bims-medebr Biomed News
on Metabolism of the developing brain
Issue of 2026–06–07
28 papers selected by
Regina F. Fernández, Johns Hopkins University



  1. J Neurochem. 2026 Jun;170(6): e70467
      This Preface introduces the Special Issue entitled, "Brain Bioenergetics in Aging: Neurovascular and Neurometabolic Coupling and Fuels," which is comprised of manuscripts contributed by invited speakers and program/organizing committee members who participated in the 15th International Conference on Brain Energy Metabolism (ICBEM) held on September 17-21, 2024, in Ljubljana, Slovenia. The conference covered the latest developments in research related to (i) coordination of neurometabolic and neurovascular coupling and homeostasis of energy metabolism in healthy aging and Alzheimer's disease, (ii) in vivo imaging modalities for study of neurometabolic and neurovascular coupling, (iii) mitochondrial and metabolic alterations and resilience in injured and aging brain, (iv) astrocyte metabolism in Alzheimer's and other neurodegenerative diseases, (v) microglial support of neuronal metabolism and role in neurodegeneration, (vi) neuronal mitochondria and disease, (vii) lipids and transporters in brain function, metabolism and Alzheimer's disease, and (viii) metabolic regulation of cognition. The special issue contains 19 manuscripts on these topics.
    DOI:  https://doi.org/10.1111/jnc.70467
  2. Metab Brain Dis. 2026 May 30. pii: 121. [Epub ahead of print]41(1):
      Acyl-CoA synthetase long-chain family member 6 (ACSL6) is a member of the long-chain acyl-CoA synthetase (ACSLs) family that is particularly expressed in nervous system. It mainly catalyzes the activation reaction of polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA), providing substrates for the synthesis and remodeling of neuronal membrane lipids. Recent studies have shown that ACSL6 plays a decisive role in DHA enrichment, synaptic plasticity and antioxidant defense in the brain. Its dysfunction can lead to changes in membrane lipid composition, weakened synaptic signals and excessive activation of neuroinflammation, thereby causing neurological deficits like cognitive and motor disorders. This review comprehensively summarizes the molecular structure characteristics and catalytic mechanism of ACSL6, and analyzes the roles of its different domains in substrate recognition and reaction regulation. ACSL6 participates in lipid metabolism by converting DHA into DHA-CoA, forming a local DHA metabolic pathway and providing continuous energy supply for the structural stability and signal transmission of nerve membranes. The localization characteristics of ACSL6 enable the spatial directional distribution of DHA in the synaptic membrane and endoplasmic reticulum regions, which is a key link in maintaining brain lipid homeostasis. In addition, ACSL6 is involved in the defense mechanisms of the nervous system by regulating oxidative stress responses, ferroptosis and inflammatory pathways. Its dysregulation has been confirmed to be associated with various neurodegenerative diseases. A thorough clarification of the molecular mechanism of ACSL6 may provide a new theoretical basis and highlight potential avenues for future therapeutic exploration regarding the imbalance of lipid homeostasis in neurons and related diseases.
    Keywords:  ACSL6; DHA; Lipid metabolism; Neurodegenerative diseases; Neuron
    DOI:  https://doi.org/10.1007/s11011-026-01886-w
  3. Mech Ageing Dev. 2026 Jun 01. pii: S0047-6374(26)00058-8. [Epub ahead of print] 112206
      Ageing is the primary risk factor for neurodegeneration and age-related cognitive decline, which is increasingly recognised as a systemic collapse of metabolic crosstalk between neurons and glial cells in the brain. This narrative review elucidates that mitochondrial dynamics - encompassing biogenesis, fusion, fission, and mitophagy - acts as the core regulatory mechanism governing this multicellular interaction network, and drives the cell-specific energy crisis that underpins pathological progression in the ageing brain. We delineate that senescent astrocytes disrupt the astrocyte-neuron lactate shuttle, oligodendrocytes develop ATP deficits triggering myelin breakdown, and microglia undergo maladaptive immunometabolism and metabolic reprogramming via excessive Drp1-mediated mitochondrial fission, which collectively initiates and amplifies chronic neuroinflammation and neurodegenerative damage. Crucially, we highlight intercellular mitochondrial transfer as a vital endogenous rescue mechanism, wherein glial cells donate functional mitochondria to stressed neurons to mitigate damage. Finally, we synthesise emerging therapeutic strategies targeting the glia-neuron mitochondrial social network, providing a holistic framework for restoring brain bioenergetic homeostasis and delaying age-related neurodegenerative progression.
    Keywords:  Cell-Specific Energy Crisis; Immunometabolism; Intercellular Mitochondrial Transfer; Metabolic Reprogramming; Mitochondrial Dynamics
    DOI:  https://doi.org/10.1016/j.mad.2026.112206
  4. Magn Reson Med. 2026 May 30.
       PURPOSE: Deuterium metabolic imaging (DMI) offers noninvasive magnetic resonance imaging (MRI)-based assessment of metabolism in vivo, making it a relevant paraclinical tool for diseases with neurological metabolic alterations. This study aimed to establish a normative reference atlas of brain glucose metabolism accounting for age and sex.
    METHODS: DMI were obtained for 30 healthy adults (aged 51-84 years, 15 female) with a 3 T MRI scanner after ingestion of deuterated [6,6´-2H2]glucose. The images were parcellated to determine the regional distribution of deuterated water, glucose, lactate, and glutamate plus glutamine (Glx). Linear models were applied to investigate the effects of age, sex, and other exploratory adjustments. As a proof-of-concept example of atlas application, the normative atlas was compared with patients with Alzheimer's disease and healthy subjects from a previous study.
    RESULTS: Regional differences were significant for all metabolites (p < 10-15), with the highest values in the occipital lobes, except for lactate, whose regional distribution pattern was less consistent. While lactate production showed no overall age-dependency, global Glx production decreased 13% ± 4% per decade. Lactate production tended to be higher in males than females (p = 0.042), but this was not significant after regional adjustment (p = 0.084). Discriminating between health and Alzheimer's disease required additional adjustments for weight, blood glucose, and timing.
