bims-traimu Biomed News
on Trained immunity
Issue of 2025–12–28
nine papers selected by
Yantong Wan, Southern Medical University



  1. J Clin Immunol. 2025 Dec 24.
      Trained immunity, a de-facto innate immune memory, has been extensively studied in response to live-attenuated vaccines, but its presence following the new COVID-19 vaccines has not yet been fully elucidated. In this study, we investigate markers of trained immunity in individuals vaccinated with mRNA-1273 or BNT162b2. As part of the vaccine roll-out in Denmark and recruited for a comparative study. Our primary objective was to determine whether these vaccines elicit lasting changes in innate immune responses, particularly in monocyte populations and cytokine production following stimulation with a panel of agonists. The study was conducted at four time points: Day-0 (pre-vaccination), Day-28, Day-90, and Day-180 post-vaccination. We observed no significant differences in monocyte subpopulations between vaccine groups; however, cytokine and chemokine analysis revealed distinct immune signatures. While IL-6 and TNFα production remained unchanged after ex-vivo restimulation in the BNT162b2 group, individuals vaccinated with mRNA-1273 exhibited a sustained increase in the production of these cytokines, persisting for up to 180 days post-vaccination. Additionally, CCL2, a key chemokine involved in monocyte recruitment, was upregulated following mRNA-1273 vaccination but decreased in the BNT162b2 group, further supporting the finding of differential innate immune responses between the two vaccines. In conclusion, our study provides evidence that mRNA-1273, but not BNT162b2, induces immune responses consistent with the concept of trained immunity. These results highlight the potential for mRNA vaccine platforms to shape innate immunity, with implications for future vaccine design aimed at enhancing non-specific and specific protection against infectious diseases.
    Keywords:  BNT162b2; Cytokine response; Monocytes; Trained immunity; mRNA vaccines; mRNA-1273
    DOI:  https://doi.org/10.1007/s10875-025-01977-w
  2. J Transl Med. 2025 Dec 23. 23(1): 1412
       BACKGROUND: Acute lung injury (ALI) is a life-threatening clinical syndrome typically triggered by sepsis or severe trauma lacking effective treatment options. Alveolar macrophages (AMs), representing the most abundant immune cell population in pulmonary tissue, exhibited functional abnormalities that were closely associated with ALI pathogenesis. Notably, elevated pulmonary lactate secretion served not only as a characteristic pathological feature of ALI but also participated in disease progression through modulation of AMs activity. Trained immunity was found to activate innate immune cells including macrophages, regulating metabolic adaptations that alleviated ALI, though the precise mechanisms remained unclear.
    METHODS: We used β-glucan and LPS to establish both in vivo and in vitro models of trained immunity and ALI, enabling investigation of trained immunity effects on AMs immunoregulatory functions.
    RESULTS: The results demonstrated that trained immunity effectively attenuated ALI severity by up-regulating glycolytic activity in AMs, thereby potentiating their immune responsiveness, and primarily enabled alveolar macrophages to sustain immune responses in high-lactate environments through the AKT2-PDK1 axis, an effect that was abolished by relevant inhibitors.
    CONCLUSIONS: We concluded that β-glucan induced trained immunity could enhance alveolar macrophage immune activity and improve lactate metabolic tolerance, offering a novel therapeutic approach for acute lung injury (ALI).
    Keywords:  AKT2; Acute lung injury; Alveolar macrophages; Lactate; Pyruvate dehydrogenase kinase 1; Trained immunity
    DOI:  https://doi.org/10.1186/s12967-025-06879-4
  3. Elife. 2025 Dec 23. pii: e108465. [Epub ahead of print]14
      Trained Immunity is the nonspecific (pathogen agnostic) memory of innate immune cells, characterized by altered responses upon secondary stimulation. This review provides a RoadMap for the discovery and development of therapeutics targeting Trained Immunity, aimed at researchers with strong scientific backgrounds but limited drug development experience. The article outlines five drug development domains - epigenetic, metabolic, differentiation, inflammatory, and memory changes - that guide the identification of molecular targets, model selection, and biomarker development for the discovery and development of Trained Immunity-based therapeutics. It emphasizes the application of preclinical models and artificial intelligence in target discovery and compound screening. Additionally, the review addresses challenges in translating preclinical Trained Immunity findings to clinical trials and highlights relevant disease indications and ongoing clinical trials. This review integrates scientific findings with development strategy and thereby aims to bridge the gap between discovery and clinical application, advancing the field of Trained Immunity-based therapeutics.
