bims-micpro 2022-01-09 papers

STAR Protoc. 2022 Mar 18. 3(1): 101037

Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines.

Cai Han, Linyu Sun, Qi Pan, Yumeng Sun, Wentao Wang, Yueqin Chen.

Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).

Keywords: Cell separation/fractionation; Molecular Biology

DOI: https://doi.org/10.1016/j.xpro.2021.101037