    CONCLUSIONS: While regional and age effects explained a substantial part of the variability in Glx, reliable intersubject comparisons required additional adjustments. The normative atlas presented here provides a reference for future DMI studies of brain metabolism.
    Keywords:  Alzheimer's disease; atlas; brain; deuterium; glucose metabolism; magnetic resonance imaging
    DOI:  https://doi.org/10.1002/mrm.70451
  5. J Biomed Sci. 2026 Jun 01. pii: 57. [Epub ahead of print]33(1):
       BACKGROUND: Dysregulated interferon-alpha/beta-receptor 1 (IFNAR1) signaling was recently identified to contribute to the development of sporadic Parkinson's disease (PD) into PD with Dementia (PDD). The molecular, cellular, and phenotypic impacts of brain IFNAR1 loss in aging have not been explored in vivo, which may reveal novel disease mechanisms and therapeutic targets.
    METHODS: Single nuclei RNA sequencing (snRNA-seq), liquid chromatography tandem mass spectrometry (LC-MS/MS), functional metabolic mapping, flow cytometry, quantitative PCR (qPCR), in situ hybridization, immunofluorescence and immunohistochemistry, Western blotting, and behavior analyses were used to investigate the molecular, cellular, and phenotypic impacts of IFNAR1 loss in vivo.
    RESULTS: Baseline IFNAR1 expression varies among major brain cell types, including neurons and astrocytes, and is differentially affected in PD and Lewy Body Dementia patients compared to unaffected controls. Neuron- and astrocyte-specific transcriptomic and proteomic alterations in Ifnar1-/- mice implicate mitochondrial defects, defective mitophagy, and synergistic dysfunctional neurotransmission upon IFNAR1 loss, leading to glucose hypermetabolism measured by functional metabolic analysis. Consequently, Ifnar1-/- mice exhibited PDD-like pathogenesis, including dopaminergic cell loss in the substantia nigra, cortical neurodegeneration, Lewy-body-like inclusions, neuroinflammation, and progressive PDD-like behavior deficits. Brain cell-specific IFNAR1 loss examined in vivo revealed delayed but distinct development of PDD-like phenotypes, where neuropathology, motor, and cognitive behavior deficits were recapitulated only in mice lacking neuronal IFNAR1, and behavior resembling neuropsychiatric abnormalities recapitulated only in mice lacking astrocytic IFNAR1.
    CONCLUSIONS: IFNAR1 plays a crucial role in brain and mitochondrial homeostasis, loss of which results in neurodegeneration and neuropathology resembling PDD. Differential neuropathology and behavioral outcomes upon neuronal vs astrocytic IFNAR1 loss emphasizes a need for understanding neurodegenerative pathophysiology in cell-specific contexts. Trial registration Not applicable as the study does not include a clinical trial.
    Keywords:  Brain energy metabolism; IFNAR1; LC–MS/MS; Mitochondria; Parkinson’s disease dementia; SnRNA-seq
    DOI:  https://doi.org/10.1186/s12929-026-01257-8
  6. J Neurotrauma. 2026 Jun 03. 8977151261452922
      Traumatic brain injury (TBI) affects millions globally each year, often resulting in long-term health issues or death. While the immediate physical damage caused by these injuries receives much attention, subsequent metabolic changes in the brain are equally vital to recovery but understudied. After TBI, brain energy regulation and consequential metabolic processes are disrupted. This review provides a detailed examination of metabolic alterations following TBI, including glucose and lipid processing disruptions, increased lactate levels, neurotransmitter imbalances, and oxidative stress. These changes can lead to hyper/hypoglycemia, lactate accumulation, chemical imbalances, and heightened oxidative stress, all of which impede recovery. Understanding these biochemical shifts is essential for developing more effective treatments. This review offers a comprehensive overview of brain metabolic changes post-TBI and discusses some promising therapies, including drugs, nutrition, and lifestyle adjustments, that could aid recovery and improve the quality of life of those impacted.
    Keywords:  brain injury; excitotoxicity; glucose; metabolism; neuroinflammation; neurotransmission
    DOI:  https://doi.org/10.1177/08977151261452922
  7. J Nutr. 2026 Jun 03. pii: S0022-3166(26)00279-8. [Epub ahead of print] 101630
       BACKGROUND: Energy deficits underlie many neurodevelopmental, neuropsychiatric and neurodegenerative diseases implicating mitochondria as a potential therapeutic target. Iron is necessary for neuronal energy output through its direct role in mitochondrial oxidative phosphorylation. Iron deficiency (ID) reduces mitochondrial energetic capacity in developing hippocampal neurons and causes simplified dendritic arbors and impaired learning and memory.
    OBJECTIVE: To determine the effect of ID on axonogenesis, which has not been previously explored.
    METHODS: We used an embryonic mouse mixed-sex primary hippocampal neuron culture model of developmental ID, using iron chelation with low micromolar deferoxamine (DFO) from 3 days in vitro (DIV) to 7DIV compared to untreated control cultures. Mitochondrial respiration and dynamics, cytoskeletal and metabolic gene expression, and axonal and synaptic morphology were quantified and compared using t-test, ANOVA, and multivariate statistical analyses.
    RESULTS: 7DIV DFO-treated neuron cultures (n=4-17) demonstrated moderate ID with significantly decreased mRNA levels for genes involved in axon cytoskeletal development (Gda, Pfn2, and Nuak1; ∼20-40% lower) and metabolic homeostasis (Ndufs1, Ddit4, and Slc2a3; ∼20-25% lower). DFO significantly reduced total ATP production rate and measures of mitochondrial oxidative phosphorylation by ∼25-50% compared to control cultures (n=11-14). DFO significantly reduced the length of the primary axon and axonal branches by ∼20%, without affecting branch number (n=100 neurons). Axonal mitochondrial motility was not altered by ID (n=11-12 neurons), suggesting that impaired mitochondrial energetics, and not trafficking, is the predominate mitochondrial contribution to axon morphological deficits. Ultimately, at 18DIV, DFO significantly reduced the density of post-synaptic density puncta, a measure of neuronal capacity for synapse formation, by 30% (n=26-32 neurons).