    Keywords:  Trained Immunity; drug development; drug discovery; immunology; inflammation
    DOI:  https://doi.org/10.7554/eLife.108465
  4. J Periodontol. 2025 Dec 26.
      Historically, immunological memory was considered an exclusive feature of adaptive immunity. However, innate immune cells have recently been shown to record and maintain epigenetically imprinted memory of earlier infectious or inflammatory challenges. In the bone marrow, hematopoietic stem and progenitor cells (HSPCs) can also build inflammatory memory, which leads to trained myelopoiesis, that is, the production of high numbers of myeloid cells with increased inflammatory responsiveness to future challenges. Another condition affecting HSPCs and causing overproduction of leukocytes with increased inflammatory responsiveness is clonal hematopoiesis of indeterminate potential (CHIP). Occurring at high prevalence in the elderly, CHIP is driven predominantly by somatic mutations in genes encoding epigenetic modifiers, hence altering the epigenetic landscape of hematopoietic progenitors and their mature progeny. Although trained myelopoiesis and CHIP may have beneficial effects, they can also act in a maladaptive context and aggravate inflammation in periodontal disease and systemic conditions, thereby forming a common mechanistic basis for inflammatory comorbidities. This review discusses recent experimental and clinical evidence on the mechanisms and implications of maladaptive hematopoiesis-due to trained myelopoiesis or CHIP--in periodontitis and associated inflammatory comorbidities. PLAIN LANGUAGE SUMMARY: Traditionally, immune memory-the ability to "remember" past infections-was thought to be limited to the adaptive immune system. But recent discoveries show that even the more ancient part of the immune system, the innate immune system, can also develop a form of memory. This memory is stored through changes in how genes are regulated, and it begins in the bone marrow. There, blood-forming stem cells can be "trained" by previous infection or inflammation to produce more immune cells that respond more aggressively to future threats, a process known as trained myelopoiesis. An analogous process happens in a condition called clonal hematopoiesis of indeterminate potential (CHIP), which is common in older adults. In CHIP, mutations in certain genes cause the bone marrow to overproduce immune cells that are overly reactive. While trained myelopoiesis and CHIP may exert protective effects, they can also backfire. Both trained myelopoiesis and CHIP have been linked to increased inflammation in gum disease and associated systemic conditions. This review explores how bone marrow-driven changes in the production and activity of immune cells may contribute to a shared underlying cause of multiple inflammatory disorders and why understanding these processes could open new doors for treatment and prevention.
    Keywords:  clonal hematopoiesis; comorbidities; inflammation; periodontitis; trained immunity
    DOI:  https://doi.org/10.1002/jper.70040
  5. NPJ Vaccines. 2025 Dec 26.
      SARS-CoV-2 immunity and innate immune training may influence influenza vaccine immunogenicity. We investigated this in India. Adult volunteers with hybrid SARS-CoV-2 immunity were administered FluarixTM Tetra (GlaxoSmithKlein) 2022/2023 NH Vaccine in 2022. Significant induction of hemagglutinin inhibition-specific antibodies and polyfunctional central memory CD4+ T-cells (TCM) were observed 1-week post-vaccination with variable induction of CD8+T-cell and innate effectors. Vaccination also expanded Flu-specific regulatory T-cells (Treg), which negatively correlated with CD4 responses, highlighting vaccine immunogenicity may be subject to Treg dampening. FluarixTM did not boost SARS-CoV-2 immunity. However, SARS-CoV-2-specific T-cell responses correlated positively with vaccine-induced T-cell responses. We evaluated trained immunity post-COVID-19 as a potential regulatory mechanism linking SARS-CoV-2 and heterologous vaccine immunogenicity. We observed, elevated frequencies of basal bacterial Lipopolysaccharide (LPS)-induced IL-6+IL1β+HLA-DR+CD14+CD16- frequencies post-COVID-19 correlated positively with vaccine-induced Fluarix-specific CD4 T-cell frequencies. Our study highlights a potential positive role for COVID-19-driven immune imprinting on heterologous vaccine immunogenicity in a post-COVID-19 era.
    DOI:  https://doi.org/10.1038/s41541-025-01331-6
  6. Front Immunol. 2025 ;16 1707719
      Macrophages are key innate immune cells responsible for initiating and coordinating immune responses. A major determinant of macrophage function is their tissue-context-dependent polarization state, regulated by the cytokines present in the tissue microenvironment. Yet, the capability of characterizing macrophage polarization states in clinical studies remains limited. Here, we used a defined set of cytokines to polarize human PBMC-derived macrophages and determine their transcriptional signatures and stimulus responsiveness. The resultant atlas of transcriptional signatures for human macrophage polarization states was applied to a dataset of intestinal biopsies from Crohn's disease patients and healthy controls. Our analysis identified a dominant population of IFNγ-polarized macrophages in areas of active Crohn's intestinal inflammation and a loss of wound healing IL-4-, IL-10- and IL-13-polarized macrophages. This study demonstrates that in vitro datasets of macrophages in defined conditions can be leveraged to interpret the functionality of cells transcriptional profiled in clinical studies.
