting in the formation of macrophage foam cells. Further, for the first time, we revealed the TIGIT/CD155 signaling pathway as a critical regulatory mechanism for macrophage foam cell formation. Specifically, the downregulation of TIGIT in highly active NK cells altered its interaction with CD155, which influenced macrophage cholesterol metabolism via CD155 and ultimately promoted foam cell formation. Furthermore, direct blockade of CD155 on macrophages exacerbated cholesterol accumulation, thereby establishing CD155 as an important regulator in macrophage-derived foam cell formation. These findings not only confirm NK cells as important drivers of macrophage foam cell formation but also highlight CD155 as a potential therapeutic target for atherosclerosis.
    Keywords:  NK cells; TIGIT/CD155; atherosclerosis; cholesterol metabolism; macrophage foaming
    DOI:  https://doi.org/10.1096/fj.202601178R
  21. Int J Biol Sci. 2026 ;22(10): 5142-5160
      Ulcerative colitis (UC) is characterized by chronic intestinal inflammation accompanied by epithelial barrier dysfunction and profound metabolic stress; however, how metabolic cues are integrated to determine epithelial cell fate remains incompletely understood. Here, we identify a context-dependent METTL1-m7G-SLC7A11 regulatory axis that links metabolic stress to intestinal epithelial outcomes during UC progression. By integrating analyses of human UC tissues, DSS-induced acute and chronic colitis mouse models, and mechanistic in vitro experiments, we demonstrate that METTL1 enhances N7-methylguanosine (m7G) modification of SLC7A11 mRNA, thereby stabilizing the transcript and sustaining SLC7A11 expression in inflammatory settings. Functionally, SLC7A11 exhibits glucose-dependent dual effects. Under glucose-replete conditions, SLC7A11 supports cystine uptake, glutathione synthesis, and redox homeostasis, protecting epithelial integrity and limiting inflammation. In contrast, under glucose deprivation-a characteristic feature of inflamed UC mucosa-persistent SLC7A11 activation induces disulfide stress, cytoskeletal collapse, and disulfidptosis-associated epithelial injury. In vivo, inhibition of the METTL1/m7G/SLC7A11 axis exacerbates chronic DSS-induced colitis but alleviates acute DSS-induced colitis, revealing a switch from adaptive to maladaptive signaling with escalating metabolic stress. Collectively, these findings establish the METTL1-m7G-SLC7A11 axis as a metabolic rheostat that integrates inflammatory cues and nutrient availability to determine epithelial cell fate in UC, highlighting the importance of stage- and context-specific therapeutic strategies.
    Keywords:  Disulfidptosis; METTL1; Metabolic stress.; N7-methylguanosine (m7G); SLC7A11; Ulcerative colitis
    DOI:  https://doi.org/10.7150/ijbs.133562
  22. Biomaterials. 2026 May 22. pii: S0142-9612(26)00358-3. [Epub ahead of print]334 124334
      Periodontitis involves chronic inflammation with dysfunctional macrophage metabolism. To address this, we uniquely combined the biological properties of silk fibroin with a desolvation method and biomineralization principles, developing a multifunctional nanocomposite termed SF-HA@TA-Mn. It was engineered to function as a nanomodulator of intracellular metabolism. This system utilizes TA for mitochondrial targeting and Mn ions for enhanced antioxidant activity, enabling precise scavenging of mtROS. By restoring mitochondrial homeostasis, the nanomodulator effectively reprograms macrophage metabolism, shifting polarization from a pro-inflammatory M1 to a reparative M2 phenotype, thereby remodeling the local immune microenvironment. Integrated multi-omics analyses revealed the nanomodulator primarily reprograms arginine metabolism by downregulating the JAK2-STAT1-ASS1 axis, while reversing inflammatory alterations in glutamine-fueled TCA cycle and purine metabolism. Additionally, it inhibits inflammatory damage in hPDLSCs and uses the osteoconductive property of SF-HA to synergistically couple immunomodulation with osteogenic differentiation. In vivo evaluation using a rat periodontitis model confirmed the dual efficacy of SF-HA@TA-Mn NPs, demonstrating significant anti-inflammatory effects and enhanced alveolar bone regeneration. By flipping the macrophage metabolic switch toward a reparative state, the nanomodulator effectively reverses the inflammatory oxidative stress cycle, actively promoting bone regeneration, and thereby offers a promising integrated therapeutic platform for periodontitis management.
