bims-medebr Biomed News
on Metabolism of the developing brain
Issue of 2026–02–15
twenty-one papers selected by
Regina F. Fernández, Johns Hopkins University



  1. bioRxiv. 2026 Feb 07. pii: 2026.02.07.704575. [Epub ahead of print]
      Niemann - Pick Disease Type C1 (NPC1) is a fatal, neurodegenerative disorder, characterized by lysosomal lipid accumulation and dysmyelination. Previous studies have documented some lipid abnormalities in the null mouse focused on the whole brain and liver. However, the specific lipidomic alterations in severely affected brain regions, such as cerebellum and isolated myelin remain understudied. We present a comprehensive LC - MS - based lipidomic analysis of the cerebellum and cortex of Npc1-/- mice during disease progression stages, along with the first comprehensive characterization of the myelin lipidome in NPC1 disease. Our results reveal that the cerebellum accumulates lipid species, including sphingolipids and glycerophospholipids progressively, while the cortex shows an overall decline in lipid levels, indicating region-specific lipid dysregulation. Notably, bis(monoacylglycero)phosphates and their precursors including lysophosphatidylglycerol and hemibismonoacylglycerophosphate exhibit significant accumulation, with a preference for docosahexaenoic acid (DHA) containing species. Despite known cholesterol storage defects in NPC1, we observed reduced free cholesterol levels in both regions, which we attribute to myelin loss. Myelin-specific lipidomics demonstrated extensive dysregulation, particularly in cortical myelin, including severe losses in sulfatides, ether-lipids, and acylcarnitine, alongside striking accumulation of hydroxy-ceramides. These findings identify novel lipid alterations in brain subregions and myelin, offering critical insight into the lipid perturbations under the loss of NPC1, and highlight lipid targets that may be crucial for therapeutic intervention and biomarker development.
    DOI:  https://doi.org/10.64898/2026.02.07.704575
  2. Autophagy. 2026 Feb 12. 1-3
      Mitochondria regulate ATP production, calcium buffering, and apoptotic signaling, and clearing dysfunctional mitochondria by mitophagy is essential for cellular homeostasis. While PINK1-dependent mitophagy is well-characterized in neurons, its function in glial cells such as astrocytes is less understood. Our study demonstrates that PINK1-mitophagy in astrocytes occurs faster and with less spatial restriction compared to neurons. This pathway was specifically regulated in astrocytes by the glycolytic enzyme, HK2 (hexokinase 2), which forms a glucose-dependent complex with PINK1 following mitochondrial damage. Inflammation also induces HK2-PINK1 mitophagy, and its activation in astrocytes protects against cytokine-induced neuronal death. Our findings characterize a novel HK2-PINK1 pathway in astrocytes that bridges mitophagy, metabolism, and immune signaling.Abbreviation: HK2: hexokinase 2; PD: Parkinson disease; PINK1: PTEN induced kinase 1; S65: serine 65.
    Keywords:  Astrocyte; HK1; PINK1; mitochondria; mitophagy; neurodegeneration; parkin
    DOI:  https://doi.org/10.1080/15548627.2026.2623987
  3. NPJ Metab Health Dis. 2026 Feb 10. 4(1): 7
      Developing cells undergo extensive metabolic adaptations to support growth and differentiation. Here, using spatially resolved mass spectrometry imaging and stable isotope tracing, we systematically investigate metabolic remodeling in mouse brains at postnatal day 14 and day 28, a period coinciding with the transition from a maternal milk diet to solid food. Untargeted metabolomics reveals global shifts in lipid composition, and region-specific remodeling of central energy metabolism, including increased glycolytic intermediates in grey matter-enriched regions and a global decrease in tricarboxylic acid (TCA) cycle metabolites after weaning. Despite these marked changes in metabolite levels, the glucose incorporation rate remains constant across these developmental stages. Notably, weaning mice onto a milk-replacement diet demonstrates that the observed metabolic adaptations are largely diet-independent. Together, our data suggest that postnatal brain metabolic remodeling is an intrinsically programmed feature of maturation providing region-specific metabolic reorganization to support developmental demands.