    Keywords:  Crohn’s disease; inflammatory bowel disease; macrophage polarization; macrophages; mucosal immunology; scRNAseq
    DOI:  https://doi.org/10.3389/fimmu.2025.1707719
  7. Sci Rep. 2025 Dec 24. 15(1): 44501
      Severe influenza is frequently associated with bacterial or viral co-infections. Although immune-mediated mechanisms have been extensively investigated, the cell-intrinsic responses of respiratory epithelial cells prior to immune engagement remain poorly characterized. To model co-infection in vitro, we pre-activated B16 melanoma cells using the TLR9 agonist CpG ODN, followed by infection with influenza A virus (IAV) strain FM1. Key molecular events, including STING expression and activation, were assessed by Western blotting and RT-qPCR. The in vivo relevance was assessed in a mouse model of intranasal CpG ODN pre-treatment and IAV challenge. RNA-seq was performed as a supplementary approach to explore possible cell death pathways. Pre-activation significantly enhanced IAV-induced cell death within a restricted vulnerability window (≤ 24 h), independent of viral replication or TLR9 signaling. This was associated with inflammatory priming and initial STING activation. However, IAV infection subsequently suppressed STING phosphorylation and elevated inflammatory cell death. Mice pre-treated with CpG ODN exhibited increased mortality and aggravated lung injury following IAV challenge. RNA-seq analysis suggested the potential involvement of pyroptosis. These findings demonstrate that cellular pre-stimulation exacerbates influenza severity by promoting inflammatory cell death, a process mechanistically linked to the subsequent suppression of STING activation. This study reveals a novel pathogenic cell-intrinsic mechanism in co-infection pathogenesis.
    Keywords:  Cell death; Infection; Influenza virus; Pre-activation; STING
    DOI:  https://doi.org/10.1038/s41598-025-28063-w
  8. Nat Commun. 2025 Dec 23.
      Necrosome assembly is essential for necroptosis, a process implicated in neurodegeneration, ischemic injury, and inflammatory diseases. Yet the spatiotemporal rules governing this assembly remain elusive. Leveraging quantitative STORM and mathematical modeling, we define an approximately 3:1 ratio of RIP3 to RIP1 in necrosomes as the optimal stoichiometry for necroptosis, enabling signal amplification and a threshold response. Surprisingly, excessive RIP3 oligomerization attenuates signaling, acting as an intrinsic size control mechanism. RIP3 assembly is dynamically regulated: it is constrained by stimulation and RIP1, promoted by RIP3 itself, and unexpectedly limited by downstream MLKL. A complementary balance between necrosome quantity and RIP3 assembly degree ensures efficient MLKL phosphorylation. In contrast, Caspase-8 assembly is limited by c-FLIP and recruited linearly by RIP1, while its distinct behavior from RIP3 underlies the biphasic necroptotic response to RIP1. These findings uncover the flexible, multi-strategic nature of signalosomes and offer valuable insights for therapeutic and synthetic biology.
    DOI:  https://doi.org/10.1038/s41467-025-67098-5
  9. Research (Wash D C). 2025 ;8 1011
      Sepsis is a life-threatening syndrome caused by dysregulated host response that culminates in organ dysfunction. Recognizing that many prior reviews treated neutrophils as largely homogeneous or discussed their functions in isolation, we present a framework centered on emergency granulopoiesis that unifies groundbreaking single-cell multi-omics discoveries, bone marrow niche remodeling, and bedside biomarkers such as the immature-to-total neutrophil ratio and sepsis endotypes to explain the shift from defense to dysfunction. As a hallmark adaptation, emergency granulopoiesis rapidly replenishes neutrophils but paradoxically yields immature neutrophils with impaired chemotaxis, excessive neutrophil extracellular trap formation (NETosis)/reactive oxygen species, and immunosuppressive activity that aggravate tissue damage, organ failure, and late immunoparalysis. We synthesize subset-level diversity and map this diversity to transcriptional shifts coupled with epigenetic and metabolic reprogramming as well as stromal inflammation. Clinically, elevated immature-to-total neutrophil ratios and these immature subsets align with disease severity and mortality, supporting biomarker-guided stratification. Stage-specific therapeutic strategies are evaluated along the continuum of emergency granulopoiesis, calibrating NETosis, and modulating metabolic reprogramming and epigenetics to restore neutrophil homeostasis. Furthermore, we delineate testable priorities for future research such as standardizing immature neutrophil phenotyping across cohorts and defining harmonized immature-to-total neutrophil ratio thresholds. By explicitly centering emergency granulopoiesis and integrating single-cell biology with bedside indicators, this review clarifies why a protective program becomes maladaptive in sepsis and outlines practical avenues for targeted interventions and trial design.
    DOI:  https://doi.org/10.34133/research.1011