    Keywords:  Metabolic reprogramming; Nanomodulator; Periodontal bone regeneration; Silk fibroin nanoparticles
    DOI:  https://doi.org/10.1016/j.biomaterials.2026.124334
  23. Front Immunol. 2026 ;17 1808488
      Osteosarcopenic obesity (OSO)-the co-occurrence of osteoporosis/osteopenia, sarcopenia, and excess adiposity-is increasingly recognized in ageing populations and is strongly linked to frailty, fractures, disability, and cardiometabolic complications. However, heterogeneous operational definitions and population-specific cut-offs complicate risk stratification and mechanistic inference. Here, we propose a systems immunometabolic framework to explain coordinated deterioration of adipose tissue, skeletal muscle, and bone, focusing on chronic low-grade inflammation, trained immunity (innate immune memory), and senescence-associated signaling. Dysfunctional visceral adipose tissue emerges as an immune-active endocrine organ that sustains low-grade systemic inflammation through release of cytokines, adipokines, lipotoxic mediators, and damage-associated molecular patterns. A key mechanism potentially underpinning inflammatory persistence is trained immunity-epigenetic and metabolic reprogramming of innate immune cells and their progenitors-which establishes maladaptive inflammatory memory and amplifies inter-organ immune crosstalk. In skeletal muscle, this pro-inflammatory milieu promotes catabolic signaling and anabolic resistance, including NF-κB activation and mTOR pathway dysregulation, thereby driving impaired proteostasis, fibrosis, and fatty infiltration. In bone, inflammatory and senescence-associated signals converge on osteoclastogenic pathways and disrupt the receptor activator of nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) axis, leading to uncoupled bone remodeling and net bone loss. Collectively, we argue that OSO can be conceptualized as a fat-initiated, system-level immunometabolic remodeling process across the adipose-muscle-bone axis. This framework supports stratified, multimodal interventions combining lifestyle modification with mechanism-based anti-inflammatory and anti-resorptive therapies, while immuno-epigenetic and senescence-targeted approaches warrant further study. Notably, OSO-specific longitudinal and interventional evidence integrating immune phenotyping and multi-omics remains limited and is needed to test causality and validate actionable biomarkers and targets.
    Keywords:  Adipose-muscle-bone crosstalk; cellular senescence; chronic inflammation; immunometabolism; osteosarcopenic obesity; systems immunology; trained immunity
    DOI:  https://doi.org/10.3389/fimmu.2026.1808488
  24. Front Immunol. 2026 ;17 1808107
       Background: Cervical adenocarcinoma is an increasingly common and aggressive subtype of cervical cancer with marked biological heterogeneity. Accumulating evidence suggests that HPV-positive and HPV-negative adenocarcinomas exhibit distinct immune microenvironments, but the underlying mechanisms remain unclear.
    Methods: Publicly available single-cell RNA sequencing datasets of cervical adenocarcinoma and normal cervical tissues were systematically analyzed using integrated bioinformatic approaches, including cell clustering, copy number variation inference, metabolic pathway analysis, and cell-cell communication modeling. Key findings were validated through in vitro experiments using cervical cancer cell lines, macrophage polarization assays, metabolic measurements, ELISA, immunofluorescence, and CD8+ T cell functional analyses.
    Results: Single-cell analysis revealed profound differences in cellular composition and immune states between HPV-negative and HPV-positive adenocarcinomas. HPV-negative tumors exhibited increased immune infiltration but were enriched for exhausted CD8+ T cells and immunosuppressive SPP1+ macrophages. Malignant epithelial cells from HPV-negative adenocarcinoma displayed distinct metabolic reprogramming characterized by activation of cholesterol biosynthesis pathways, elevated DHCR7 expression, and accumulation of the oxysterol 27-hydroxycholesterol (27-HC). Functionally, 27-HC induced macrophage polarization toward an immunosuppressive phenotype and promoted SPP1 secretion. Macrophage-derived SPP1, in turn, enhanced DHCR7 expression and 27-HC production in tumor cells via CD44, forming a positive feedback loop that reinforced immune suppression. Disruption of DHCR7 attenuated macrophage-mediated immunosuppression and alleviated CD8+ T cell exhaustion.