    DOI:  https://doi.org/10.1038/s44324-025-00098-7
  4. J Cereb Blood Flow Metab. 2026 Feb;46(2): 493-507
      Ischemic stroke remains a major contributor to neurological disability worldwide, with limited options for promoting brain recovery. While acetate is known to exert various pathophysiological effects on the brain, its potential to mitigate brain injury following ischemic stroke and the underlying mechanisms remain poorly understood. In particular, how acetate engage astrocyte-neuron metabolic coupling and influence tau protein modifications remains to be clarified. Here, we found that brain acetate levels were elevated by 1.51-fold after ischemia and reperfusion for 24 h. The accumulated acetate in the brain improved survival with a 7-day survival rate of 61% in the acetate group versus 47% in the control group and attenuated brain injury. Mechanistically, acetate stimulated the astrocyte-neuron lactate shuttle (ANLS), leading to increased tau protein lactylation in neurons. This process was blocked by Su3118, a pharmacological inhibitor of monocarboxylate transporter 1/4 (MCT1/4), confirming their involvement. We further revealed that site-specific tau lactylation enhanced neuronal plasticity and rendered the brain less sensitive to ischemic stroke. Taken together, our findings unveil that acetate enhances astrocytic glucose uptake and glycolysis, stimulates ANLS, and promotes tau protein lactylation in neurons, thereby contributing to its neuroprotective role, highlighting its relevance for developing acetate-based interventions in stroke recovery.
    Keywords:  Acetate; astrocyte-neuron lactate shuttle (ANLS); ischemic stroke; protein lactylation; tau protein
    DOI:  https://doi.org/10.1177/0271678X251385091
  5. Prog Lipid Res. 2026 Feb 07. pii: S0163-7827(26)00003-2. [Epub ahead of print]101 101378
      In the last few years, fatty acids have gained increasing recognition as key modulators of neurodegenerative disease onset and progression. As the fundamental building blocks of most lipids, they not only maintain membrane structure but also support diverse cellular functions. In this review, we summarize their biochemical structures and major classifications, and we describe the pathways governing their synthesis, uptake, and trafficking in the brain. We further explore how fatty acids influence electrophysiological processes by modulating membrane channel conductance, ion-channel-gating, and receptor-mediated signaling, thereby impacting synaptic transmission and cognition performance. Finally, we examine evidence linking fatty acid dynamics in neurodegenerative pathophysiology, highlighting their dual role as both protective and detrimental agents in brain health.
    Keywords:  Cognition; Diet; Electrophysiological properties; Fatty acids; Neurodegenerative diseases; Transport
    DOI:  https://doi.org/10.1016/j.plipres.2026.101378
  6. Cell Tissue Res. 2026 Feb 02. 403(2): 15
      Neu1 (lysosomal sialidase 1) is essential for removing sialic acid from oligosaccharides and glycoconjugates. Neu1 deficiency impairs lysosomal digestion, leading to sialidosis and sialoglycoprotein accumulation. It also increases lipids, including gangliosides GM3, GD3, GM4, and LM1, in the kidney, liver, and spleen. Neu1-/- mice display symptoms resembling Type II sialidosis, including enlarged spleen and liver, kidney issues, neurological problems, spinal defects, and oligosaccharide buildup. The study examined secondary lipid alterations and inflammation in the cortex and cerebellum of these mice. Lipidomic, molecular, and immunohistochemical analyses of tissues from 2 and 5 M Neu1-/- mice revealed reduced levels of lipids, including PC, PE, PS, and CL, along with increased pro-inflammatory cytokines and loss of oligodendrocytes and neurons. Signs of astrogliosis and microgliosis emerged in specific brain regions. These results indicate that reduced levels of glycerophospholipids could serve as an indicator of inflammation in sialidosis mice. Future research should investigate therapies targeting these lipid changes, as modulating glycerophospholipids might slow disease progression in sialidosis patients.