    CONCLUSIONS: These findings provide the first link between iron-dependent neuronal energy production and early axon structural development and highlight the importance of maintaining sufficient iron during the embryonic period of rapid axonal growth to prevent the persistent negative consequences of ID on neuronal structure.
    Keywords:  axon; axonogenesis; energy metabolism; gene expression; iron deficiency; mitochondria; mitochondrial motility; mitochondrial trafficking; neuron development
    DOI:  https://doi.org/10.1016/j.tjnut.2026.101630
  8. Eur J Nucl Med Mol Imaging. 2026 May 30.
       PURPOSE: Cholesterol 24-hydroxylase (C24H) is central to brain cholesterol metabolism and is thought to be altered in many neurodegenerative diseases, including Alzheimer's disease (AD). Only two fluorinated C24H-targeting PET radioligands, [¹⁸F]CHL-2205 and [¹⁸F]T008, have recently entered clinical use. Direct comparison is needed to guide radiotracer selection for future translational studies.
    METHODS: Dynamic PET imaging was performed using either [¹⁸F]CHL-2205 or [¹⁸F]T008 in parallel conditions in cynomolgus macaques, healthy rats, the TgF344-AD rat model, and C24H-overexpressing mice. Arterial blood sampling enabled full kinetic modeling in healthy rats and nonhuman primates. An image-derived input function was validated in rats, and the specific binding was evaluated under C24H saturation using soticlestat.
    RESULTS: [¹⁸F]CHL-2205 and [¹⁸F]T008 showed similar metabolism profiles in either macaques or rats. In macaques, brain uptake values (VT, Logan plot analysis) were 1.6-fold higher for [¹⁸F]CHL-2205 relative to [¹⁸F]T008. Healthy rats showed a similar pattern, with [¹⁸F]CHL-2205 exhibiting 1.5-fold higher brain VT. In rats, soticlestat blockade revealed C24H-specific binding for both ligands, with an estimated binding potential (BPND) 1.3-fold higher for [¹⁸F]CHL-2205 compared to [¹⁸F]T008. In TgF344-AD rats, brain uptake increased by 1.9-fold with [¹⁸F]CHL-2205 versus 1.6-fold with [¹⁸F]T008 compared to wild-type controls. In C24H-overexpressing mice, [¹⁸F]CHL-2205 detected measurable increases in brain signal in several brain regions (up to 1.5-fold), while [¹⁸F]T008 did not in the same animals.
    CONCLUSION: Across species and models, both radioligands demonstrated appropriate characteristics for imaging C24H in vivo. However, [¹⁸F]CHL-2205 generally displayed higher specific binding and appeared more sensitive to detect moderate increases in C24H expression.
    Keywords:  Alzheimer’s disease; CYP46A1; Cholesterol; Lipid metabolism; Neuroimaging; PET imaging; [¹⁸F]CHL-2205; [¹⁸F]T008
    DOI:  https://doi.org/10.1007/s00259-026-07937-9
  9. Neuropsychopharmacology. 2026 May 30.
      Alcohol consumption elevates circulating acetate. Prior studies showed that acute alcohol reduces brain glucose uptake and increases brain acetate oxidation. Previously we showed that heavy drinkers have elevated capacity to oxidize brain acetate. Here we repeat the study, adding individuals with alcohol use disorder (AUD). Four groups were enrolled. The analysis data set included Light Drinkers (LD, n = 13, female = 5), at-risk Heavy Drinkers (HD, n = 15, female = 7), AUD patients in long-term recovery (≥6 months; AUDLTR, n = 6, female = 1), and a separate group of AUD treatment-seekers (AUDTx, n = 12, female = 1) underwent medically supervised detoxification, scanned at ~1 week abstinence (n = 9) and 1 month (n = 10). Seven AUDTx participants successfully completed scans at both time points. We infused participants with [2-13C]acetate during magnetic resonance spectroscopy (MRS) of 13C-glutamate (Glu) and 13C-glutamine (Gln) in the brain, to measure the cerebral metabolic rate for acetate (CMRAc) and the neuronal tricarboxylic acid cycle relative to glutamate-glutamine neurotransmitter cycling (VtcaN/Vcycle; Energy Per Cycle: EPC). There was a group effect for CMRAc (p = 0.007) primarily owing to lower CMRAc in AUDTx at 1 week. Furthermore, higher CMRAc was observed among HD compared to LD participants, as previously reported. CMRAc was similar between the AUDLTR and HD groups. In a separate within-subject comparison among AUDTx participants, CMRAc increased after 1 month to levels similar to those of LD. EPC was similar among the groups, representing normal glutamate-glutamine cycling versus energetics. In summary, abstinence reversed the lower acetate oxidation in early AUD, showing that just a few weeks of recovery can normalize this metabolic abnormality.
    DOI:  https://doi.org/10.1038/s41386-026-02455-6
  10. Mech Ageing Dev. 2026 Jun 02. pii: S0047-6374(26)00061-8. [Epub ahead of print] 112209
      Cellular senescence plays a crucial role in brain aging and the decline of cognitive abilities. Although senolytic treatments, particularly the combination of dasatinib and quercetin (D+Q), show potential for improving cognition, the specific mechanisms involved are not well understood. In this study, we present strong evidence that senolytics enhance cognitive function by modulating cholesterol biosynthesis in astrocytes. Using a murine model of accelerated aging (via D-galactose exposure), we show that administering D+Q results in significant improvements in cognitive performance and a decrease in cellular senescence. A comprehensive multi-omics analysis subsequently demonstrated that senolytics are especially effective at downregulating cholesterol biosynthesis in the hippocampus. Notably, this effect was localized to astrocytes, where senolytics significantly reduced intracellular lipid accumulation and subsequent neuroinflammation. Critically, pharmacological activation of cholesterol synthesis and genetic overexpression of Hsd17b7, a key enzyme in the cholesterol synthesis pathway, in astrocytes reversed the anti-senescence benefits of senolytics in vitro, establishing a causal link between cholesterol pathway inhibition and the therapeutic effects. Overall, our work identifies the suppression of astrocytic cholesterol biosynthesis as a fundamental mechanism of senolytic action, repositioning these compounds as modulators of brain lipid metabolism and highlighting a promising therapeutic axis for combating age-related cognitive decline.
