bims-migras 2025-06-22 papers

Issue of 2025–06–22
five papers selected by
Cliff Dominy

Angiogenesis. 2025 Jun 16. 28(3): 34

Diversity of extracellular vesicle sources in atherosclerosis: role and therapeutic application.

Yuan Zhang, Wendiao Zhang, Zhiwen Wu, Yong Chen.

Extracellular vesicles (EVs) are phospholipid bilayer membrane structures secreted by cells and widely present in blood or body fluids, playing critical roles in cell communication and homeostasis. Increasing evidence has implicated EVs dysfunction in the pathogenesis of various cardiovascular diseases (CVD), including atherosclerosis (AS), ischemic heart disease, heart failure, aortic lesions, and valvular lesions. Using EVs derived from diseases or multiple tissue types to illuminate the functional mechanisms of EVs will promote pathological studies and drug development. EVs including exosomes, microvesicles, and apoptotic bodies, play key roles in the cellular physiological processes linked to AS, notably the recently developed engineering strategies applied to EVs have provided a new avenue for elucidating the mechanisms underlying the development and pathology of AS. To help researchers develop robust and reproducible methods that recapitulate in-vivo signatures of EVs to study AS development and pathology, this review summarized the current methods used to isolate or generate EVs and provided opinions on the use of EVs for disease and functional studies through collecting EVs derived from different kinds of cells or diseases in AS, which are the aspects that have not been generalized in previous reviews. In essence, EVs and their derivatives offer a novel approach to understanding the complex etiology of AS, and serve as a substantial basis for the discovery of potential diagnostic biomarkers and therapeutic targets.

Keywords: Atherosclerosis; Cardiovascular diseases; EV cargoes; Extracellular vesicles

DOI: https://doi.org/10.1007/s10456-025-09983-7

J Cardiovasc Transl Res. 2025 Jun 19.

Exosomal miRNAs: A New Frontier in Cardiovascular Disease Diagnosis and Treatment.

Sun Qingpiao, Zhang Yi.

Exosomes are small vesicles secreted by a variety of cells surrounded by a lipid bilayer membrane and containing a variety of biomolecules, of which microRNAs (miRNAs) are the most abundant. Exosomal miRNAs are involved in the development of cardiovascular diseases(CVD), and their unique biological properties make them expected to be effective targets for the treatment of CVD. This article reviews the biological characteristics, expression, and mechanism of action of exosomes and exosomal miRNAs in CVD, as well as their potential as biomarkers in the diagnosis, treatment, and prognostic assessment of CVD, to provide new ideas in the field of CVD medicine.

Keywords: Biomarker; Cardiovascular disease; Drug carrier; Exosomal miRNAs

DOI: https://doi.org/10.1007/s12265-025-10617-y

J Extracell Vesicles. 2025 Jun;14(6): e70112

Single-Cell Analysis Reveals Fibroblast-Derived Migrasomes as CXCL12 Carriers Promoting Skin Wound Repair.

Haoyu Zhou, Zhen Zhang, Zhan Liu, Guodong Sa, Mingjing Jiang, Zhongyang Zou, Yingliang Shi, Liwu Zheng, Xuewen Yang, Guoliang Sa.

Migrasomes are newly discovered organelles with demonstrated functions in organ morphogenesis and angiogenesis. However, the effect of migrasomes in tissue repair remains unreported. Our super-resolution confocal microscopy and focused ion beam scanning electron microscopy results confirmed that migrasomes were directly connected with retraction fibres and could release their contents into the surroundings in human and rat skins and oral mucosae. Multiplex immunofluorescence staining results revealed that these retraction fibres and migrasomes originated from fibroblasts. Live-cell imaging demonstrated that human oral mucosal fibroblast-derived migrasomes could be taken up by both fibroblasts and HaCaT cells. In addition, the injection of purified fibroblast-derived migrasomes into the edges of rat skin wounds significantly accelerated wound healing. Single-cell sequencing results suggested that the clusters of keratinocytes, fibroblasts, and endothelial cells play key roles in the wound-healing process. Moreover, the expression of Vegfa, Il-6, and Col1a1 in the fibroblast subcluster was significantly upregulated. Furthermore, these purified migrasomes increased the protein levels of VEGFA, IL-6, and COL1A1 in cultured fibroblasts in vitro. Mechanistically, migrasomes may facilitate wound healing by delivering CXCL12. Thus, our research revealed that fibroblast-derived migrasomes are potential therapeutic vesicles for skin wound-healing repair.