    Keywords:  Mouse model; Neu1 sialidase; Neuroinflammation; Secondary lipids
    DOI:  https://doi.org/10.1007/s00441-026-04045-w
  7. Brain Behav Immun Health. 2026 Mar;52 101178
      Neuroinflammation is increasingly implicated in post-infectious brain atrophy and cognitive impairment, as well as the etiology of neurodegenerative and psychiatric disorders. Here, we employed an established mouse model of neuroinflammation (systemic lipopolysaccharide [LPS]) and studied its macrostructural, microstructural, and cellular effects on the mouse brain. We combined whole-brain two-photon microscopy, multiparametric magnetic resonance imaging, and in silico transcriptomics. We demonstrate that LPS increases microglial soma size, a marker of microglial proinflammatory transformation, throughout the brain. LPS also induces in vivo volumetric increases of multiple brain regions, but not of the cerebral cortex. These in vivo increases are no longer observed ex vivo (post-fixation), suggesting they may be due to brain edema. Moreover, LPS induces microstructural alterations, as indicated by ex vivo reductions in fractional anisotropy and magnetization transfer ratio, which were most pronounced in the cerebral cortex. These reductions had the largest effect size in regions with higher expression of genes specific to parvalbumin inhibitory neurons and excitatory layer 4/5 intratelencephalic neurons, which were enriched in genes encoding synapse components. Thus, these results suggest that LPS-induced neuroinflammation may lead to brain edema, which spares the cerebral cortex, and microstructural alterations, which impact the cerebral cortex and may involve specific subtypes of inhibitory and excitatory neurons and their synapses.
    Keywords:  Bioinformatics; Cellular component of synapse; In silico transcriptomics; Layer 4,5 excitatory neuron; Neuroinflammation; PVALB inhibitory neuron; Two-photon imaging of microglia; multimodal magnetic resonance imaging of the brain
    DOI:  https://doi.org/10.1016/j.bbih.2026.101178
  8. Adv Sci (Weinh). 2026 Feb 12. e19874
      Postoperative cognitive dysfunction (POCD) negatively impacts prognosis; however, the underlying mechanisms remain unclear. We demonstrated that tibial fracture surgery led to cognitive dysfunction in 18-month-old mice, concomitant with a reduction in hippocampal levels of cholesterol and its key metabolite 24-hydroxycholesterol (24-OHC). Clinically, reduced blood 24-OHC levels were associated with cognitive decline in elderly surgery patients. Mechanistically, downregulation of sterol regulatory element-binding protein 2 (SREBP2) in reactive astrocytes of the hippocampal dorsal CA1 (dCA1) region was an important cause of postoperative cholesterol deficiency, which in turn impaired synaptic plasticity and excitatory synaptic transmission; furthermore, this deficit could be rescued by direct cholesterol replenishment in the dCA1. Importantly, we established multiple effective therapeutic strategies-astrocyte-specific SREBP2 overexpression, chemogenetic suppression of reactive astrocytes, and minocycline administration-all of which effectively reversed surgery-induced cholesterol loss, alleviated synaptic dysfunction, and ultimately improved cognitive performance. Taken together, our findings not only position astrocytic SREBP2 as a promising therapeutic target for POCD but also highlight the potential diagnostic value of monitoring brain cholesterol metabolism, though this requires validation in larger longitudinal cohorts.
    Keywords:  SREBP2; excitatory synaptic transmission; neuroinflammation; postoperative cognitive dysfunction; reactive astrocyte; synaptic plasticity
    DOI:  https://doi.org/10.1002/advs.202519874
  9. J Neurochem. 2026 Feb;170(2): e70378
      Glutathione is a major component of the cellular antioxidant system, providing a means of controlling redox homeostasis and affording protection against oxidative damage. Proton magnetic resonance spectroscopy (MRS) offers insights into brain metabolism by enabling the noninvasive quantification of metabolites. Previous studies have demonstrated that the neurotransmitters glutamate and GABA detected by MRS show activity-dependent concentration changes and correlate with cognitive performance. Yet how MRS detected antioxidant capacity, particularly glutathione levels, relates to cognition remains unclear. In this issue, Lee et al. report that higher cortical glutathione levels are associated with better cognitive outcomes in older adults. These findings might contribute to understanding whether glutathione levels index resilience or degeneration. However, observations reported across the literature remain inconsistent, and the observed discrepancies underscore the need for further research using harmonized MRS acquisitions, deeper metabolic and cognitive phenotyping, and longitudinal study designs to clarify the role of cortical glutathione in cognitive trajectories.