    Keywords:  Astrocytes; Cellular Senescence; Cholesterol Metabolism; Cognitive Decline; Senolytics
    DOI:  https://doi.org/10.1016/j.mad.2026.112209
  11. J Cereb Blood Flow Metab. 2026 Jun 01. 271678X261455750
      [18F]FDG functional PET (fPET) enables investigation of dynamics in glucose metabolism occurring within seconds. However, the physiological mechanisms supporting rapid metabolic changes necessitate further attention to allow accurate interpretation. This work highlights candidate mechanisms driving [18F]FDG signal changes at high temporal resolution, offering complementary insights to existing interpretations.At rest, metabolic demands are closely matched by glucose supply across the blood-brain barrier (BBB), regulated by glucose transporter 1 (GLUT1). During neuronal activation, glucose transport and phosphorylation by hexokinase are elevated to meet increased energy requirements. Simulations indicate that rapid [18F]FDG signal increases are primarily driven by BBB transport, with subsequent increases in hexokinase activity. Mechanisms supporting increased BBB transport include elevated glucose concentration gradient towards the brain and changes in GLUT1 intrinsic properties, but only minor effects of blood flow. Conversely, moment-to-moment fluctuations in [18F]FDG used for metabolic connectivity, reflect temporally synchronized supply, mediated jointly by blood flow and BBB transport.We emphasize that the coupling between BBB transport and metabolism underpin the [18F]FDG fPET signal. Considering alterations of GLUT1 and subsequent metabolism in numerous brain disorders, stimulation-induced energy demands and metabolic connectivity represent a promising opportunity to investigate the underlying pathophysiological processes.
    DOI:  https://doi.org/10.1177/0271678X261455750
  12. Lipids Health Dis. 2026 Jun 04.
       BACKGROUND: Cholesterol is a crucial determinant of membrane structure, signaling, and synaptic function in the central nervous system, where its homeostasis is tightly controlled to support neuronal viability and plasticity to prevent neurodegeneration. Altered brain cholesterol metabolism is increasingly recognized as a shared feature of several neurological disorders as well as age-associated cognitive decline. In parallel, neuroglobin has emerged as an endogenous neuroprotective protein in the brain, where its overexpression limits oxidative damage, mitochondrial dysfunction, and cell death in experimental models of hypoxia, ischemia, and proteinopathy. Despite extensive research on cholesterol turnover in the brain and on neuroglobin-mediated cytoprotection, the functional relationship between these two processes remains largely unexplored. The purpose of this study was to investigate whether neuroglobin modulates the cholesterol regulatory network in brain-derived cells and whether it affects the astrocyte-dependent cholesterol supply to neurons.
    METHODS: Human neuronal-like (SH-SY5Y) and astrocyte-like (U373) cell lines overexpressing neuroglobin were used to examine the effects of globin on key regulators of cholesterol synthesis, uptake, intracellular trafficking, and storage, as well as on susceptibility to oxidative stress. Western blot and immunofluorescence analyses were performed.
    RESULTS: The results demonstrate that neuroglobin is seemingly associated with changes in cholesterol homeostasis in a cell type-specific manner, modulating the expression of different proteins involved in cholesterol metabolism and promoting cellular cholesterol accumulation without affecting the cross-talk between astrocytes and neurons.
    CONCLUSIONS: Overall, these findings suggest that NGB can be a novel modulator of cholesterol homeostasis in brain-derived cells, extending its role beyond the mitigation of oxidative stress in neurons to include defense against metabolic dysregulation.
    Keywords:  Astroglia-like cells; Cholesterol; Metabolism; Neuroglobin; Neuron-like cells; Rotenone
    DOI:  https://doi.org/10.1186/s12944-026-02985-4
  13. Biochemistry (Mosc). 2026 May;91(5): 623-636
      This special issue of Biochemistry (Moscow) "Interaction between Neural Signals and Metabolic Pathways: Role in the Functioning of a Healthy and Diseased Brain", includes studies on the mechanisms of close functional connections between the brain and other organs and tissues of the body. These mechanisms link brain metabolism with its signaling function under normal and pathological conditions. The metabolic signals that enable these connections are the focus of research in this field, which is crucial for an integrated understanding of how the body functions. An impairment in metabolic signaling leads to the development of various pathologies. Metabolites such as glucose, fatty acids, and amino acids act as primary signals that influence neural networks and brain chemistry. This connection between the body's metabolism and brain signaling is not merely a matter of fuel supply, but rather a complex information exchange process. The interaction between the brain and the body occurs within the framework of coordinated work of two main axes: the brain-to-body axis ("from top to bottom" or from center to periphery), and the body-to-brain axis (from periphery to center). This relationship between brain function and body metabolism forms a mechanical and logical connection between metabolic somatic diseases and brain disorders that may underlie their comorbidities. The close connection between brain function, metabolism, and the metabolism of peripheral organs and tissues forms the basis for treating "body-brain metabolic" disorders. Identifying the molecular and cellular mechanisms underlying this relationship allows identifying targets for treating and preventing comorbid somatic and brain conditions. The recent achievements, which prove the close relationship between metabolism and brain activity, have led to the emergence of a rapidly growing interdisciplinary field at the interface of neuroscience, philosophy of consciousness, and functional biochemistry of metabolism. This new synthetic field can be called "metabolic neurophilosophy". Its subject is to explore the integrity and inseparability of the body's metabolism (including both in the brain and peripheral organs and tissues) and the signaling and informational function of the brain. It also studies the dependence of all brain activity, including cognition, and mental states on energy processes and metabolic signaling throughout the body.