    Conclusions: This study identifies a DHCR7-27-HC-SPP1 metabolic-immune axis that drives immune escape in HPV-negative cervical adenocarcinoma, highlighting cholesterol metabolism as a potential therapeutic vulnerability.
    Keywords:  DHCR7–27-HC–SPP1 axis; HPV-negative; cervical adenocarcinoma; cholesterol metabolism; immune microenvironment
    DOI:  https://doi.org/10.3389/fimmu.2026.1808107
  25. Blood. 2026 May 26. pii: blood.2025032136. [Epub ahead of print]
      Understanding how metabolism governs human hematopoietic stem cells (HSCs) function is essential for advancing regenerative therapies, yet direct metabolic profiling of human HSCs has been limited by their extreme scarcity and the technical limitations of conventional methods. Here, we apply a low-input mass spectrometry-based metabolomics platform, optimized for rare cell populations, to generate metabolic profiles of 13 immunophenotypically defined hematopoietic cell types from adult human bone marrow. Using as few as ~10,000 cells per sample, we detect over 80 metabolites and uncover both conserved metabolic programs in primitive hematopoietic stem and progenitor cells (HSPCs) and lineage-specific metabolic specializations. Notably, we identify L-carnitine-driven fatty acid oxidation (FAO) as a key metabolic feature supporting HSPC function. Mechanistically, L-carnitine activates the PPARA-TFEB signalling axis, promoting mitochondrial metabolism and autophagy to preserve regenerative capacity. Functional assays in primary CD34+ HSPCs derived from healthy donors or patients with aplastic anemia confirm that L-carnitine supplementation improves stem cell function ex vivo and in vivo. Together, this work provides a foundation for human hematopoietic metabolism and reveals a targetable metabolic circuit governing HSPC regenerative fitness with therapeutic potential for improving stem cell-based interventions.
    DOI:  https://doi.org/10.1182/blood.2025032136
  26. J Proteome Res. 2026 May 28.
      Macrophages are central players of innate immunity with diverse functions and involvement in numerous diseases, making it essential to understand their metabolic regulation. Here, we provide a comprehensive analysis of lipid metabolic dynamics during human monocyte-to-macrophage differentiation. Primary blood monocytes were differentiated for 5 days with either M-CSF or GM-CSF. Lipidomic profiling using the differential mobility spectrometry-shotgun lipidomics assistant (DMS-SLA) platform reliably quantified ∼400 lipids across 16 classes, while spectral flow cytometry was used to assess metabolic enzyme expression. Differentiation was marked by remodeling toward membrane lipids, accompanied by shorter acyl chains and reduced unsaturation in triglyceride (TG) and phosphatidylcholine (PC) species. PC species incorporated a more diverse range of pro- and anti-inflammatory precursors, whereas TG species mainly incorporated fatty acid FA 22:6. Metabolic enzyme expression showed dynamic, marker-specific changes over time, with G6PD and SDHA upregulation indicating enhanced pentose phosphate and oxidative phosphorylation pathways. M-CSF MDMs exhibited higher GLUT1 and CD36 expression and greater FA 22:6 incorporation within TGs. Together, these findings reveal extensive lipidome remodeling that underlies macrophage differentiation and distinct functional states.
    Keywords:  GM-CSF; M-CSF; lipidomic; metabolism; monocyte-derived macrophages
    DOI:  https://doi.org/10.1021/acs.jproteome.5c01060
  27. Nat Commun. 2026 May 27.
      Systemic inflammation is a hallmark of viral infection, but the upstream signals that initiate it remain poorly defined. Here we show that extracellular mitochondria act as inflammatory mediators during infection by vesicular stomatitis virus, influenza A virus, rabies virus, herpes simplex virus 1 and African swine fever virus (ASFV). In ASFV-infected primary porcine alveolar macrophages, the viral protein B66L promotes the capture of damaged mitochondria by autophagosomes while blocking their fusion with lysosomes, causing mitochondria to accumulate outside cells. Extracellular mitochondria are detected in the serum and bronchoalveolar lavage fluid of mice expressing B66L and in the serum of ASFV-infected pigs. Purified extracellular mitochondria trigger inflammatory cytokine production through cGAS-STING signalling and contribute to lung injury in mice. These findings identify virus-associated extracellular mitochondrial release as a pro-inflammatory mechanism during infection.
