bims-mignad Biomed News
on Mitochondria galactose NAD
Issue of 2024‒11‒10
two papers selected by
Melisa Emel Ermert, Amsterdam UMC



  1. Ageing Res Rev. 2023 Oct 28. pii: S1568-1637(23)00265-9. [Epub ahead of print] 102106
      More than a century after the discovery of nicotinamide adenine dinucleotide (NAD+), our understanding of the molecule's role in the biology of ageing continues to evolve. As a coenzyme or substrate for many enzymes, NAD+ governs a wide range of biological processes, including energy metabolism, genomic stability, signal transduction, and cell fate. NAD+ deficiency has been recognised as a bona fide hallmark of tissue degeneration, and restoring NAD+ homeostasis helps to rejuvenate multiple mechanisms associated with tissue ageing. The progressive loss of skeletal muscle homeostasis with age is directly associated with high morbidity, disability and mortality. The aetiology of skeletal muscle ageing is complex, involving mitochondrial dysfunction, senescence and stem cell depletion, autophagy defects, chronic cellular stress, intracellular ion overload, immune cell dysfunction, circadian clock disruption, microcirculation disorders, persistent denervation, and gut microbiota dysbiosis. This review focuses on the therapeutic potential of NAD+ restoration to alleviate the above pathological factors and discusses the effects of in vivo administration of different NAD+ boosting strategies on skeletal muscle homeostasis, aiming to provide a reference for combating skeletal muscle ageing.
    Keywords:  NAD(+) boosting; NAD(+) metabolism; Skeletal muscle ageing; Therapies
    DOI:  https://doi.org/10.1016/j.arr.2023.102106
  2. STAR Protoc. 2024 Nov 01. pii: S2666-1667(24)00593-8. [Epub ahead of print]5(4): 103428
      Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD+ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD+ and NADH levels across different biological models.
    Keywords:  Cell Biology; Metabolism; Molecular Biology; Neuroscience
    DOI:  https://doi.org/10.1016/j.xpro.2024.103428