    Keywords:  brain; brain diseases; comorbidity; energy balance; mental disorders; metabolic disorders; metabolism; neurodegenerative diseases; neuroendocrine system; neurological diseases; neurophilosophy; somatic diseases; stress; synaptic plasticity; visceral organs
    DOI:  https://doi.org/10.1134/S0006297926601450
  14. Neuroimage Clin. 2026 May 27. pii: S2213-1582(26)00073-2. [Epub ahead of print]50 104014
      Aging is accompanied by several neurophysiological changes. Of recent interest are age-related changes in brain energetics-how the brain produces and uses energy. In vitro, preclinical, and genetic investigations point to a general decline in the efficiency of brain energetics in aging, which may help explain age-related changes in mood, cognition, movement, and behaviour, as well as an increased likelihood of developing neurodegenerative disorders. We hypothesized that age-related alterations to brain energetics are observable using functional neuroimaging data from healthy individuals (N = 24, 35-80 years), and that alterations may occur in regions associated with neurodegenerative pathology. Specifically, we jointly analyzed cerebral glucose metabolism and blood flow data, defining novel metrics that capture regional variability in processes related to relative energy production (rEP) and relative aerobic glycolysis (rAG), an energy production mechanism. By applying Scaled Subprofile Modeling Principal Component Analysis (SSM-PCA) to these metrics, we identified spatial covariance patterns that capture a widespread age-related restructuring of brain energetics. Broadly, an age-related rEP pattern (rage2 = 0.72) consisted of rEP increases in frontal, basal ganglia, and brainstem regions, coupled with decreases in occipitoparietal regions, while an age-related rAG pattern (rage2 = 0.65) revealed rAG increases in substantia nigra and occipitoparietal regions and decreases in caudate and frontal regions. Finally, by comparing these patterns to well-known metabolic patterns related to Parkinson's and Alzheimer's disease, we identified the strongest topological similarity and covarying pattern expression between the age-related rEP pattern and the Parkinson's Disease Related Pattern (PDRP). These results provide in vivo support for altered brain energetics with age and potential neurophysiological similarities between aging and neurodegeneration.
    Keywords:  Aerobic glycolysis; Brain energetics; Healthy aging; Neurodegeneration; PET/MRI; Spatial covariance analysis
    DOI:  https://doi.org/10.1016/j.nicl.2026.104014
  15. Proc Natl Acad Sci U S A. 2026 Jun 09. 123(23): e2610646123
      In peripheral tissues, lipoprotein lipase (LPL) is secreted by parenchymal cells (adipocytes, myocytes) into the interstitial spaces, where it is captured by GPIHBP1 (a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells) and escorted to the luminal surface of capillaries. The LPL inside capillaries hydrolyzes glycerolipids in the plasma lipoproteins, releasing fatty acids for parenchymal cells. In the central nervous system, LPL is synthesized by multiple cell types [e.g., microglia, oligodendrocyte precursor cells (OPCs)] and secreted into the interstitium, but a binding site for the LPL has never been identified. By examining single nuclei RNA-seq databases of the human brain, we found that GPIHBP1 is expressed by oligodendrocytes but not by OPCs. This gene-expression profile (high in oligodendrocytes, low in OPCs) is also observed in genes for myelin structural proteins, fatty acid binding and transport proteins, and lipid biosynthetic enzymes. GPIHBP1 expression in oligodendrocytes was confirmed by in situ hybridization studies of human brain and by immunohistochemical staining. Of note, GPIHBP1 and LPL are colocalized on oligodendrocytes in the human brain. Our findings identify GPIHBP1 as a principal binding site for interstitial LPL in the human brain and suggest that GPIHBP1-bound LPL could hydrolyze interstitial lipids and thereby supply oligodendrocytes with fatty acid nutrients.
    Keywords:  GPIHBP1; lipoprotein lipase; myelin; oligodendrocyte
    DOI:  https://doi.org/10.1073/pnas.2610646123
  16. Cell Metab. 2026 Jun 02. pii: S1550-4131(26)00184-1. [Epub ahead of print]38(6): 1089-1092
      Mitochondria are classically viewed as a uniform ATP-producing network; however, a growing body of evidence suggests distinct subpopulations exist within tissues and even single cells. Here, I highlight evidence supporting the presence of functionally distinct mitochondria and propose mechanisms by which these subpopulations are formed and regulated.
    DOI:  https://doi.org/10.1016/j.cmet.2026.04.019
  17. bioRxiv. 2026 May 21. pii: 2026.05.20.726454. [Epub ahead of print]
      Astrocytes are key regulators of lipid metabolism, and dysregulated astrocytic lipid processing is implicated in Parkinson's disease (PD) pathogenesis. Our prior genome-wide screens identified ACSBG1, an astrocyte-enriched acyl-CoA synthetase, as a candidate regulator of α-synuclein (α-Syn) levels. However, how ACSBG1 links lipid reprogramming to inflammatory astrocyte activation and α-Syn pathology remains unknown. We compared the transcriptomic, cytokine, and lipid secretomes of TNF-α and IL-1α stimulated primary astrocytes from wild-type (WT) and Acsbg1 knockout (KO) mice. In vivo, we crossed Acsbg1 KO mice with a Thy1-α-Syn PD model to assess behavior, neuroinflammation, synaptic integrity, and α-Syn levels. Following cytokine exposure, Acsbg1 KO astrocytes mounted an attenuated inflammatory transcriptional response, secreting significantly fewer inflammatory mediators (e.g., IL-6, RANTES, MIP-3α) and less long-chain Sphingosine 20:1 than WT astrocytes. Importantly, exogenous Sphingosine 20:1 or cytokines from WT reactive astrocytes induced neuronal α-Syn phosphorylation (pS129). In vivo, Acsbg1 deletion in Thy1-α-Syn mice reduced astrogliosis, rescued synaptic and behavioral deficits, and decreased total and pS129-α-Syn. These findings establish ACSBG1 as a key regulator of inflammatory astrocyte signaling that contributes to α-Syn phosphorylation via specific cytokine and lipid mediators, identifying ACSBG1 as a novel therapeutic target for modulating astrocyte-neuron communication in PD.