    DOI:  https://doi.org/10.1038/s41467-026-73537-8
  28. J Transl Med. 2026 May 26.
       BACKGROUND: Radiation-induced brain injury (RIBI) is a serious complication that occurs after cranial radiotherapy. The main manifestations are delayed radiation effects characterized by neuroinflammation and damage to neural stem cell populations. Microglia, the resident immune cells of the central nervous system (CNS), have become key mediators in the pathological process of RIBI. This review aims to systematically elucidate how metabolic reprogramming of lactate and lipid pathways in microglia contributes to chronic neuroinflammation and cognitive impairment following RIBI, and to evaluate the therapeutic potential of targeting these metabolic pathways.
    MAIN BODY: Ionizing radiation (IR) triggers intense activation of microglia, which initiates and maintains a chronic neuroinflammatory state characterized by the release of cytotoxic mediators and changes in phagocytic function. Changes in lactate and lipid metabolism within microglia are crucial in their response to neuroinflammation and neurodegeneration. Activated microglia typically change their metabolism from oxidative phosphorylation (OXPHOS), which uses oxygen to generate energy, to a process called aerobic glycolysis, which leads to increased lactate production. This metabolic shift, combined with the role of lactate as a signaling molecule and a substrate for epigenetic modifications (lactylation), can significantly influence the inflammatory outcome. Additionally, dysregulation of lipid metabolism, such as accumulation of lipid droplets (LDs), represents a pro-inflammatory, dysfunctional state known as lipid droplet accumulation-type microglia (LDAM), and is associated with impaired phagocytosis and persistent inflammation. This article summarizes the pathological mechanisms of RIBI, with a focus on the complex roles of lactate and lipid metabolism in microglia. It explores how radiation induces microglial activation and metabolic transformation. The article also discusses the dual role of lactate, the effects of lipid dysregulation, and potential interactions between metabolic pathways. Finally, it highlights how these factors commonly relate to impaired inflammatory responses and disruptions in neural repair processes, such as neurogenesis and oligodendrocyte generation.
    CONCLUSIONS: By studying how changes in microglial metabolism lead to neuronal dysfunction and cognitive decline in RIBI, this review provides a new perspective for regulating microglial metabolic pathways to alleviate radiation-induced cognitive impairment.
    Keywords:  Cognitive impairment; Lactate metabolism; Lipid metabolism; Metabolic reprogramming; Microglia; Neuroinflammation; Radiation-induced brain injury
    DOI:  https://doi.org/10.1186/s12967-026-08309-5
  29. EBioMedicine. 2026 May 28. pii: S2352-3964(26)00196-9. [Epub ahead of print]128 106314
       BACKGROUND: Pyruvate kinase M2 (PKM2) is an enzyme with moonlighting activities that controls murine T cell pro-inflammatory potential in a mouse model of multiple sclerosis (MS). However, no study analysed in detail the expression of PKM2 in human T cell subsets, and whether targeting PKM2 may limit the inflammatory potential of T cells from patients with MS is unknown.
    METHODS: In this observational, case control study we evaluated the expression of PKM2 in circulating T cells of healthy control individuals (HCs) and patients with MS, as well as its isomerisation in peripheral blood mononuclear cells (PBMCs) from HCs and patients. In parallel, we analysed the impact of targeting PKM2 on the inflammatory profile of T cells from HCs and individuals with MS.
    FINDINGS: Circulating effector and memory T cells express higher PKM2 levels compared to naïve T cells, and PKM2 expression in such T cell subsets may correlate with age and disease duration in individuals with MS. Among effector/memory T cells, the Th17/Tc17 subsets display the highest PKM2 expression. Additionally, we observed a preferential inhibition of interferon-gamma (IFN-γ), interleukin-5 (IL-5), IL-13, and IL-17 production by MS T cells upon PKM2 pharmacological targeting. Finally, we found that PBMCs from patients with MS have a higher percentage of PKM2 monomer compared to PBMCs from HCs, supporting heightened PKM2 moonlighting activity.
    INTERPRETATION: Our data suggest that PKM2 may represent a therapeutic target to limit T cell-driven inflammation in MS and potentially other human autoimmune diseases.
    FUNDING: Austrian Multiple Sclerosis Research Society, Kulturamt der Stadt Graz, MEFOGraz and Worldwide Cancer Research.