    Keywords:  cognition; glutathione; magnetic resonance spectroscopy; metabolism
    DOI:  https://doi.org/10.1111/jnc.70378
  10. Front Aging Neurosci. 2026 ;18 1713583
      Lactate is the terminal product of anaerobic oxidation within the glucose metabolism pathway. Traditionally, lactate has been regarded as a metabolically insignificant byproduct derived from incomplete oxidation. However, recent evidence suggests that lactate plays dual roles in the nervous system: neuroprotective and neurotoxicity. The diverse functions of lactate in the nervous system are influenced by its varying concentrations and distinct signal transduction pathways. This review focuses on elucidating the molecular mechanisms underlying lactate's functions through energy metabolism, neurodegeneration, neural excitation, and neuroinflammation, particularly the signaling pathways involved in neuroprotection and neuroinjury. Furthermore, we highlight several pharmacological agents associated with these processes, aiming to provide novel insights and therapeutic strategies for neuroprotection under specific conditions such as hypoxia, and the management of neurological disorders.
    Keywords:  lactate; lactylation modification; nervous system; neuroinjury; neuroprotection
    DOI:  https://doi.org/10.3389/fnagi.2026.1713583
  11. Mol Neurodegener Adv. 2026 ;2(1): 11
       Background: Alzheimer's disease (AD) is molecularly heterogeneous. In our previous cerebrospinal fluid (CSF) proteomic study in AD, we identified and validated five distinct molecular subtypes characterized by neuronal hyperplasticity (subtype 1), innate immune activation (subtype 2), RNA dysregulation (subtype 3), choroid plexus dysfunction (subtype 4) and blood-brain barrier impairment (subtype 5). These subtypes also differed in the CSF levels of proteins involved in lipid metabolism, suggesting that lipid dysregulation in AD might be subtype specific.
    Methods: We performed untargeted lipidomics on CSF samples from 601 individuals in the Amsterdam Dementia Cohort who were previously included in our proteomic study (n = 416 AD, 185 controls). Using the CSH-QTOF platform for complex lipids, 3,532 lipids were detected in CSF, 270 of which could be mapped to 13 different lipid classes. Lipid levels were compared between each AD subtype and controls using linear regression models adjusted for age and sex (R v4.2.1). Lipids with significantly different levels (p < 0.05) were included for pathway enrichment analysis with MetaboAnalyst6.0.
    Results: We observed alterations in the levels of 1,893 lipids, with the majority associated with a single AD subtype. Subtype 3 (RNA dysregulation) exhibited the most pronounced alterations, with altered CSF levels of 669 lipids, including triglycerides and fatty acids, which were reduced compared to controls. Subtype 4 (choroid plexus dysfunction) and subtype 5 (blood-brain barrier dysfunction) both had alterations in the same set of 150 lipids, but with changes occurring in opposite directions (i.e., decreased in subtype 4, and increased in subtype 5). These lipids were associated with sphingolipid metabolism and lipid transport. Subtype 1 (neuronal hyperplasticity) and subtype 2 (innate immune activation) had less pronounced differences compared to the other subtypes. Subtype 1 had increased levels of several phospholipids, indicating neuronal membrane remodeling, and subtype 2 decreased arachidonic acid levels, a precursor of immunoregulatory oxylipins.
    Conclusion: Our findings reveal subtype-specific lipid metabolism alterations in AD. Currently, five lipid-targeting drugs are in phase 1 and 2 trials. Our results suggest that treatment efficacy may vary by subtype. Understanding these molecular differences can inform trial design and analysis, advancing the development of tailored therapies for AD.
    Supplementary Information: The online version contains supplementary material available at 10.1186/s44477-026-00018-z.