    DOI:  https://doi.org/10.64898/2026.05.20.726454
  18. Biochemistry (Mosc). 2026 May;91(5): 770-779
      Modern magnetic resonance imaging (MRI) methods enable individualized assessment of both functional brain activity and neurochemical composition. Functional magnetic resonance imaging (fMRI) allows evaluation of brain activity at rest and during task performance, while magnetic resonance spectroscopy (MRS) provides measurements of key metabolites such as choline, N-acetylaspartate, creatine, lactate, lipids, alanine, glutamine and glutamate, GABA, and myo-inositol. These approaches are widely used in both fundamental brain research and diagnostic studies. However, existing literature lacks methods for directly comparing these individual assessments, which is essential for investigating relationships between metabolite levels and brain activity. Here, we present a method for aligning individual fMRI and MRS data. Using this approach, we demonstrated a neurophysiological phenomenon in which the functional connectivity between brain regions increases while overall functional activity decreases during task performance.
    Keywords:  BOLD signal; connectomics; fMRI; functional connectivity; glutamate; magnetic resonance spectroscopy
    DOI:  https://doi.org/10.1134/S0006297926600213
  19. ACS Chem Neurosci. 2026 Jun 02.
      Neurodegenerative disorders increasingly reflect failures of cellular state control rather than the linear accumulation of a single toxic lesion. Microglia become trapped in maladaptive states in which inflammatory activation is decoupled from effective cargo processing. Lipid droplet-accumulating microglia (LDAM) represent a recurrent convergence state across aging and neurodegeneration, characterized by persistent neutral lipid sequestration, reduced phagocytosis-to-degradation capacity, oxidative amplification, and chronic but functionally inefficient inflammation. LDAM emerges when lipid substrate influx exceeds the capacity of cholesterol efflux, lysosomal lipophagy, and mitochondrial β-oxidation, converting lipid droplets from transient buffers into stable metabolic anchors. This entrenchment is reinforced by mitochondrial exhaustion, vacuolar H+-ATPase-linked lysosomal deacidification, and inflammasome/interferon locking, often further amplified by cGAS-STING signaling. Together, these constraints converge on a state of metabolic-epigenetic locking that sustains permissive chromatin landscapes at pro-inflammatory loci. On this basis, state-resetting strategies are considered that rebalance lipid flux, restore organelle clearance capacity, and transiently restrain inflammatory amplification, while spatial multiomics and fluid biomarkers are discussed as candidate tools for stage- and niche-resolved stratification of combination interventions.
    Keywords:  lipid droplet-accumulating microglia; lipid flux; lysosomal lipophagy; trained immunity
    DOI:  https://doi.org/10.1021/acschemneuro.6c00284
  20. Free Radic Biol Med. 2026 May 29. pii: S0891-5849(26)00839-7. [Epub ahead of print]253 377-392
      Brain endothelial cells (BECs) form the structural foundation of the blood-brain barrier (BBB) and exhibit a paradoxical metabolic phenotype, converting approximately 90% of consumed glucose to lactate despite residing in an oxygen-rich vascular environment. Whether the extracellular lactate that BECs continuously produce and export feeds back to regulate their own metabolism and redox state has not been directly investigated. Here, using validated BBB model, we demonstrate that exogenous lactate drives a concentration- and time-dependent biphasic growth response, with 10 mM lactate maximally promoting BEC proliferation at 48 h. Mechanistically, lactate suppresses canonical glycolysis evidenced by downregulation of GLUT1 and key glycolytic enzymes and reduced glucose uptake while simultaneously driving a coordinated shift toward mitochondrial oxidative metabolism. This shift is mediated by upregulation of LDHB, MPC1, MPC2, and PDH activation, enabling lactate-derived pyruvate to enter mitochondria and fuel comprehensive TCA cycle engagement, mitochondrial biogenesis, and enhanced oxidative phosphorylation capacity as measured by high-resolution respirometry. At the redox level, lactate oxidation imposes reductive pressure on the NAD+/NADH pool, which is counterbalanced by activation of the NAMPT-dependent NAD+ salvage pathway, resulting in expansion of the total NAD pool. Genetic silencing of LDHB or MPC1 and pharmacological inhibition of NAMPT each independently abolish lactate-driven BEC proliferation, establishing the LDHB-MPC-NAD+ axis as mechanistically essential. These findings identify the cerebrovascular endothelium as an active participant in brain lactate mitochondrial function and introduce the LDHB-MPC-NAD+ axis as a novel redox-metabolic regulatory circuit at the BBB.
    DOI:  https://doi.org/10.1016/j.freeradbiomed.2026.05.335
  21. Cell Mol Life Sci. 2026 Jun 06.
      OXCT1 (3-oxoacid CoA-transferase 1), encoding the mitochondrial enzyme SCOT (succinyl-CoA:3-ketoacid CoA transferase), is a key enzyme in ketone body utilization. It catalyzes the reversible transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA, which is subsequently converted into acetyl-CoA for entry into the tricarboxylic acid (TCA) cycle and mitochondrial energy production. Although classically regarded as a metabolic enzyme, emerging evidence indicates that OXCT1 participates in broader regulatory networks. In addition to its protein-coding transcript, the OXCT1 locus produces regulatory non-coding RNAs, including circ-OXCT1 and lncRNA OXCT1-AS1, which provides additional levels of regulation, as for now reported in various types of cancer. Moreover, the review summarizes current knowledge on OXCT1 biochemical function, regulation, and tissue distribution, with emphasis on transcriptional control, post-translational modifications such as lysine succinylation and redox-dependent regulation, and integration with nutrient-sensing and stress-response pathways. By combining recent literature with bioinformatics analysis, we demonstrate that OXCT1 displays highly dynamic expression across diverse cancer types and metabolic states, consistent with a role in metabolic plasticity and disease progression. Furthermore, we discuss here the OXCT1 role in other pathological conditions including metabolic disorders, neurological disease, and cardiomyopathy, implicating both metabolic dysfunction and aberrant protein modification in disease mechanisms. Collectively, these findings establish OXCT1 as a central regulator of metabolic adaptation and a potential therapeutic target.