    Keywords:  Immunometabolism; Multiple sclerosis; PKM2; T cells
    DOI:  https://doi.org/10.1016/j.ebiom.2026.106314
  30. Front Immunol. 2026 ;17 1820993
      Sepsis is characterized by a progressive collapse of immune signal transduction, in which post-translational modifications (PTMs) act as critical execution layers that help shape the amplitude, duration, and reversibility of immune responses. Although often framed as a transition from cytokine storm to immune paralysis, the molecular logic governing this shift remains poorly defined. Growing evidence suggests that immune dysfunction in sepsis arises not from simple signal attenuation but from a loss of signaling competence, driven by coordinated changes in PTM networks, cellular metabolism, and chromatin structure. Here, we propose a metabolic-PTM temporal switch model as a conceptual framework in which immune signaling is rewired through context-dependent, PTM-associated configurations constrained by metabolic availability and chromatin accessibility. In early sepsis, permissive metabolic conditions and open chromatin may support fast, reversible PTMs-such as phosphorylation and scaffold-forming ubiquitination-that amplify innate immune signaling. As metabolic stress accumulates, a transition may occur in which ubiquitin linkage editing and increased deacetylation become more prominent and may contribute to the dismantling of signaling complexes and the restriction of transcriptional output. In late-stage sepsis, sustained metabolic exhaustion and chromatin condensation are associated with persistent PTMs, including histone lactylation, thereby potentially contributing to a low-plasticity immune state that becomes refractory to reactivation. Rather than implying a fixed temporal sequence, this framework is intended to describe representative PTM-associated patterns that may emerge across overlapping sepsis-related immune states. This framework may help explain why immune stimulation frequently fails in late sepsis: receptors and ligands may remain intact, yet signaling architecture and transcriptional competence can be substantially impaired. By identifying context-associated PTM patterns and signaling constraints, this model provides a conceptual basis for understanding context-dependent immune dysfunction and offers conceptual guidance for interpreting the variable outcomes of immune-targeted interventions in sepsis.
    Keywords:  epigenetic regulation; immunosuppression; metabolic signaling; post-translational modification; sepsis
    DOI:  https://doi.org/10.3389/fimmu.2026.1820993
  31. Mol Ther Oncol. 2026 Jun 18. 34(2): 201221
      Nicotinamide mononucleotide (NMN) supplementation has shown clinical benefits by regulating metabolic activities in the energy production process. Its protective effect and underlying immune regulatory mechanisms against tumor progression are still poorly understood. Here, we found that the high-dose NMN treatment could alter the level of several key NAD+ metabolic enzymes in human immune cells. Moreover, high-dose NMN treatment exhibited comparable antitumor efficacy as the PD-1 blockade in the murine mesothelioma challenge model. Subsequent immune profiling in both secondary lymphoid organ and tumor demonstrated that, rather than modulating T cell and NK cell responses, high-dose NMN treatment could reset tumor-associated macrophages toward the inflammatory M1-like phenotype compared with PD-1 blockade or non-treated subjects. These results provided a better understanding of NMN's regulatory effect on immune cells and suggested an alternative strategy of cancer immunotherapy.
    Keywords:  M1-like/M2-like phenotype; NAD+ salvage pathway; NMN; cancer; cytokine; enzyme; immune regulatory effect; macrophage; mesothelioma; nicotinamide mononucleotide
    DOI:  https://doi.org/10.1016/j.omton.2026.201221
  32. Front Immunol. 2026 ;17 1834919
      N6-methyladenosine (m6A) methylation is the most common intramolecular modification in eukaryotic mRNA; its dynamic regulation depends on "writers" (methyltransferases: METTL3/METTL14/WTAP/VIRMA), "erasers" (demethylases: FTO/ALKBH5), and "readers" (binding proteins: YTHDF/YTHDC/IGF2BP families), thereby regulating RNA splicing, nuclear export, translation, and degradation. In rheumatoid arthritis (RA), this epigenetic network is severely disrupted: abnormal expression of writers leads to post-transcriptional activation of pro-inflammatory genes, while an imbalance in erasers compromises the stability of mRNAs encoding key signaling molecules. Together, these factors promote abnormal differentiation of immune cells, invasive proliferation of fibroblast-like synovial cells, and cartilage erosion. At the same time, hypoxia, inflammatory cytokines, and metabolic stress present in the joint microenvironment of RA induce cellular metabolic reprogramming, characterized by a shift toward aerobic glycolysis (Warburg effect), a reorganization of lipid synthesis and oxidation pathways, and an increase in glutamine uptake and catabolism; these changes all contribute to accelerating disease progression. Recent data have revealed a foundational integration between m6A modification and metabolic reprogramming: m6A regulators directly reshape the metabolic network by targeting transcripts encoding the glycolysis-limiting enzyme (HK2), key molecules in lipid metabolism (FASN/CPT1), and amino acid transporters (SLC1A5), thereby coordinating immune inflammation and tissue destruction in RA. This review elucidates the regulatory role of m6A methylation in the metabolic reprogramming of RA and explains how writers, erasers, and readers influence disease progression by participating in glycolysis, lipid metabolism, and glutamine metabolism. By focusing on the central question of whether m6A modification is the root cause of metabolic reprogramming in the pathogenesis of RA, we have integrated existing data to define the "m6A-metabolism-immunity" regulatory axis and identified potential therapeutic strategies targeting this association.
