bims-plasge 2025-07-13 papers

Issue of 2025–07–13
two papers selected by
Vera S. Bogdanova, ИЦиГ СО РАН

Theor Appl Genet. 2025 Jul 08. 138(8): 179

QTL combinations associated with field partial resistance to Aphanomyces root rot in pea near-isogenic lines.

C Lavaud, J-P Riviere, P Vetel, G Aubert, P Marget, G Roullet, J F Herbommez, P Declerck, M-L Pilet-Nayel.

Aphanomyces root rot, caused by Aphanomyces euteiches, is one of the most-important diseases of pea (Pisum sativum L.) worldwide. The development of resistant varieties is a major objective to manage the disease. Consistent quantitative trait loci (QTL) controlling partial resistance were discovered from linkage mapping and genome-wide association studies. This study aimed to validate the resistance QTL effects and identify effective QTL combinations under contaminated field conditions, by exploiting near-isogenic lines (NILs) carrying resistance alleles at individual or combined consistent QTL in different genetic backgrounds. A total of 157 NILs previously created were fingerprinted using 10,494 SNP markers from the GenoPea Infinium® BeadChip, which made it possible to confirm the QTL introgression sizes in the NILs. All NILs were phenotyped for resistance in field-contaminated nurseries over eight environments in 2 years at six locations in France. NILs carrying resistance alleles from PI180693 or 90-2131 at the major-effect QTL Ae-Ps7.6, individually or in combination with minor-effect QTL (Ae-Ps4.1 or Ae-Ps5.1), showed increased levels of partial resistance in at least three environments and three genetic backgrounds. At other QTL combinations (Ae-Ps1.2 or Ae-Ps7.6 + Ae-Ps2.2 ± Ae-Ps3.1), alleles from PI180693 or 552 also showed consistent and novel effects on partial resistance in some NIL genetic backgrounds. At these QTL combinations, the PI180693 resistance alleles also contributed to late flowering. This study provides tools and information for the choice of resistance QTL to combine in breeding, to increase partial resistance to A. euteiches in pea varieties.

DOI: https://doi.org/10.1007/s00122-025-04961-5

J Exp Bot. 2025 Jul 08. pii: eraf309. [Epub ahead of print]

Multiple fertility restorer loci for cytoplasmic male sterility caused by orf137 in tomato.

Yurie Iki, Issei Harada, Kentaro Ezura, Seira Mashita, Kosuke Kuwabara, Hitomi Takei, Atsushi Toyoda, Kenta Shirasawa, Tohru Ariizumi.

Cytoplasmic male sterility (CMS) in plants is caused by incompatibility between nuclear and cytoplasmic genetic information. Fertility can be restored through the action of fertility restoration (RF) genes, which are usually present in the nucleus. CMS lines of tomato (Solanum lycopersicum) have been developed from asymmetric cell fusions, in these lines, cultivated tomato served as a nuclear donor and its wild relative, S. acaule, as a cytoplasm donor. Although RF genes are present in wild relatives of tomato, no genetic or genomic information on the RF genes is yet available. This study reports an RF genetic locus, RF1, on chromosome 1 of S. pimpinellifolium LA1670 and S. lycopersicum var. cerasiforme LA1673 that was revealed by bulked segregant analysis and sequencing. An additional RF locus, RF2, was identified on chromosome 2 of LA1670. The genomic sequence of S. cheesmaniae LA0166 was assembled using high-fidelity, long-read sequencing technology. Sequence comparisons identified further candidate RF genes on chromosome 1 of S. cheesmaniae LA0166. These results suggested that multiple gene loci control the fertility restoration trait in wild relatives of tomato.

Keywords: Cytoplasmic male sterility; F1 seed production; Fertility restoration; Genome; Tomato; Transformation

DOI: https://doi.org/10.1093/jxb/eraf309