    Keywords:  Cancer; Ketogenic diet; Ketone bodies; OXCT1; Succinylation; β-hydroxybutyrate
    DOI:  https://doi.org/10.1007/s00018-026-06264-4
  22. J Cereb Blood Flow Metab. 2026 May 31. 271678X261458133
      Ischemic stroke (IS) causes white matter (WM) injury and disrupts myelin integrity, contributing to long-term cognitive dysfunction. Oligodendrocyte lineage cells, particularly oligodendrocyte precursor cells (OPCs), are essential for post-stroke remyelination, yet their survival, differentiation, and myelin-forming capacity are highly dependent on metabolic state. However, a systematic overview of these metabolic changes post-stroke is still lacking. Here, we review current knowledge on the crosstalk between classical pathways and oligodendrocyte lineage metabolism in IS, integrating evidence from recent studies and complementing them with a re-analysis of three independent single-cell RNA sequencing (scRNA-seq) datasets from mouse stroke models covering hyperacute, acute, and chronic recovery phases. This re-analysis highlighted stage-specific alterations in oxidative phosphorylation, inositol phosphate metabolism, and sphingolipid metabolism within OPCs, alongside activation of cholesterol biosynthesis in mature oligodendrocytes (OLs). These findings are consistent with evidence linking these pathways to oligodendrocyte lineage progression and WM repair. Together, the literature and re-analysis support the notion that oligodendrocyte lineage metabolism is an important regulator of post-stroke remyelination and may provide potential therapeutic targets for promoting cognitive function.
    DOI:  https://doi.org/10.1177/0271678X261458133
  23. Neurochem Int. 2026 Jun 04. pii: S0197-0186(26)00084-7. [Epub ahead of print] 106193
      Cerebral ischemia-reperfusion injury (CIRI) frequently compromises neurological outcomes in stroke patients despite successful recanalization therapy. While the brain's dense lipid network is integral to immune regulation and tissue repair, the spatiotemporal disruption of lipid metabolism during CIRI remains incompletely synthesized. This review critically evaluates the dynamic alterations in brain lipid profiles following ischemic injury, with a specific focus on the microglial niche. We detail how the excessive influx of extracellular lipid debris forces microglia into maladaptive, lipid-droplet-accumulating states, thereby amplifying lipotoxicity, the neuroinflammatory storm, and microglial vulnerability to ferroptosis. By integrating current evidence on lipid-driven microglial dysfunction, this review highlights the "lipid-inflammation-ferroptosis" axis as a contributing pathogenic mechanism in CIRI. Finally, we discuss the translational potential and current limitations of targeting microglial lipid metabolism, offering a balanced perspective on developing targeted metabolic interventions to enhance stroke recovery.
    Keywords:  brain ischemia; ferroptosis; inflammation; lipid metabolism; microglia
    DOI:  https://doi.org/10.1016/j.neuint.2026.106193
  24. Front Aging Neurosci. 2026 ;18 1843643
      Oligodendrocyte lineage cells, consisting of mature oligodendrocytes (mOLs) and their progenitors (OPCs), sustain myelination and support axonal metabolic integrity in the mammalian central nervous system. In neurodegenerative disorders, functional deficits spanning impaired mOL homeostasis and dysregulated OPC activation are no longer regarded as passive secondary outcomes of neuronal injury. Instead, emerging clinical and preclinical data demonstrate that oligodendroglial dysfunction actively fuels disease progression. Notably, most human evidence remains correlative, with few definitive proofs that OL pathology initiates neurodegeneration, indicating lineage malfunction predominantly exacerbates, rather than triggers, disease onset across Alzheimer's, Parkinson's, and Huntington's diseases. In this review, we synthesize disease-specific oligodendrocyte pathological signatures and context-dependent cellular responses, focusing on underrecognized OL-intrinsic pathogenic mechanisms: endogenous Aβ production, aberrant protein aggregation, disrupted cholesterol turnover, and excessive neuroinflammatory amplification. We further establish a unified mechanistic model to explain the widespread heterogeneity of white matter pathology across distinct neurodegenerative contexts. We detail four core interconnected pathways whereby defective OL lineage function drives tissue deterioration: myelin loss and progressive axonal degeneration, disrupted neuroimmune homeostasis, cytotoxicity from aggregated pathological proteins, and dysregulated metabolic signaling. To resolve persistent conceptual confusion in the field, we strictly distinguish cell-autonomous primary oligodendroglial lesions, secondary reactive changes following neuronal damage, and non-specific white matter remodeling. We also address critical translational barriers stemming from well-documented phenotypic discrepancies between animal models and human patient brains. Moreover, we consolidate current OL-targeted therapeutic strategies, including myelin restoration, immunomodulatory intervention, metabolic reprogramming, and gene-targeted therapy, highlighting the clinical bottlenecks of single-target regimens and the superior translational prospects of multi-target combinatorial strategies. We conclude by outlining key unresolved challenges and future research avenues, covering OL subtype identification, intercellular signaling crosstalk characterization, humanized model optimization, and precision delivery technique innovation. Collectively, this review refines our understanding of context-dependent oligodendrocyte biofunctions in neurodegeneration, clarifies the origin and consequence of white matter lesions, and offers actionable mechanistic and theoretical support for developing novel glia-based clinical therapies.