    Keywords:  epigenetics; m6A methylation; metabolic reprogramming; rheumatoid arthritis; synergistic regulation
    DOI:  https://doi.org/10.3389/fimmu.2026.1834919
  33. Cell Mol Biol Lett. 2026 May 25.
      Acute lung injury (ALI) and its more severe form, acute respiratory distress syndrome (ARDS), are severe respiratory disorders characterized by a dysregulated and excessive inflammatory response within the pulmonary system. Recent studies have underscored the pivotal role of macrophage activation in driving inflammatory processes, with glycolytic reprogramming emerging as a critical regulator of macrophage function. In this study, we observed significantly elevated expression levels of G protein-coupled receptor 161 (GPR161) in peripheral circulating monocytes from patients with ARDS, with GPR161 expression positively correlating with disease severity. Utilizing genetically engineered mouse models, including global and macrophage-specific conditional knockout mice, we demonstrated that GPR161 deficiency attenuated pulmonary inflammatory damage in lipopolysaccharide-induced and sepsis-associated ALI mice. In vitro experiments further elucidated the essential role of GPR161 in macrophage activation and glycolytic reprogramming. Mechanistic investigations, integrating RNA sequencing with co-immunoprecipitation and surface plasmon resonance assays, identified complement component 5a receptor 1 (C5aR1) as a downstream target of GPR161 and showed that GPR161 promotes glycolytic reprogramming in macrophages by suppressing C5aR1 expression. Collectively, these findings demonstrate that GPR161 enhances macrophage activation and glycolytic reprogramming in ALI/ARDS through a C5aR1-dependent mechanism. These results establish macrophage GPR161 as a promising therapeutic target for the treatment of ALI/ARDS.
    Keywords:  Acute lung injury; Acute respiratory distress syndrome; G protein-coupled receptor 161; Glycolytic reprogramming; Inflammatory response; Macrophage
    DOI:  https://doi.org/10.1186/s11658-026-00955-3
  34. Mol Immunol. 2026 May 23. pii: S0161-5890(26)00128-8. [Epub ahead of print]195 198-208
      Acute respiratory distress syndrome (ARDS), the most severe presentation of acute lung injury (ALI), involves diffuse pulmonary inflammation and edema, with hypoxemic respiratory failure as the end result. Given the high mortality and limited therapeutic options, the development of novel interventions is urgently needed. Effective control of excessive pulmonary inflammation and modulation of alveolar macrophage polarization play critical roles in disease progression. In this study, we investigated the therapeutic potential of uridine in alleviating ALI. An in vivo model was established by intratracheal administration of lipopolysaccharide (LPS) in mice, and in vitro experiments were performed using LPS-stimulated MH-S cells. Our results demonstrated that uridine treatment significantly attenuated LPS-induced lung injury, as evidenced by reduced lung index (LI), wet-to-dry weight ratio (W/D), myeloperoxidase (MPO) activity, protein leakage and pro-inflammatory cytokine levels in lung tissues and bronchoalveolar lavage fluid (BALF), along with improved survival in mice. Mechanistically, uridine‑induced M2 polarization of alveolar macrophages was accompanied by STAT6 phosphorylation, suggesting a potential involvement of this pathway. Collectively, these findings suggest that uridine may serve as a promising adjunctive therapeutic agent for ALI, potentially improving disease outcomes through the induction of M2 macrophage polarization.