    Keywords:  Alzheimer’s disease; Heterogeneity; Lipid metabolism; Lipidomics; Molecular subtypes
    DOI:  https://doi.org/10.1186/s44477-026-00018-z
  12. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2025 Nov 28. pii: 1672-7347(2025)11-2124-09. [Epub ahead of print]50(11): 2124-2132
      The metabolism of lipids such as fatty acids, cholesterol, and phospholipids in the brain plays a critical role in maintaining neuronal membrane structure, signal transduction, and neurotransmitter regulation. In recent years, studies have found a close association between dysregulated lipid metabolism and the development of depression. Disruption of lipid metabolism may affect the function of neural networks, leading to impaired emotional regulation and an increased risk of depression. Therefore, an in-depth exploration of the relationship between lipids and depression is of great significance for elucidating the biological basis and pathogenesis of depression. This review systematically summarizes dysregulation of 3 categories of lipid metabolism, fatty acids, cholesterol, and phospholipids, and their potential mechanisms in influencing the onset of depression through pathways such as regulation of neurotransmitters, mediation of inflammatory responses, and involvement in oxidative stress pathways. It also summarizes 4 categories of existing antidepressant treatment strategies related to brain lipid metabolism, chemical drugs, dietary interventions, traditional Chinese medicine compound formulas, and natural drugs, with the aim of clarifying the potential application value of brain lipid metabolism in the prevention and treatment of depression and providing a scientific basis for future translational directions and clinical practice in this field.
    Keywords:  antidepressant drugs; central nervous system; depression; lipid metabolism; mechanisms
    DOI:  https://doi.org/10.11817/j.issn.1672-7347.2025.250401
  13. Sci Transl Med. 2026 Feb 11. 18(836): eadw0834
      Hyperactivation of glucose metabolism to lactate is a metabolic hallmark of cancer. However, the functional role of lactate in pediatric diffuse midline glioma (DMG) cells is unclear. Here, using stable isotope tracing and loss-of-function studies in clinically relevant patient-derived DMG models, we show that the oncogenic histone H3K27M mutation epigenetically up-regulates the rate-limiting glycolytic enzyme phosphoglycerate kinase 1 (PGK1) and drives lactate production from [U-13C]-glucose in DMGs. Mechanistically, lactate posttranslationally activates the nucleoside diphosphate kinase NME1 through lactylation and facilitates the synthesis of nucleoside triphosphates that are essential for DNA replication and tumor proliferation. This mechanistic link between glycolysis and nucleotide biosynthesis provides the opportunity for deuterium metabolic imaging of tumor growth and response to therapy. Spatially mapping 2H-lactate production from [6,6-2H]-glucose allows visualization of the metabolically active tumor lesion and provides an early readout of response to standard of care and targeted therapy that precedes extended survival and reflects pharmacodynamic alterations in tumor tissues in preclinical DMG models in vivo at clinical field strength (3 T). Overall, we have identified an H3K27M-lactate-NME1 axis that drives DMG proliferation and facilitates noninvasive in vivo metabolic imaging of DMGs.
    DOI:  https://doi.org/10.1126/scitranslmed.adw0834
  14. bioRxiv. 2026 Feb 04. pii: 2026.02.02.703345. [Epub ahead of print]
      Mitochondria and inflammation are tightly linked in aging and Alzheimer's disease (AD), and recent evidence implicates mitochondrial double-stranded RNA (mt-dsRNA) as a potential trigger of inflammation. We examined mt-dsRNA accumulation and dsRNA signaling in brain aging and AD using human brain tissue and complementary in vitro transcriptomic datasets, quantifying mitochondrial transcripts and dsRNA editing. We found that mt-dsRNA accumulated after midlife and coincided with reduced expression of mitochondrial RNA processing and translation machinery, along with increased expression of dsRNA antiviral signaling proteins, consistent with cytoplasmic mt-dsRNA-driven inflammation. In AD brains, mt-dsRNA accumulation was further increased and correlated with cognitive impairment, neuropathological severity, and AD risk genotypes. Genes associated with these measures reflected altered ubiquitin-dependent regulation of antiviral signaling, potentially indicating altered sensitivity to mt-dsRNA. Together, these findings highlight mitochondrial RNA homeostasis as an unrecognized contributor to age- and AD-related neurodegeneration by identifying mt-dsRNA as a potential driver of chronic inflammation.
