bims-lycede Biomed News
on Lysosome-dependent cell death
Issue of 2025–07–13
two papers selected by
Sofía Peralta, Universidad Nacional de Cuyo
  1. Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi.
  2. Real-Time Monitoring of Adenosine Triphosphate Fluctuation in Lysosome during Autophagy/Mitophagy.
  1. Elife. 2025 Jul 08. pii: RP98582. [Epub ahead of print]13
    Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi.
    Hieng Chiong Tie, Haiyun Wang, Divyanshu Mahajan, Hilbert Yuen In Lam, Xiuping Sun, Bing Chen, Yuguang Mu, Lei Lu.
      How the intra-Golgi secretory transport works remains a mystery. The cisternal progression and the stable compartment models have been proposed and are under debate. Classic cisternal progression model posits that both the intra-Golgi transport and Golgi exit of secretory cargos should occur at a constant velocity dictated by the cisternal progression; furthermore, COPI-mediated intra-Golgi retrograde transport is essential for maintaining the Golgi organization. Leveraging our recently developed Golgi imaging tools in nocodazole-induced Golgi ministacks, we found that the intra-Golgi transport velocity of a secretory cargo decreases during their transition from the cis to the trans-side of the Golgi, and different cargos exhibit distinct velocities even within the same cisternae. We observed a vast variation in the Golgi residence times of different cargos. Remarkably, truncation of the luminal domain causes the Golgi residence time of Tac - a standard transmembrane secretory cargo without intra-Golgi recycling signals - to extend from 16 min to a notable 3.4 hr. Additionally, when COPI-mediated intra-Golgi retrograde transport was inhibited by brefeldin A, we found that nocodazole-induced Golgi can remain stacked for over 30-60 min. Therefore, our findings challenge the classical cisternal progression model and suggest the stable compartment nature of the Golgi.
    Keywords:  Golgi; cell biology; cisternal progression model; human; intra-Golgi transport; membrane trafficking; stable compartment model
    DOI:  https://doi.org/10.7554/eLife.98582
  2. ACS Appl Mater Interfaces. 2025 Jul 11.
    Real-Time Monitoring of Adenosine Triphosphate Fluctuation in Lysosome during Autophagy/Mitophagy.
    Jiwen Hu, Hong Wang, Xin Zhang, Chunfei Wang, Anna du Rietz, Mengtao Rong, Caroline Brommesson, Xiongyu Wu, Zhanxiao Wei, Ruilong Zhang, Xuanjun Zhang, Kajsa Uvdal, Zhangjun Hu.
      Autophagy, a lysosomal degradation pathway critical for cell survival, differentiation, development, and maintaining homeostasis, plays a crucial role in cellular health. Maintaining an adequate level of adenosine triphosphate (ATP), the central molecule in energy metabolism, is equally essential for these processes. However, the interplay between autophagy and energy metabolism remains incompletely understood due to technical challenges, including the need for high-precision, dynamic detection within organelles, and sensitivity to the acidic lysosomal environment. To address these limitations, we developed HR-MP, a ratiometric fluorogenic nanoprobe specifically designed for visualizing ATP levels in acidic lysosomes during autophagy. HR-MP exhibits selective, rapid, and quantitative ATP detection in vitro, allowing it to quantitatively monitor lysosomal ATP fluctuations in complex biological environments with excellent biocompatibility, membrane permeability, and lysosome-targeting ability. Importantly, HR-MP enables real-time tracking of ATP fluctuations during starvation- or drug-induced autophagy in living cells, providing a powerful tool for elucidating the links between autophagy and energy metabolism.
    Keywords:  ATP monitoring; Förster resonance energy transfer (FRET); lysosome targeting; ratiometric fluorogenic nanoprobe; silica nanoparticles
    DOI:  https://doi.org/10.1021/acsami.5c07496
This page is being maintained by Thomas Krichel. It was last updated on 2025‒07‒13 at 13:06.