    Keywords:  metabolic disorder; myelin damage; neurodegenerative diseases; oligodendrocytes; targeted therapy
    DOI:  https://doi.org/10.3389/fnagi.2026.1843643
  25. J Anat. 2026 Jun 04.
      In our companion paper, in this issue, we describe our efforts to provide embryonic rhesus macaque (Macaca mulatta) brain histology for the study of brain development, with emphasis on cortical development. Here we continue our description of efforts to provide postnatal rhesus macaque brain histology relevant for the study of cellular circuits which continue to mature and change well into postnatal life, in males and females, and which naturally deteriorate in the elderly. The mission of the MacBrain Resource Center (MBRC) in the Department of Neuroscience at Yale University School of Medicine is to provide a cost-effective means for researchers to conduct de novo studies on this non-human primate (NHP) brain animal model using materials already in existence and therefore without exorbitant costs and without having to sacrifice additional animals (https://medicine.yale.edu/neuroscience/macbrain/mission/). Here we report on how this mission is being accomplished. Because MBRC materials have been and continue to be gathered from unrelated studies over many years, most methods have already been published. The MBRC divides different types of materials into separate Collections. The present description of histo- and immunohistochemical processes is limited to current work that provides materials to populate Collection 6 and is accurate as of May 2026. Collections in the MBRC are dynamic. Of the 8 current MBRC datasets, here we emphasize Collections 5, 6, and 7 and illustrate through examples how different materials are currently being used to conduct research both in cortical and subcortical structures. Many of the electron microscopy (EM) blocks in Collection 5 sampling the brain at numerous regions come from the >100 cases of titrated thymidine (3H-TdR) injections in Collection 1 in addition to cases in Collections 2 and 3. Altogether, at present there are ~1000 inventoried EM blocks collected from postnatal cases. Collection 6 currently contains >30,000 digital images illustrating 35 different cellular and fiber markers in 32 brains of both sexes ranging from P0 to 32 years of age. Materials in Collections 6 and 7 keep growing as we constantly process and add NHP brains to them. Based on the molecular, genetic, and anatomical similarities between this animal model and human, we underline the importance of archiving and (re-)using rhesus macaque brains to foster neuroscience research. As far as we know the NHP brain materials in the MBRC Collections constitute the largest datasets of their kind in the world.
    Keywords:   macaca mulatta ; autoradiography; databases; electron; factual; immunohistochemistry; microscopy; tissue banks
    DOI:  https://doi.org/10.1111/joa.70183
  26. Transl Psychiatry. 2026 Jun 02.
      Recent research highlights the critical role of white matter (WM) alterations in psychosis and schizophrenia (SZ), reporting volumetric and structural brain changes in affected individuals. In this study, we explored the role of astroglia in SZ, which is believed to play a role in white matter integrity. We investigated for the first time the associations between advanced diffusion Magnetic Resonance Imaging (dMRI) measures of WM microstructure and Magnetic Resonance Spectroscopy (MRS)-derived glial markers in 30 subjects with early psychosis (EP, mean age 24 ± 6) versus 49 healthy controls (HC, mean age 25 ± 6). We focused on two metabolites involved in glia: myo-Inositol (myo-Ins) and total Choline (tCho), measured in the medial prefrontal cortex (mPFC), relating them to quantitative dMRI metrics derived from Diffusion Kurtosis Imaging (DKI) and WM Tract Integrity-Watson (WMTI-W) biophysical model, including mean diffusivity and kurtosis, axonal water fraction and extra-axonal diffusivities in the whole white matter. Our findings reveal a difference between EP and HC in WM diffusivities, specifically in the extra-axonal parallel direction, but not in MRS metabolites. However, we found that the mPFC myo-Ins concentrations in EP are exclusively and strongly associated with proximal WM microstructure features, in the form of a positive correlation with axonal water fraction, a proxy for axonal density, and a negative correlation with extra-axonal parallel diffusivity, suggesting the white matter alterations could be linked to astrocytic changes in early psychosis.
    DOI:  https://doi.org/10.1038/s41398-026-04072-9
  27. Sci Rep. 2026 May 30.
      Preterm infants exhibit metabolic immaturity, yet metabolic heterogeneity within this population remains underexplored. We performed targeted metabolomics on dried blood spots from 448 preterm (32-36 weeks) and 351 term neonates (37-40 weeks of gestation) using tandem mass spectrometry. Compared with term infants, preterm neonates showed significantly elevated tyrosine, leucine/isoleucine, arginine, and hydroxyoctadecenoylcarnitine (C18:1-OH), along with reduced glutamate (false discovery rate < 0.05). Multivariate analyses, including principal component analysis and partial least squares-discriminant analysis, identified three distinct metabolic clusters associated with gestational maturity and redox-related pathway signals. Pathway enrichment analysis highlighted disruptions in the urea cycle, ammonia recycling, purine metabolism, and mitochondrial fatty acid oxidation. Notably, C18:1-OH emerged as a key discriminatory metabolite and a potential biomarker of mitochondrial immaturity and altered fatty acid oxidation in preterm neonates. These findings support the presence of metabolically distinct subtypes within preterm infants and suggest that metabolomic profiling may contribute to precision neonatal risk stratification, although longitudinal validation is required.
    Keywords:  Biomarker; Metabolic subtypes; Metabolomics; Preterm neonates; Tandem mass spectrometry
    DOI:  https://doi.org/10.1038/s41598-026-50955-8
  28. Sci Rep. 2026 Jun 05.
      Adult hippocampal neurogenesis is a metabolically demanding process requiring tight coordination between energy production and biosynthetic flux. Although voluntary running is a potent stimulus for this plasticity, the metabolic landscape sustaining the neurogenic niche remains incompletely defined. Using untargeted gas chromatography/mass spectrometry-based metabolomics to characterize the hippocampal metabolome of mice following eight weeks of voluntary running, we identified metabolic changes consistent with coordinated metabolic reprogramming that suggest an adaptive metabolic stress response. A significant catabolic shift, marked by depletion of glutamic and aspartic acids, is associated with increased bioenergetic utilization and possible integration of neurotransmitter-derived substrates into central carbon metabolism. The exercise-induced elevation of CoA-related metabolites and tricarboxylic acid cycle intermediates is indicative of increased mitochondrial bioenergetic demand. Simultaneously, elevated nitrogenous metabolites, such as asparagine and glycine, coincide with increased availability of biosynthetic precursors for nucleotide synthesis, redox balance, and structural remodeling linked to neurogenesis. Enrichment of one-carbon metabolism is compatible with integration of metabolic pathways involved in biosynthetic and regulatory processes related to neurogenic remodeling. Together, these findings align with the interpretation that voluntary running may act as a metabolic hormetic stimulus, linked to reconfiguration of hippocampal metabolic networks to support a permissive environment for neurogenic plasticity and cognitive resilience.
    DOI:  https://doi.org/10.1038/s41598-026-54888-0