    Keywords:  Acute lung injury; Anti-inflammation; Macrophage polarization; STAT6; Uridine
    DOI:  https://doi.org/10.1016/j.molimm.2026.05.009
  35. Front Immunol. 2026 ;17 1815436
      The innate immune system of fish has conventionally been considered incapable of immunological memory, but is now being recognised as exhibiting memory-like features known as trained immunity. This study investigated the induction of trained immunity in Atlantic salmon (Salmo salar L.) by training head kidney-derived leukocytes using β-glucan, followed by a resting phase and secondary stimulation with β-glucan (homologous) or lipopolysaccharide (LPS, heterologous). The cellular responses, metabolite production, and gene expression related to innate immunity, metabolism, and epigenetic markers were assessed. The effects of initial β-glucan training persisted after a 5-day resting period, during which upregulation occurred in the expression of key innate immune and metabolic genes. Upon secondary stimulation, leukocytes exhibited stimulus-dependent transcriptional responses with increased expression of several pro-inflammatory and metabolic genes, particularly in the heterologous LPS-exposed group. Attenuation of specific inflammatory cytokine responses occurred in trained cells upon LPS stimulation, but metabolic gene expression patterns indicated regulation toward enhancing glycolytic activity and mitochondrial oxidative metabolism. Trained cells also displayed significantly increased phagocytic activity, especially after heterologous exposure. Only minor or moderate changes occurred in other cellular outputs (reactive oxygen species, nitric oxide, lactate, and fumarate). Epigenetic markers showed limited expression changes. The experimental evidence indicates a phenotype similar to trained immunity in salmon leukocytes, characterised by transcriptional and functional alterations following β-glucan training; however, responses vary upon secondary exposure to a heterologous stimulus. This study provides new insight into trained immunity in Atlantic salmon by demonstrating the transcriptional and cellular response of leukocytes to develop stimulus-dependent immune and metabolic regulations.
    Keywords:  aquaculture; immunological memory; immunostimulant; phagocytosis; trained immunity
    DOI:  https://doi.org/10.3389/fimmu.2026.1815436
  36. Cell Death Dis. 2026 May 25. pii: 506. [Epub ahead of print]17(1):
      Insulin signaling regulates cellular metabolism in an epigenetic manner, but its role in the immune cell homeostasis remains unknown. High plasma insulin obstructs efficient insulin signaling and rewires metabolic activity in autoimmunity. In this study, we explored the functional consequences of insulin signaling for the metabolism and phenotype of effector CD4+ T cells in blood and synovial tissue of patients with rheumatoid arthritis (RA). Transcriptome profiling of CD4+ cells in RA blood and synovia revealed high metabolic activity and effector function of the survivin/BIRC5hiPD1hi T peripheral helper cell population. Low insulin signaling and deficient histone acetylation in RA T cells amplified proinflammatory IFNγ and TNF expression. Co-deposition of survivin with acetylated histone H3K27 on regulatory chromatin controlled the transcription of histone acetylation complex subunits and insulin-dependent genes. Insulin stimulation and histone deacetylase inhibition induced an increase in histone acetylation. In CD4+ cell cultures and in aggressive PD1hiTph cells in RA synovial tissue, exposure to insulin synergized with inhibition of histone deacetylation to upregulate IL7 production suppressing IFNγ and PD1. This activated IL7R-signaling mediators STAT5A/B, BCL2, and promoted acquisition of CD27+CD45RO+ central memory phenotype in the PD1hiTph cells. Likewise, the CD4+ cells in hyperinsulinemic T2D patients showed enrichment of IL7R+T cell cluster. In RA patients, antagonizing folate transport and JAK/STAT signaling activated insulin signaling and histone acetylation-dependent metabolism of CD4+ cells. Concomitant with CTLA4-dependent signaling, this enabled the adoption of an incipient IL7R+ T cell phenotype. This study demonstrates that insulin binds together metabolic activity and histone acetylation in CD4+ cells. Sufficient insulin signaling promotes IL7R+ memory phenotype accrual in aggressive PD1hiTph cells. Hence, achieving insulin sensitivity via histone acetylation disarms effector CD4+ T cell function and presents an attractive interventional goal to restore immune cell homeostasis in RA.
    DOI:  https://doi.org/10.1038/s41419-026-08916-6