bims-lycede Biomed News
on Lysosome-dependent cell death
Issue of 2025–11–16
two papers selected by
Sofía Peralta, Universidad Nacional de Cuyo
  1. The Impact of Golgi Organization on Cell Cycle and Cancer Development.
  2. Transcription Factor EB (TFEB) Expression and Localization in the Third-Trimester Placenta.
  1. Subcell Biochem. 2026 ;110 109-148
    The Impact of Golgi Organization on Cell Cycle and Cancer Development.
    Antonino Colanzi, Inmaculada Ayala.
      The Golgi complex plays a pivotal role in the secretory pathway, cell signaling, and cytoskeleton organization. In mammalian cells, it is organized as a ribbon-like structure that can undergo dynamic reorganizations, which are crucial for various cellular processes, including mitosis and cell migration. During mitosis, the Golgi complex is subjected to a tightly regulated disassembly process, essential for proper cell division, while its reformation is critical for inheritance by daughter cells. Disruption of Golgi organization has been linked to pathological conditions, including cancer, as altered organization and function can contribute to tumor progression by impacting glycosylation, cell polarity, and extracellular matrix remodeling. This chapter explores the molecular mechanisms regulating Golgi disassembly during the cell cycle and its involvement in ensuring proper cell division. Additionally, we discuss the emerging understanding of the potential role of modifications of the Golgi organization in tumor development and metastasis. Collectively, the findings presented in this chapter underscore the physiological significance of the Golgi ribbon structure and the pathological implications of its alterations in cancer progression, which could offer novel therapeutic opportunities for cancer treatment.
    Keywords:  Centrosome; Checkpoint; Golgi complex; Mitosis; Mitotic spindle; Ribbon
    DOI:  https://doi.org/10.1007/978-3-032-06936-8_6
  2. Int J Mol Sci. 2025 Oct 22. pii: 10294. [Epub ahead of print]26(21):
    Transcription Factor EB (TFEB) Expression and Localization in the Third-Trimester Placenta.
    Cinzia Giacometti, Alessandro Ambrosi, Serena Cavaliere, Anna Caliò, Daniele Mautone, Guido Martignoni.
      Transcription factor EB (TFEB) is expressed at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization. Preeclampsia (PE) is a severe complication of pregnancy with a high incidence of maternal and fetal morbidity and mortality. Gestational diabetes (GD) is a metabolic disease that can affect placental villous maturation and villous vascularity. We analyzed the expression of three different antibodies: TFEB from Invitrogen (TFEB-INV), which detects endogenous levels of TFEB only when phosphorylated at Ser211; TFEB from Bethyl Labs (TFEB-B), which recognizes and binds E-box sequences; and TFEB from Santa Cruz (C-6) (TFEB-SC), which is specifically used for epitope mapping between 440 and 470. We evaluated the presence/absence of TFEB in six placental districts: syncytiotrophoblast (STB), cytotrophoblast (CTB), extravillous trophoblast (EVT), syncytial knots, stem villi vessels, and villous capillaries. TFEB-B was significantly expressed in the stem villi vessels, STB, and villi vessels of GD cases. The lack of TFEB expression in late-onset PE appears to corroborate the role of TFEB in vascular remodeling during placental development. The positive results in STB and vessels in GD cases, regardless of the histological diagnosis, may suggest that the expression of TFEB mitigates hypoxic injury via the Akt/mTOR pathway.
    Keywords:  TFEB; gestational diabetes; immunohistochemistry; preeclampsia
    DOI:  https://doi.org/10.3390/ijms262110294
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