    Abstract Figure:
    DOI:  https://doi.org/10.64898/2026.02.02.703345
  15. Int J Mol Sci. 2026 Jan 29. pii: 1375. [Epub ahead of print]27(3):
      Biallelic variants in NDUFS6, encoding an accessory subunit of mitochondrial complex I, were initially associated with lethal neonatal mitochondrial encephalopathy and Leigh syndrome. Recent studies have demonstrated that NDUFS6 variants can also cause childhood- or adolescent-onset axonal neuropathy and Charcot-Marie-Tooth (CMT)-like phenotypes, indicating marked clinical heterogeneity. Here, we report a patient with a novel homozygous truncating NDUFS6 variant presenting with a neuropathy-predominant phenotype accompanied by epilepsy, in the absence of neonatal metabolic decompensation. The patient presented with childhood-onset progressive gait abnormality, pes cavus deformity, distal weakness requiring Achilles tendon-release surgery, pyramidal signs, urinary incontinence, and focal epileptiform EEG findings. Brain MRI showed bilateral lenticular nucleus abnormalities. Whole-exome sequencing identified a novel homozygous NDUFS6 nonsense variant (c.130C>T, p.Gln44*). While neuropathy has previously been reported primarily in association with the recurrent splice-site variant c.309+5G>A, our findings demonstrate that truncating NDUFS6 mutations can also underlie a neuropathy-predominant phenotype. Together with previously published cases, our findings support a phenotypic heterogeneity ranging from lethal encephalopathy to neuropathy and reinforce the role of NDUFS6 as a disease-causing gene for inherited peripheral neuropathy. These data support inclusion of NDUFS6 among established neuropathy and Charcot-Marie-Tooth genes.
    Keywords:  CMT; NDUFS6; epilepsy; neuropathy
    DOI:  https://doi.org/10.3390/ijms27031375
  16. bioRxiv. 2026 Feb 04. pii: 2026.02.02.703178. [Epub ahead of print]
      Aging is associated with widespread metabolic changes that contribute to functional decline and disease. While prior studies have characterized age-associated changes in lipids, it still remains incompletely understood how the lipidome changes across tissues and between sexes during aging. Here, we performed targeted lipidomics across 10 organs collected from male and female mice at five ages spanning adolescence to old age. We analyzed 775 lipids across multiple lipid classes and found that aging affects the lipidome in an organ-specific manner. The thymus and quadriceps muscle had the most age-associated lipid changes, whereas lipid levels in organs such as the kidney and lung remained more stable. In quadriceps muscle, aging was associated with a decrease in specific phosphatidylcholine and phosphatidylethanolamine lipids, particularly those containing adrenic acid. We also identified sex-dependent differences in lipid composition, with the spleen showing differences throughout life. Spleens from female mice had lower levels of lysophosphatidylcholine and lysophosphatidylethanolamine compared to males. Together, these data provide a comprehensive atlas of age- and sex-associated lipid changes across mouse organs and complement existing metabolic and transcriptomic resources to support studies of mouse aging.
    DOI:  https://doi.org/10.64898/2026.02.02.703178
  17. Transl Psychiatry. 2026 Feb 08.
      Lipids are highly abundant in the brain and play key roles in membrane regulation, neurotransmission, neurogenesis, and inflammation. The same processes are involved in neuromodulation mechanisms. While neuromodulation therapies have shown promising outcomes for treatment-resistant psychiatric disorders, the factors determining individual variability in treatment response remain poorly understood. Furthermore, the potential impact of neurometabolic factors in predicting response has been largely overlooked. This narrative review aims to evaluate the role of lipids in psychiatric neuromodulation. Particularly glycerophospholipids, sphingolipids and polyunsaturated fatty acids (PUFAs) have been described as important mediators. Current evidence suggests a bidirectional relationship between lipids and neuromodulation therapies such as electroconvulsive therapy (ECT), and repetitive transcranial magnetic stimulation (rTMS). Neuromodulation effects are associated with lipid metabolism changes, including phospholipids, sphingolipids, and fatty acids. ECT is associated with an increase in lipid peroxidation and alterations of cholesterol and fatty acid levels, while rTMS is associated with normalization of sphingolipids and phospholipids levels. Solely one study investigated the relation between deep brain stimulation and lipids, showing an association with sphingolipid metabolism. To our knowledge, this is the first comprehensive review to consolidate findings on the relationship between lipids and neuromodulation. By mapping this emerging field, these findings might be a first step towards investigating whether lipids could be a potential biomarker for response prediction in the future. As most findings are preliminary, with variability across studies, further investigation is warranted and current findings should be interpreted in the context of their limitations.