Keywords: extracellular vesicles; migrasome; single‐cell RNA sequence; skin wound healing

DOI: https://doi.org/10.1002/jev2.70112

Clin Chim Acta. 2025 Jun 12. pii: S0009-8981(25)00303-1. [Epub ahead of print] 120424

Circular RNA biomarkers in cardiovascular disease.

Tursunova Nozima, Nassyrova Nargiza Batyrkhankyzy, Mustafa Jawad Kadham, Khamidova Aziza Abdufattoevna, Alijon Khatamov, Pulatova Shaxnoza Khaydarova, Ibragimkhodjaev Bakhodir, Rasulova Nodira Alisherovna, Kholmurodova Dilafruz Kuvatovna, Khidoyatova Mukhlisa, Kudeshova Gulchekhra, Bobojonov Otabek, Matkarimov Inomjon.

Despite medical advancements, heart disease continues to be a primary cause of death globally. While early detection and continuous monitoring are essential for better patient outcomes, current biological markers, such as cardiac troponins and BNP, have shortcomings, including their temporary nature and susceptibility to various factors, such as patient age and other medical conditions. This limitation has motivated scientists to identify new biological indicators and treatment targets that better represent the underlying heart disease mechanisms. Circular RNAs (circRNAs) have recently been identified as key regulatory molecules in various illnesses, including cardiac conditions. These unique noncoding RNA molecules feature a closed circular structure that makes them exceptionally stable and resistant to breakdown. Their durability, specific expression patterns in different tissues, and preservation across species make them promising candidates both as disease markers and potential therapeutic tools for heart conditions. The scientific interest in the role of circRNAs in cardiovascular disease has increased significantly, with studies revealing their involvement in controlling genes and disease development. This comprehensive review examines circRNAs in heart disease, covering their formation, functional mechanisms, and potential clinical applications as disease markers. This paper discusses recent scientific discoveries highlighting their value in diagnosis and prognosis, addresses the difficulties in moving these findings into medical practice and explores future possibilities for the use of circRNAs in heart disease diagnosis and treatment.

Keywords: Biomarkers; Circular RNAs; Clinical Applications; Heart Disease

DOI: https://doi.org/10.1016/j.cca.2025.120424

Transl Androl Urol. 2025 May 30. 14(5): 1214-1229

Rong Chen, Lei Dong, Tianci Wei, Qiang Wang.

Background: Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive renal malignancy. Migrasomes, newly discovered organelles involved in intercellular communication, and long non-coding RNAs (lncRNAs) are emerging regulators of cancer progression. However, the role of migrasome-associated lncRNAs in ccRCC prognosis and immune response remains unclear. This study aimed to investigate the value of migrasome-related lncRNAs and develop a risk model for ccRCC.
Methods: By employing data from The Cancer Genome Atlas, we were able to identify prognostically significant migrasome-related lncRNAs through co-expression analysis, Cox regression, and least absolute shrinkage and selection operator (LASSO) regression. Prognostic models were developed and validated using these lncRNAs, and a nomogram combining the risk score with clinical features was constructed. Furthermore, our analyses encompassed gene set enrichment, immune infiltration, mutational burden, and drug sensitivity.
Results: A prognostic model incorporating 13 lncRNAs effectively stratified patients into distinct risk categories, with the high-risk cohort demonstrating markedly inferior survival rates. The prognostic accuracy was validated through multiple analyses. Gene enrichment analysis revealed a correlation between these lncRNAs and tumor development and immune pathways. High-risk patients exhibited increased immunosuppressive cell infiltration, oncogenic mutations, and potential for immune escape. Furthermore, they demonstrated a lack of response to immunotherapy and exhibited differential responses to antineoplastic agents when compared to low-risk patients. We propose a prognostic model for ccRCC based on migrasome-related lncRNAs, providing new insights into disease progression and potential individualized treatment strategies.
Conclusions: Our study proposes a prognostic model for ccRCC based on migrasome-related lncRNAs, providing new insights into disease progression and potential individualized treatment strategies.

Keywords: Migrasome; clear cell renal cell carcinoma (ccRCC); drug sensitivity; immune; prognosis

DOI: https://doi.org/10.21037/tau-2024-728