    DOI:  https://doi.org/10.1038/s41398-026-03873-2
  18. Cells. 2026 Jan 28. pii: 254. [Epub ahead of print]15(3):
      Peroxisomes are multifunctional organelles that play essential roles in lipid metabolism, redox regulation, and cellular signaling. An expanding body of evidence implicates peroxisomal dysfunction as a key contributor to aging and age-related diseases. Aging is accompanied by progressive declines in key peroxisomal functions, including catalase activity, fatty acid β-oxidation, plasmalogen biosynthesis, and the metabolism of bile acids and docosahexaenoic acid, resulting in increased oxidative stress, lipid dysregulation, and alterations in membrane composition. Impaired pexophagy further exacerbates these defects by allowing the accumulation of damaged peroxisomes and compromising cellular homeostasis. Through extensive metabolic and signaling crosstalk with mitochondria, the endoplasmic reticulum, and lysosomes, peroxisomal dysfunction can propagate oxidative and metabolic disturbances throughout the cell. In addition, peroxisome-derived signaling molecules, such as hydrogen peroxide and bioactive lipids, link peroxisomal activity to cellular stress responses and organismal metabolic homeostasis. We propose that aging-associated impairments in peroxisomal protein import, redox regulation, and selective turnover progressively shift peroxisomes from adaptive metabolic signaling hubs toward sources of chronic oxidative and lipid stress. In this context, current studies highlight peroxisomal homeostasis as a potential determinant of healthy aging and point to peroxisomal pathways as emerging targets for intervention in age-related disease.
    Keywords:  aging; catalase; interorganelle crosstalk; lipid metabolism; metabolic disorders; neurodegeneration; peroxisomes; pexophagy; reactive oxygen species; therapeutic interventions
    DOI:  https://doi.org/10.3390/cells15030254
  19. Alzheimers Dement. 2026 Feb;22(2): e70998
       INTRODUCTION: Epigenomic dysregulation contributes to Alzheimer's disease (AD) and related tauopathies. Acetyl-CoA synthetase 2 (ACSS2), a nuclear-localized metabolic enzyme in neurons, supports histone acetylation and learning-related gene expression. We examined how ACSS2 loss affects molecular and behavioral phenotypes in a mouse model of tauopathy.
    METHODS: We induced tauopathy in ACSS2 knockout and control mice via injection of pathological human tau. We assessed transcriptomic, epigenomic, and behavioral changes, and tested long-term acetate supplementation as a rescue strategy.
    RESULTS: ACSS2 loss worsened tau-seeding-related phenotypes, particularly in hippocampal pyramidal neurons and Cajal-Retzius cells. Acetate supplementation rescued learning in an ACSS2-dependent manner and restored gene expression linked to cognition.
    DISCUSSION: ACSS2 acts as a neuroprotective metabolic enzyme in vulnerable hippocampal neurons, and targeting this pathway through dietary supplementation may offer therapeutic potential for AD and related tauopathies.
    HIGHLIGHTS: We combine tau seeding with deletion of acetyl-CoA synthetase 2 (ACSS2) to test this enzyme in an Alzheimer's disease model. Loss of ACSS2 exacerbates transcriptional and behavioral responses to tau injection. We observe robust transcriptional dysregulation in pyramidal neurons in the hippocampus. We observe reduced numbers of reelin-producing Cajal-Retzius cells in the hippocampus. Acetate supplementation rescues transcriptional and behavioral responses to tau.
    Keywords:  Alzheimer's disease; RNAseq; acetyl‐CoA synthetase 2; assay for transposase‐accessible chromatin using sequencing; epigenetics; histone acetylation; learning and memory; single‐nucleus sequencing; tauopathy
    DOI:  https://doi.org/10.1002/alz.70998
  20. Amyotroph Lateral Scler Frontotemporal Degener. 2026 Feb 12. 1-27
      Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by progressive motor neuron loss and profound systemic metabolic dysfunction, including hypermetabolism, weight loss, insulin resistance, and altered glucose and lipid homeostasis. Increasing recognition of these metabolic abnormalities has driven interest in repurposing antidiabetic therapies, particularly glucagon-like peptide-1 (GLP-1) and GLP-1 receptor agonists (GLP-1RAs), for ALS. Beyond their established metabolic actions, GLP-1RAs exert pleiotropic effects relevant to neurodegeneration, including modulation of neuroinflammation, mitochondrial function, oxidative stress, excitotoxicity, and cell-survival signaling, with selected agents demonstrating central nervous system penetration. This narrative review summarizes current knowledge on metabolic impairment in ALS and critically evaluates the mechanistic rationale, preclinical evidence, and emerging clinical data supporting or opposing the use of GLP-1-based therapies in this disease. Preclinical studies suggest that GLP-1 signaling can provide neuroprotective and neurotrophic effects in ALS models, although findings are heterogeneous and highly dependent on compound selection, delivery strategy, and experimental design. In contrast, available clinical evidence is limited and does not demonstrate therapeutic benefit in ALS, while raising important safety concerns, particularly related to weight loss, lean mass reduction, and altered glucose regulation, factors associated with a worse prognosis in ALS. Collectively, current data indicate that although GLP-1-based therapies may have compelling biological plausibility and beneficial effects in other neurodegenerative disorders (NDGs), their role in ALS remains uncertain and potentially harmful. Well-designed, ALS-specific clinical studies are required to clarify safety, efficacy, and patient selection before GLP-1RAs can be considered for therapeutic use in this vulnerable population.
    Keywords:  Amyotrophic lateral sclerosis (ALS); Exendin-4 (Ex-4); Glucagon-Like Peptide-1 (GLP-1); Glucagon-Like Peptide-1 Receptor Agonists (GLP-1RAs); Hypermetabolism; Metabolic dysfunction; Motor Neurone Disease (MND); Neurodegenerative diseases (NDGs); Neuroprotection; Weight loss
    DOI:  https://doi.org/10.1080/21678421.2026.2627901
  21. J Biol Chem. 2026 Feb 05. pii: S0021-9258(26)00114-6. [Epub ahead of print] 111244
      Lipoxygenases (ALOX) are non-heme iron containing dioxygenases that catalyze the oxygenation of polyenoic fatty acid containing lipids to their corresponding hydroperoxy derivatives. These enzymes are widely distributed in highly developed plants and animals. In bacteria they rarely occur but they have not been detected in archaea and viruses. The human genome involves six functional ALOX genes (ALOX15, ALOX15B, ALOX12, ALOX12B, ALOXE3, ALOX5) encoding for six different isoenzymes. The mouse genome carries an orthologous gene for each human ALOX gene but in addition an Aloxe12 gene has been identified in this species. The application of isoenzyme-specific loss-of-function strategies suggested that the coding multiplicity may not be interpreted as sign of functional redundancy. In fact, the different isoenzymes apparently fulfill different biological functions. Mammalian ALOX15 orthologs are allosteric enzymes but the molecular basis for their allosteric properties remains controversial. In fact, two alternative hypotheses (presence of allosteric binding sites at enzyme monomers vs. ALOX15 dimers consist of an allosteric and a catalytic monomer) have been introduced and this review is aimed at critically evaluating the pros and conts of these two mechanistic scenarios.
    Keywords:  atherosclerosis; cancer; eicosanoids; inflammation; lipid peroxidation; lipoxygenase; neurodegeneration
    DOI:  https://doi.org/10.1016/j.jbc.2026.111244